Porcine epithelial cell oxidative stress model, establishment method therefor and application of porcine epithelial cell oxidative stress model

An epithelial cell, oxidative stress technology, applied in artificial cell constructs, biochemical equipment and methods, epidermal cells/skin cells, etc., to reduce direct losses, maintain human health, and reduce indirect losses.

Active Publication Date: 2020-11-17
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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[0010] The purpose of the present invention is to provide a porcine epithelial cell oxidative stress model and its establishment method and application thereof, aiming to solve th

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  • Porcine epithelial cell oxidative stress model, establishment method therefor and application of porcine epithelial cell oxidative stress model
  • Porcine epithelial cell oxidative stress model, establishment method therefor and application of porcine epithelial cell oxidative stress model
  • Porcine epithelial cell oxidative stress model, establishment method therefor and application of porcine epithelial cell oxidative stress model

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Embodiment Construction

[0028] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] 1. Materials and methods

[0030] 1. Main materials

[0031]Porcine intestinal epithelial cells (IPEC-J2), 500ml double antibody complete medium: 450ml high glucose medium DMEM+50ml fetal bovine serum FBS+5ml double antibody (penicillin and streptomycin), basic DMEM, PBS pH7.2, HBSS, 0.25% Trypsin-EDTA, Beyotime Animal Genomic DNA Rapid Extraction Kit, Beyotime Reactive Oxygen Detection Kit, Beyotime EdU-488 Cell Proliferation Detection Kit, iTap Universal SYBR Green Supermix Quantitative Master Mix, Cell CountingKit- 8 Cell proliferation-toxicity detection kit, etc.

[0032] Among them, ...

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Abstract

The invention discloses a porcine epithelial cell oxidative stress model, an establishment method therefor and application of the porcine epithelial cell oxidative stress model. The establishment method for the porcine epithelial cell oxidative stress model comprises the following steps: inducing porcine epithelial cells by AAPH to continuously generate stable endogenous free radicals, detecting the oxidative damage degree of the cells, and determining a stress model of oxidative damage. According to the porcine epithelial cell oxidative stress model, the optimal concentration for constructingthe porcine small intestinal epithelial cell oxidative stress model induced by the AAPH is determined by a series of tests, and the oxidative damage is evaluated by the established oxidative stress model, so that the convenient and stable intestinal epithelial cell model is provided for further research of the influence of the oxidative stress on animal intestinal health, and the model has important significance for maintaining human health and reducing direct losses and indirect losses caused by oxidative stress in animal husbandry production.

Description

technical field [0001] The invention belongs to the technical field of cell model establishment, and in particular relates to a porcine epithelial cell oxidative stress model and its establishment method and application. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry industry, the problem of stress response in livestock and poultry has become increasingly serious. A large number of studies have shown that the stress of livestock and poultry can cause a variety of diseases, which seriously restricts the development of the breeding industry. During the breeding process, many factors, such as environmental changes, nutrient deficiency, trace element deficiency, high intake of polyunsaturated fatty acids, and the use of chemicals, may cause oxidative stress (oxidative stress, OS) in livestock and poultry. In the state of oxidative stress, the dynamic balance between the production and scavenging mechanism of oxygen fre...

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Application Information

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IPC IPC(8): C12N5/071C12Q1/02
CPCC12N5/0625G01N33/5044G01N33/502G01N2500/10G01N2800/7009
Inventor 鲁慧杰马现永陈卫东田志梅容庭刘志昌李贞明吕晓慧张莹
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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