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Carbon quantum dot sensor with copper ion and cysteine recognition functions, preparation method and application thereof

A technology of carbon quantum dots and cysteine, which is applied in the application field of carbon quantum dot sensors and their preparation, and detection of cysteine, which can solve the problems of poor solubility, unfavorable, and limited sensor applications, and achieve high selectivity recognition and detection, convenient post-processing, and simple synthetic route

Inactive Publication Date: 2015-02-18
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the fluorescent sensors designed and synthesized based on the FRET principle involve relatively complicated synthetic routes, and most traditional fluorescent sensors have poor solubility in water and cannot be applied to pure water systems, which limits the use of such sensors in biological ion detection. And the application in the actual environment is not conducive to the application of scale-up synthesis and mass production

Method used

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  • Carbon quantum dot sensor with copper ion and cysteine recognition functions, preparation method and application thereof
  • Carbon quantum dot sensor with copper ion and cysteine recognition functions, preparation method and application thereof
  • Carbon quantum dot sensor with copper ion and cysteine recognition functions, preparation method and application thereof

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Embodiment 1

[0023] A preparation method of a carbon quantum dot sensor with dual recognition functions of copper ions and cysteine, comprising the following steps:

[0024] (1) Preparation of carbon quantum dots by microwave synthesis.

[0025] Place the mixed solution of 10g water, 1g citric acid, and 0.35g ethylenediamine on a microwave reactor with a power of 640W to react for 5 minutes to obtain a crude product, dissolve the crude product in 3ml water, precipitate in 40ml ethanol solution, and then vacuum dry , carbon quantum dots with carboxyl groups on the surface can be prepared.

[0026] (2) Surface functional modification of carbon quantum dots.

[0027] Dissolve 0.05g of carbon quantum dots containing carboxyl groups on the surface prepared in step (1) in 2.5ml of water, activate with 0.028g of NHS and 0.048g of EDC for 30min (pH-5~7), add 0.06g of 2-bromoethylamine to The above solution was stirred and reacted at room temperature for 2 days, and unreacted 2-bromoethylamine was ...

Embodiment 2

[0031] A preparation method of a carbon quantum dot sensor with dual recognition functions of copper ions and cysteine, comprising the following steps:

[0032] (1) Preparation of carbon quantum dots by microwave synthesis.

[0033] Place the mixed solution of 10g water, 1.5g citric acid, and 0.6g ethylenediamine on a microwave reactor with a power of 640W and react for 10 minutes to obtain a crude product. Dissolve the crude product in 5ml water, precipitate in 50ml ethanol solution, and vacuum After drying, carbon quantum dots with carboxyl groups on the surface can be prepared.

[0034] (2) Surface functional modification of carbon quantum dots.

[0035] Dissolve 0.05g of carbon quantum dots containing carboxyl groups on the surface prepared in step (1) in 2.0ml of water, activate with 0.025g of NHS and 0.045g of EDC for 30min (pH-5~7), add 0.09g of 2-bromoethylamine to The above solution was stirred and reacted at room temperature for 2 days, and unreacted 2-bromoethylam...

Embodiment 3

[0039] A preparation method of a carbon quantum dot sensor with dual recognition functions of copper ions and cysteine, comprising the following steps:

[0040] (1) Preparation of carbon quantum dots by microwave synthesis.

[0041] Place the mixed solution of 10g water, 1.2g citric acid, and 0.5g ethylenediamine on a microwave reactor with a power of 640W and react for 8 minutes to obtain a crude product. Dissolve the crude product in 4ml water, precipitate in 30ml ethanol solution, and vacuum After drying, carbon quantum dots with carboxyl groups on the surface can be prepared.

[0042] (2) Surface functional modification of carbon quantum dots.

[0043] Dissolve 0.05 g of carbon quantum dots containing carboxyl groups on the surface prepared in step (1) in 3.0 ml of water, activate with 0.03 g of NHS and 0.05 g of EDC for 30 min (pH-5~7), add 0.10 g of 2-bromoethylamine in The above solution was stirred and reacted at room temperature for 2 days, and unreacted 2-bromoethy...

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Abstract

The invention discloses a carbon quantum dot sensor with copper ion and cysteine recognition functions, a preparation method and application thereof. The carbon quantum dot sensor is prepared by the following steps: taking citric acid and ethylene diamine as raw materials, adopting a microwave method to synthesize a carbon quantum dot containing carboxyl on the surface, then taking 2-bromoethylamine and 1,4,8,11-tetraazacyclotetradecane as surface functionalizing reagents, and utilizing a surface grafting technology. The carbon quantum dot sensor is good in dispersity in water, and can be used for double-selectivity fluorescence detection to the copper ion and cysteine. Compared with the existing detection technology, the carbon quantum dot sensor disclosed by the invention can detect trace of copper ion and cysteine in a pure water medium with high sensitivity and high selectivity, is simple in synthetic route, convenient to use, and suitable for large-scale synthesis and actual application in production, and has the enormous application prospect in the fields such as biology and environment detection.

Description

technical field [0001] The invention relates to technical fields such as material preparation and ion detection in biology and the environment, and specifically relates to a carbon quantum dot sensor with dual recognition functions of copper ions and cysteine ​​and a preparation method thereof, and the carbon quantum dot sensor can be used in water Application of detection of copper ions and detection of cysteine ​​in organisms. Background technique [0002] Copper is an essential trace element for the human body and is widely distributed in biological tissues. Many electron transfer and redox reactions involving oxygen in biological systems are catalyzed by copper-containing enzymes, which are critical to life processes; copper levels in the body are critical for immune function, lipid and glucose metabolism, and hormones Secretion, etc. have certain effects; when the copper content in the body is too high, it will cause upper abdominal pain, nausea, vomiting, and even sho...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B31/02G01N21/64
Inventor 陈建李亚钟维邦吕锟王宏廖博易平贵
Owner HUNAN UNIV OF SCI & TECH
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