Carbon quantum dot sensor with copper ion and cysteine recognition functions, preparation method and application thereof

A technology of carbon quantum dots and cysteine, which is applied in the application field of carbon quantum dot sensors and their preparation, and detection of cysteine, which can solve the problems of poor solubility, unfavorable, and limited sensor applications, and achieve high selectivity recognition and detection, convenient post-processing, and simple synthetic route

Inactive Publication Date: 2015-02-18

HUNAN UNIV OF SCI & TECH

2 Cites 19 Cited by

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Problems solved by technology

However, most of the fluorescent sensors designed and synthesized based on the FRET principle involve relatively complicated synthetic routes, and most traditional fluorescent sensors have poor solubility in water and cannot...

Abstract

The invention discloses a carbon quantum dot sensor with copper ion and cysteine recognition functions, a preparation method and application thereof. The carbon quantum dot sensor is prepared by the following steps: taking citric acid and ethylene diamine as raw materials, adopting a microwave method to synthesize a carbon quantum dot containing carboxyl on the surface, then taking 2-bromoethylamine and 1,4,8,11-tetraazacyclotetradecane as surface functionalizing reagents, and utilizing a surface grafting technology. The carbon quantum dot sensor is good in dispersity in water, and can be used for double-selectivity fluorescence detection to the copper ion and cysteine. Compared with the existing detection technology, the carbon quantum dot sensor disclosed by the invention can detect trace of copper ion and cysteine in a pure water medium with high sensitivity and high selectivity, is simple in synthetic route, convenient to use, and suitable for large-scale synthesis and actual application in production, and has the enormous application prospect in the fields such as biology and environment detection.

Application Domain

Fluorescence/phosphorescenceLuminescent compositions

Technology Topic

BromineIon +16

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Experimental program(5)

Example Embodiment

[0022] Example 1:

[0023] A preparation method of a carbon quantum dot sensor with dual recognition functions of copper ion and cysteine, including the following steps:

[0024] (1) Preparation of carbon quantum dots by microwave synthesis.

[0025] Place a mixed solution of 10g water, 1g citric acid, and 0.35g ethylenediamine on a microwave reactor with a power of 640W to react for 5 minutes to obtain a crude product. The crude product is dissolved in 3ml water, precipitated in 40ml ethanol solution, and dried under vacuum. , Can prepare carbon quantum dots with carboxyl groups on the surface.

[0026] (2) Surface functional modification of carbon quantum dots.

[0027] Dissolve 0.05 g of carbon quantum dots containing carboxyl groups on the surface prepared in step (1) in 2.5 ml of water, activate with 0.028 g NHS and 0.048 g EDC for 30 minutes (pH-5~7), add 0.06 g of 2-bromoethylamine in The above solution was stirred and reacted at room temperature for 2 days. After 3 times of dialysis to remove unreacted 2-bromoethylamine, a carbon quantum dot solution functionalized with 2-bromoethylamine was obtained.

[0028] (3) Synthesis of 1,4,8,11-tetraazacyclotetradecane functionalized carbon quantum dots.

[0029] Weigh 0.2g of surface functionalizing reagent 1,4,8,11-tetraazacyclotetradecane and add it to the 2-bromoethylamine functionalized carbon quantum dot solution prepared in step (2), and stir to react at room temperature After 7 days of dialysis three times, the required carbon quantum dot sensor was prepared.

Example Embodiment

[0030] Example 2:

[0031] A preparation method of a carbon quantum dot sensor with dual recognition functions of copper ion and cysteine, including the following steps:

[0032] (1) Preparation of carbon quantum dots by microwave synthesis.

[0033] Place a mixed solution of 10g water, 1.5g citric acid, and 0.6g ethylenediamine on a microwave reactor with a power of 640W to react for 10 minutes to obtain a crude product. Dissolve the crude product in 5ml water, precipitate in 50ml ethanol solution, and then vacuum After drying, carbon quantum dots with carboxyl groups on the surface can be prepared.

[0034] (2) Surface functional modification of carbon quantum dots.

[0035] Dissolve 0.05g of carbon quantum dots containing carboxyl groups on the surface prepared in step (1) in 2.0ml of water, activate with 0.025g NHS and 0.045g EDC for 30 minutes (pH-5~7), add 0.09g of 2-bromoethylamine to The above solution was stirred and reacted at room temperature for 2 days. After 3 times of dialysis to remove unreacted 2-bromoethylamine, a carbon quantum dot solution functionalized with 2-bromoethylamine was obtained.

[0036] (3) Synthesis of 1,4,8,11-tetraazacyclotetradecane functionalized carbon quantum dots.

[0037] Weigh 0.18g surface functionalization reagent 1,4,8,11-tetraazacyclotetradecane and add it to the 2-bromoethylamine functionalized carbon quantum dot solution prepared in step (2), and stir to react at room temperature After 7 days of dialysis three times, the required carbon quantum dot sensor was prepared.

Example Embodiment

[0038] Example 3:

[0039] A preparation method of a carbon quantum dot sensor with dual recognition functions of copper ion and cysteine, including the following steps:

[0040] (1) Preparation of carbon quantum dots by microwave synthesis.

[0041] Place a mixed solution of 10g water, 1.2g citric acid, and 0.5g ethylenediamine on a microwave reactor with a power of 640W to react for 8 minutes to obtain a crude product. The crude product is dissolved in 4ml water and precipitated in 30ml ethanol solution. After drying, carbon quantum dots with carboxyl groups on the surface can be prepared.

[0042] (2) Surface functional modification of carbon quantum dots.

[0043] Dissolve 0.05 g of carbon quantum dots containing carboxyl groups on the surface prepared in step (1) in 3.0 ml of water, activate it with 0.03 g NHS and 0.05 g EDC for 30 min (pH-5~7), add 0.10 g of 2-bromoethylamine to The above solution was stirred and reacted at room temperature for 2 days. After 3 times of dialysis to remove unreacted 2-bromoethylamine, a carbon quantum dot solution functionalized with 2-bromoethylamine was obtained.

[0044] (3) Synthesis of 1,4,8,11-tetraazacyclotetradecane functionalized carbon quantum dots.

[0045] Weigh 0.25g of surface functionalization reagent 1,4,8,11-tetraazacyclotetradecane and add it to the 2-bromoethylamine functionalized carbon quantum dot solution prepared in step (2), and stir to react at room temperature After 7 days of dialysis three times, the required carbon quantum dot sensor was prepared.

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Classification and recommendation of technical efficacy words

The synthetic route is simple

Naked eye visual colorimetric method-based probe for detecting nickel ions and preparation method and applications thereof

InactiveCN102519949AThe synthetic route is simplelow cost

Owner:JIANGNAN UNIV

Carbon quantum dot sensor with copper ion and cysteine recognition functions, preparation method and application thereof

AI-Extracted Technical Summary

Problems solved by technology

Abstract

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Examples

Example Embodiment

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Example Embodiment

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Description & Claims & Application Information

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