Facial skin surface microorganism sampling method based on metagenomics and application

Abstract

The invention relates to a facial skin surface microorganism sampling method based on metagenomics. The method comprises the following steps: (1) dipping a disposable medical disinfection cotton swabinto sterile normal saline, and performing sampling in an area specified by a disposable sterilization specification plate; (2) in a sterile room, collecting samples according to the sequence of leftcheek, right cheek and forehead of a subject, and circling the cotton swabs obtained in the step (1) for 50 times in each area; and (3) putting the sampled cotton swab obtained in the step (2) into asterile Pbw solution, and performing storing at 4 DEG C for later use. The skin surface of the subject is not damaged in the sampling process, skin irritation caused by invasive sampling is avoided, safety is higher, and the method is suitable for facial skin sampling. Subsequent microbial community sequencing verifies that compared with other skin microbial sampling methods, the method provided by the invention can better restore the real micro-ecology of the skin.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a metagenomics-based microbial sampling method and application on the surface of facial skin. Background technique [0002] Human skin colonizes a variety of microorganisms and maintains a dynamic balance to maintain its unique skin microecology. Metagenome is the research object of all microbial genomes in the environment. Through high-throughput sequencing of the whole genome DNA in environmental samples, the saturated data volume of a single sample is obtained. Based on denovo assembly, microbial community structure diversity, microbial The composition and function of population genes, and the analysis of metabolic pathways related to specific environments, so as to further explore and study the genes with application value and the relationship between the microbial community in the environment and the relationship between microorganisms and the environment. [0003] A...

AI Technical Summary

Problems solved by technology

[0003] At present, most of the analysis of human microbial community characteristics is designed around the study of intestinal microorganisms. The structure of the skin and the intestinal tract is very different. The wrong skin sampling method will lead to sample contamination, low microbial biomass, and increase the qualitative and quantitative of microorganisms. Bias, creating barriers for microbiological research

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

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