Antibacterial auxiliary material having bacterial conditioning characteristic as well as preparation method and application of antibacterial auxiliary material
A technology of auxiliary materials and bacteria, applied in the field of pharmacy, to achieve the effect of protecting toxic and side effects, improving antibacterial efficacy, and promoting phagocytosis
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Embodiment 1
[0032] 2-Chlorobenzotrichlorotoluene resin (0.4 mmol, 100-200 mesh, 1% DVB) was placed in a synthesis column and immersed in DCM until the resin was completely dissolved. Fmoc-L-lys-OH was introduced into the reactor, and 0.5 mL of HBTU was used as the condensation agent. After reacting for 2 h under nitrogen stirring, the resin was washed 3 times with DMF and DCM. Then, 15 ml of the deprotection solution was added to the obtained Fmoc-protected amino acid resin. Subsequently, the Fmoc-amino acid-OH was reacted with the resin by the condensing agent HBTU according to the amino acid sequence from the C-terminus to the N-terminus in the expected lipopolysaccharide-binding protein mimetic peptide WKVRKSFFKLQGK. After each condensation reaction, the resin was washed alternately with DMF and methanol. A portion of the resin was then removed and examined by the ninhydrin method. The condensation is complete when the resin develops a pale yellow color. The resulting polypeptide w...
Embodiment 2
[0035] 2-Chlorobenzotrichlorotoluene resin (0.4 mmol, 100-200 mesh, 1% DVB) was placed in a synthesis column and immersed in DCM until the resin was completely dissolved. Fmoc-amino acid(biotin)-OH was introduced into the reactor, and 0.5 mL of HBTU was used as a condensing agent. After reacting for 2 h under nitrogen stirring, the resin was washed 3 times with DMF and DCM. Then, 15 ml of the deprotection solution was added to the obtained Fmoc-protected amino acid resin. Subsequently, Fmoc-amino acid-OH was reacted with the resin by the condensing agent HBTU according to the amino acid sequence from the C-terminus to the N-terminus in the expected mimetic peptide FHRNHRSPVTLL. After each condensation reaction, the resin was washed alternately with DMF and methanol. A portion of the resin was then removed and examined by the ninhydrin method. The condensation is complete when the resin develops a pale yellow color. The resulting polypeptide was cleaved from the resin and p...
Embodiment 3
[0037] 2-Chlorobenzotrichlorotoluene resin (0.4 mmol, 100-200 mesh, 1% DVB) was placed in a synthesis column and immersed in DCM until the resin was completely dissolved. Fmoc-L-lys-OH was introduced into the reactor, and 0.5 mL of HBTU was used as the condensation agent. After reacting for 2 h under nitrogen stirring, the resin was washed 3 times with DMF and DCM. Then, 15 ml of the deprotection solution was added to the obtained Fmoc-protected amino acid resin. Subsequently, Fmoc-amino acid-OH was reacted with the resin by the condensing agent HBTU according to the amino acid sequence from the C-terminus to the N-terminus in the expected mimetic peptide FHRNHRSPVTLL. After each condensation reaction, the resin was washed alternately with DMF and methanol. A portion of the resin was then removed and examined by the ninhydrin method. The condensation is complete when the resin develops a pale yellow color. The resulting polypeptide was cleaved from the resin and purified b...
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