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Construction method of mouse Staphylococcus aureus mastitis model

A staphylococcus, construction method technology, applied in biochemical equipment and methods, microbial determination/inspection, animal husbandry, etc., can solve problems such as infection, anesthetic effects, etc., to avoid other infections, cost savings, modeling stable results

Active Publication Date: 2022-07-15
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, anesthesia before the injection of the bacterial solution may cause the anesthesia to affect the mice themselves and even the entire experiment; the method of injecting the bacterial solution after the mammary incision of the mouse and then suturing may lead to the risk of infection, resulting in the occurrence of an infection not caused by the injected bacterial solution. mastitis caused by

Method used

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  • Construction method of mouse Staphylococcus aureus mastitis model
  • Construction method of mouse Staphylococcus aureus mastitis model
  • Construction method of mouse Staphylococcus aureus mastitis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Construction of mouse mastitis model

[0051] The Staphylococcus aureus used in the present invention is isolated from the milk samples of clinical dairy cow mastitis, and is purified and cultivated by the laboratory itself; the ICR test mice are provided by the animal room of the School of Veterinary Medicine of Yangzhou University.

[0052] The present embodiment provides a method for constructing a mouse mastitis model using Staphylococcus aureus, comprising the following steps:

[0053] (1) Cultivation and enumeration of Staphylococcus aureus: Purify and culture the cryopreserved Staphylococcus aureus after recovery. Multi-fold dilutions were made with NaCl solution and colony counts were performed.

[0054] (2) According to the colony count result, dilute the concentration of Staphylococcus aureus to 1×10 with NaCl solution 4 CFU / mL.

[0055] (3) Six ICR mice with similar body size and at the expected date of birth were randomly divided into control gr...

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Abstract

The invention belongs to the technical field of molecular biology, and relates to a method for constructing a mouse Staphylococcus aureus mastitis model. In the present invention, the mouse mastitis model is constructed by injecting the Staphylococcus aureus suspension into the mouse mammary gland by using a micro-injector. The present invention utilizes a mouse mastitis model constructed by Staphylococcus aureus isolated from milk samples suffering from clinical dairy cow mastitis, and can be widely used in researches such as the prevention and treatment of dairy cow mastitis induced by Staphylococcus aureus, and the like. It provides experimental model basis for the study of the etiology, pathological mechanism, immune mechanism, pharmacokinetics and clinical prevention and treatment of dairy cow mastitis.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a method for constructing a mouse Staphylococcus aureus mastitis model. Background technique [0002] Cow mastitis is a mammary gland disease with high morbidity and high economic losses. It not only affects the production and quality of milk, but also endangers human health. The incidence of dairy cow mastitis is as high as 20%-30%. About 1 / 3 of dairy cows in the world suffer from different types of mastitis, which not only seriously affects the quality of milk, but also affects the reproduction, growth, development and milk production of dairy cows. The loss caused by mastitis in my country is more than 1200-3600 yuan / head, plus the loss of unsaleable milk and the cost of drugs for the treatment of cow mastitis, which has caused huge economic losses to the dairy farming industry and severely restricted the development of the dairy farming industry. . [0003] Pathogen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6851A01K67/02
CPCA01K67/02C12Q1/6851A01K2227/105A01K2267/0368C12Q2531/113C12Q2563/107
Inventor 杨章平周静鹏陈志曹翔梁艳
Owner YANGZHOU UNIV