Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection reagent for fluorescently labeling amino compounds and application of detection reagent in protein fluorescence labeling

An amino compound and protein fluorescence technology, which is applied in the field of detection reagents for fluorescently labeled amino compounds, can solve the problems of protein fluorescent labeling time increase, poor stability of labeled products, low fluorescence quantum yield, etc., and achieve high labeling efficiency and fast labeling speed , The effect of simple synthetic route

Pending Publication Date: 2020-11-24
INSPECTION & QUARANTINE TECH CENT OF NINGBO ENTRY EXIT INSPECTION & QUARANTINE BUREAU +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing protein fluorescent labeling reagents have the following disadvantages: 1. The labeling product has poor stability; 2. The labeling efficiency is low; 3. The fluorescence quantum yield is low; 4. It is necessary to use an organic solvent that can denature the protein to make the protein fluorescent The labeling time increases, and the reaction conditions are harsh when labeling

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection reagent for fluorescently labeling amino compounds and application of detection reagent in protein fluorescence labeling
  • Detection reagent for fluorescently labeling amino compounds and application of detection reagent in protein fluorescence labeling
  • Detection reagent for fluorescently labeling amino compounds and application of detection reagent in protein fluorescence labeling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A detection reagent for fluorescently labeled amino compounds, the structural formula of which is as follows:

[0040]

[0041] The preparation method of above-mentioned detection reagent is:

[0042] (1) Synthesis of fluorescein methyl ester:

[0043] (1.1) Dissolve fluorescein (3.32g, 10mmol) in 10ml of methanol, drop into 2.5ml of concentrated sulfuric acid, heat to reflux for 14 hours, and use Molecular sieve drying. After the above reaction stopped, add 1g ice, 10g NaHCO3 powder, filter, wash with a small amount of water, the red solid obtained is put into 50ml NaHCO3 aqueous solution with a mass fraction of 2%, filter again, and wash with water again. The water washing process was repeated three times.

[0044] (1.2) After washing with water, add 1% acetic acid solution, filter, and wash with water.

[0045] (1.3) The solid (red solid) obtained by the reaction was washed with water and then dried to obtain 3.4 g (98%) of fluorescein methyl ester as a red s...

Embodiment 2

[0052] A detection reagent for fluorescently labeled amino compounds, the structural formula of which is as follows:

[0053]

[0054]Its preparation method is basically the same as the preparation method of the detection reagent in Example 1, except that step (1) is not carried out, and it is directly synthesized from step (2).

Embodiment 3

[0056] A detection reagent for fluorescently labeled amino compounds, the structural formula of which is as follows:

[0057]

[0058] Its preparation method is the same as that of the detection reagent in Example 1, except that ethanol is selected as the solvent in step (1.1).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of protein fluorescence labeling, and particularly relates to a detection reagent for fluorescently labeling amino compounds and application of the detection reagentin protein fluorescence labeling. The detection reagent is shown as a structural general formula (1), and in the general formula (1), R represents-OH, -OCH3, -OCH2CH3, -OCH(CH3)2 and -OC(CH3)3. The detection reagent provided by the invention has the advantages of good marking product stability, high marking efficiency, high fluorescence quantum yield, short marking time and simple reaction conditions during marking.

Description

technical field [0001] The invention belongs to the field of protein fluorescent labeling, in particular to a detection reagent for fluorescently labeling amino compounds and its application in protein fluorescent labeling. Background technique [0002] As an important part of human cells, protein is the material basis of all life. The research on protein and proteomics is one of the hottest research directions in the field of scientific research, and plays an important role in medicine, life science and food safety testing. The detection of protein is the basis of protein research. The detection signal of the protein itself mainly comes from the ultraviolet absorption of the protein itself, and some proteins also have weak fluorescence. However, the intensity of these detection signals is weak, which makes the detection sensitivity low. signal, it is difficult to achieve effective detection. [0003] In order to make up for this deficiency, it is necessary to stain or la...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D405/12C09K11/06G01N21/64G01N33/533G01N33/58G01N33/68
CPCC07D405/12C09K11/06G01N21/6428G01N33/582G01N33/68G01N33/533C09K2211/1088C09K2211/1029
Inventor 李锦花马明冯睿肖道清石守江
Owner INSPECTION & QUARANTINE TECH CENT OF NINGBO ENTRY EXIT INSPECTION & QUARANTINE BUREAU