Method for debonding sticky fertilized eggs of marine fishes
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A fertilized egg, seawater technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of low hatching rate, incompleteness, sticky eggs easy to agglomerate, etc.
Inactive Publication Date: 2020-11-13
ZHEJIANG MARICULTURE RES INST
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[0003] 1) Surface mucus makes sticky eggs translucent or opaque, and their embryonic development cannot be observed through a microscope. In scientific research work, the phylogeny of these fish cannot be studied, and the hatching time cannot be grasped in time in production, which in turn causes breeding. confusion in work schedules;
[0004] 2) Sticky eggs are easy to agglomerate during the hatching process, resulting in ...
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Embodiment 1
[0020] Taking the viscous fertilized eggs of redfin puffer as an example, they were debonded with tannic acid seawater solution.
[0021] Step 1: preparing a debonding solution; using seawater as a solvent and tannic acid as a solute, preparing a 0.05% tannic acid seawater solution;
[0022] Step 2: aerating and immersing; immersing the fertilized eggs in the tannic acid seawater solution, inflating the water at the same time, fully immersing for 30 seconds;
[0023] Step 3: cleaning and counting; take out the fertilized eggs after soaking, place them in clean seawater for cleaning, and obtain debonded fertilized eggs, then put the debonded fertilized eggs into a petri dish, add clean seawater , let stand for 10min, pour off the seawater, count, and calculate the peeling rate.
[0024] The debonded fertilized eggs were microscopically observed under a dissecting microscope, and then the debonded fertilized eggs were hatched: 200 fertilized eggs were placed in clean seawater i...
Embodiment 2
[0026] Taking the viscous fertilized eggs of puffer puffer as an example, they were debonded with tannic acid seawater solution.
[0027] The mass concentration of the tannic acid seawater solution used is 0.05%, and the time for aerated soaking is 20s. Other operations are the same as in Example 1.
Embodiment 3
[0029] Taking the viscous fertilized eggs of puffer puffer as an example, they were debonded with tannic acid seawater solution.
[0030] The mass concentration of the tannic acid seawater solution used is 0.05%, and the time for aerated soaking is 20s. Other operations are the same as in Example 1.
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Abstract
Aiming at the adverse effect of sticky fertilized eggs on artificial propagation and scientific research, the invention provides a method for debonding sticky fertilized eggs of marine fishes. According to the method, a tannic acid seawater solution with a specific concentration is used for debonding the sticky fertilized eggs, the method comprises the steps of debonding solution preparation, inflating embathe, washing, counting and the like, and finally, the debonded fertilized eggs are artificially hatched, so that the hatchability is remarkably improved. The method is good in debonding effect, the debonding of fertilized eggs is uniform and thorough, damage to the fertilized eggs is reduced to the maximum extent, the hatching rate of the sticky fertilized eggs of artificially cultured marine fishes is increased, and the technological method is simple and high in debonding speed.
Description
technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for fertilizing and hatching seawater fish that lay sticky eggs. Background technique [0002] Sticky eggs refer to fish eggs with mucus or sticky filaments on the surface of the egg membrane. It is mainly found in freshwater carps, migratory salmon and trout, and marine fishes such as pufferiformes. These eggs often adhere to aquatic plants, seaweeds or ocean reefs after laying. These sticky eggs are suitable for artificial breeding and Relevant studies have brought adverse effects, mainly as follows: [0003] 1) Surface mucus makes sticky eggs translucent or opaque, and their embryonic development cannot be observed through a microscope. In scientific research work, the phylogeny of these fish cannot be studied, and the hatching time cannot be grasped in time in production, which in turn causes breeding. confusion in work schedules; [0004] 2) Sticky eggs are e...
Claims
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