The invention discloses a preparation method of
DNA functionalized
quantum dots based on
click chemistry and application of the
DNA functionalized
quantum dots in biomarker and detection, and relates to the fields of nano materials and biomedical
analytical chemistry. According to the preparation method, in the synthesis process of
quantum dots,
DNA, of which one end is modified by
azide or diphenyl cyclooctyne and the other end is modified by
thiophosphate, is added to serve as a co-stabilizer of the quantum dots, and therefore, the DNA functionalized
quantum dot marking
reagent modified by
azide or diphenyl cyclooctyne is obtained. The
quantum dot marking
reagent can be used for efficiently marking
protease and
cancer cells and can be used for biological detection and
imaging analysis. On the basis of
a DNA hybridization principle, the marking
reagent also has important application values in the aspects of nano material self-
assembly and the like. Meanwhile, compared with other
fluorescence labeling reagents based on
click chemistry, the preparation method of the marking reagent is simple, no
chemical modification is needed, purification is easy, and the
fluorescence performance of the labeling reagent is excellent.