Compound microbial agent for deodorizing livestock and poultry manure and preparation method of compound microbial agent

A compound microbial agent, livestock and poultry feces technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of secondary pollution removal costs and low removal efficiency, and achieve improved immunity and practical Strong and reasonable compound effect

Pending Publication Date: 2020-11-24
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods all have the problems of low removal efficiency, easy generation of secondary pollution and high removal cost.

Method used

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  • Compound microbial agent for deodorizing livestock and poultry manure and preparation method of compound microbial agent
  • Compound microbial agent for deodorizing livestock and poultry manure and preparation method of compound microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] To prepare a compound microbial bacterial agent for deodorizing livestock and poultry manure, the specific steps are as follows:

[0029] 1. Preparation of Seed Solution

[0030] Take the slant of Pseudomonas mutans, Brevibacterium cold-tolerant, Bacillus veilei, and Phyllostachys spp., and pick part of the bacterial lawn in a sterile environment and inoculate them in the liquid culture medium of beef extract peptone broth. Shaking culture was carried out at 30°C and 180 r / min for 24 hours. Take the slant of Lactobacillus rhamnosus, pick a part of the lawn and inoculate it into the MRS medium in a sterile environment, and culture it in an anaerobic incubator at 37°C for 24 hours. Obtain first-class seed liquid

[0031] 2. Seed liquid expansion culture

[0032] Inoculate the cultured Pseudomonas mutans, Brevibacterium cold-resistant, Bacillus velesi, Bamboobacterium, and Lactobacillus rhamnosus test tube primary seed solution under sterile conditions, respectively, in...

Embodiment 2

[0036] The composite deodorant bacterial agent obtained in Example 1 is carried out to detect the number of viable bacteria. The detection of the number of viable bacteria in the compound deodorant bacteria agent adopts the gradient dilution coating selective plate method. Nutrient gravy medium is used for the total colony count. Pseudomonas mutans and Brevibacterium cold-resistant belong to digestive bacteria. Heterotrophic nitrification medium with ammonium ion as the only nitrogen source is used. Bacillus velei and Stick bacteria belong to sulfur-removing bacteria Sulfur-containing inorganic medium is used, and MRS medium is used for lactic acid bacteria. The results are shown in the table below.

[0037] Total number of colonies Pseudomonas mutans Brevibacterium cold-tolerant Bacillus Velez stick bacteria Lactobacillus rhamnosus The number of viable bacteria (CFU / mL) 8.9×10 9

[0038] The results showed that the number of effective viable bact...

Embodiment 3

[0040] Detect the deodorizing effect of the composite deodorant bacterium agent obtained in embodiment 1, take the foul smell in the cow dung composting process as test object, by to NH 3 and H 2 The degree of reduction of the concentration of S harmful gas is measured to evaluate the deodorizing effect of the compound microbial deodorant agent.

[0041] The test site is arranged in a dairy farm in Zhangye City, Gansu Province, with more than 5,000 dairy cows in existence. The deodorization equipment is composed of deodorization pool, air pump, automatic control system, ammonia and hydrogen sulfide detector, etc.

[0042] Dilute the stock solution of the liquid compound deodorant bacteria agent 100 times as the bacteria agent used in the experiment, and directly add it to the deodorization pool. The deodorizing bacteria agent accounts for 1 / 3 of the volume of the deodorizing pool, and the odor is introduced from the lower part of the deodorizing pool with a flow rate of 10 cub...

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Abstract

The invention discloses a compound microbial agent for deodorizing livestock and poultry manure and a preparation method of the compound microbial agent. The compound microbial agent contains 12-20% of Pseudomonas plecoglossicida, 15-23% of Brevibacterium frigoritolerans, 30-35% of Bacillus velezensis strain, 18-22% of Arthrobacter bambusae and 5-10% of Lactobacillus rhamnosus. The compound microbial agent provided by the invention has the advantages of high treatment efficiency on odor in livestock and poultry manure, no secondary pollution, simple and convenient use method, low cost and widedevelopment prospect.

Description

technical field [0001] The invention relates to the field of microbial environment treatment, in particular to a compound microbial bacterial agent for deodorizing livestock and poultry manure and a preparation method thereof. Background technique [0002] The livestock and poultry breeding industry is an important part of my country's agriculture and rural economy, and it is also one of the main causes of rural pollution in our country. A large amount of livestock and poultry manure will ferment and produce various malodorous substances during the stacking process, including NH 3 、H 2 S, indole, methyl mercaptan, etc. Long-term exposure will cause harm to the health of humans and animals, and there is a potential for chronic poisoning. Therefore, accelerating compost maturity and reducing the emission of malodorous gases such as ammonia and hydrogen sulfide are one of the research hotspots to solve the problem of compost deodorization. [0003] At present, the deodoriza...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/02C02F103/20C12R1/38C12R1/13C12R1/07C12R1/06C12R1/225
CPCC12N1/20C02F11/02C02F2103/20C02F2303/02
Inventor 牛永艳朱瑞清王治业毛婷叶泽
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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