Preparation method and an application of multi-vesicle type dihydromyricetin liposome

A technology of dihydromyricetin lipid and dihydromyricetin, which is applied in the field of preparation of multivesicular dihydromyricetin liposomes, can solve the problems of public health safety burden and decreased efficacy of antibiotics

Pending Publication Date: 2020-11-27
ZHONGKAI UNIV OF AGRI & ENG
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Problems solved by technology

[0002] Bacterial infectious diseases have created a serious burden on global public health security, and wit

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  • Preparation method and an application of multi-vesicle type dihydromyricetin liposome
  • Preparation method and an application of multi-vesicle type dihydromyricetin liposome
  • Preparation method and an application of multi-vesicle type dihydromyricetin liposome

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[0039] Vine tea is a traditional Chinese herbal medicine plant, which is widely grown in southern China. According to the records of "Chinese Herbal Medicine", Vine tea has various functions such as clearing away heat and dampness, calming the liver and lowering blood pressure, promoting blood circulation and dredging collaterals; modern clinical experiments have proved that Vine tea Rich in a polyphenolic flavonoid called dihydromyricetin (Dihydromyricetin, DMY), the content of its tender stems and leaves is as high as 30%. Many biological activities of dihydromyricetin have been confirmed, such as anti- Cancer, anti-inflammation, anti-alcoholism, liver protection and cardioprotection, etc. Biological experiments show that dihydromyricetin has strong antibacterial activity on S.aureus, and dihydromyricetin can interact with S.aureus cell membrane Membrane fats and proteins are found to interact, leading to changes in their conformation, disrupting the fluidity of cell membrane...

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Abstract

The invention relates to the technical field of dihydromyricetin, in particular to a preparation method and an application of a multi-vesicle type dihydromyricetin liposome. The preparation method comprises the following steps of (1) collecting vine tea stems and leaves to ampelopsis grossedentata powder, and performing water bath treatment, concentrating, filtering and cooling to dihydromyricetinextracted ingredients; (2) weighing the extracted ingredients, performing dissolving to obtain a dihydromyricetin solution, and detecting purity; (3) preparing an aqueous phase solution from Tween 80and PEG-4000, preparing an oil phase solution from the dihydromyricetin, cholesterol and egg yolk lecithin, and dropwise adding the oil phase solution to the aqueous phase solution, to prepare the multi-vesicle type dihydromyricetin liposome; and (4) performing centrifugation on the taken multi-vesicle type dihydromyricetin liposome solution, taking supernatant, and detecting absorbance. For extraction of the dihydromyricetin, the egg yolk lecithin is used as a liposome formwork, and macrogol 4000 is used as a modification wall material to prepare the multi-vesicle type dihydromyricetin liposome, so that the problems that the degree of hydrolysis of the dihydromyricetin is low, the biological half-life is short, and the film penetrating force is poor, are solved.

Description

technical field [0001] The invention relates to the technical field of dihydromyricetin, in particular to a preparation method and application of multivesicular dihydromyricetin liposomes. Background technique [0002] Bacterial infectious diseases have caused a serious burden on global public health security, and with the increase of bacterial resistance, the efficacy of more and more antibiotics has decreased significantly. Superbug outbreaks have led to massive human deaths and global panic, especially in developing countries with poor sanitation. According to the World Health Organization, antibiotic resistance may kill about 700,000 people worldwide each year. Some scholars predict that if no efforts are made to reduce bacterial resistance or develop new antibiotics, this number will reach 10 million people by 2050. Staphylococcus aureus (S.aureus) is one of the most common human pathogens, Widely present in various tissues of the human body, from surface such as skin ...

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Application Information

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IPC IPC(8): A61K9/127A61K31/353A61K36/87G01N21/33G01N1/38G01N1/34A61P31/04A61K127/00A61K135/00
CPCA61K31/353A61K36/87A61K9/1277A61K9/1271A61P31/04G01N21/33G01N1/38G01N1/34
Inventor 舒绪刚田允波罗帆黄运茂吴紫倩杨远廷胡洪超
Owner ZHONGKAI UNIV OF AGRI & ENG
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