Facial skin surface microorganism sampling method based on metagenomics and application

A microbial sampling and metagenomics technology, applied in the field of microbial sampling on the facial skin surface, can solve the problems of low microbial biomass, sample contamination, and increase the deviation of microbial qualitative and quantitative, so as to achieve cheap and easy-to-obtain materials, avoid Stimulation, high safety effect

Pending Publication Date: 2020-11-27
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0003] At present, most of the analysis of human microbial community characteristics is designed around the study of intestinal microorganisms. The structure of the skin and the intestinal tract is very different. The wrong skin sampling method will lead to sample contamin

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  • Facial skin surface microorganism sampling method based on metagenomics and application
  • Facial skin surface microorganism sampling method based on metagenomics and application
  • Facial skin surface microorganism sampling method based on metagenomics and application

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Embodiment Construction

[0027] Below in conjunction with embodiment, the present invention is further described, and following embodiment is descriptive, not limiting, can not limit protection scope of the present invention with following embodiment.

[0028] The raw materials used in the present invention, if no special instructions, are conventional commercially available products, the methods used in the present invention, if no special instructions, are conventional methods in this area, and the quality of each material used in the present invention is routine use quality.

[0029] A method for sampling microorganisms on the face skin surface based on metagenomics, the steps are as follows:

[0030] (1) Use a disposable medical sterile cotton swab dipped in sterile saline, and take a sample in the area specified by the disposable sterile specification plate;

[0031] (2) In the sterile room, collect samples in the order of the subject's left cheek, right cheek and forehead, and take the cotton s...

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Abstract

The invention relates to a facial skin surface microorganism sampling method based on metagenomics. The method comprises the following steps: (1) dipping a disposable medical disinfection cotton swabinto sterile normal saline, and performing sampling in an area specified by a disposable sterilization specification plate; (2) in a sterile room, collecting samples according to the sequence of leftcheek, right cheek and forehead of a subject, and circling the cotton swabs obtained in the step (1) for 50 times in each area; and (3) putting the sampled cotton swab obtained in the step (2) into asterile Pbw solution, and performing storing at 4 DEG C for later use. The skin surface of the subject is not damaged in the sampling process, skin irritation caused by invasive sampling is avoided, safety is higher, and the method is suitable for facial skin sampling. Subsequent microbial community sequencing verifies that compared with other skin microbial sampling methods, the method provided by the invention can better restore the real micro-ecology of the skin.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a metagenomics-based microbial sampling method and application on the surface of facial skin. Background technique [0002] Human skin colonizes a variety of microorganisms and maintains a dynamic balance to maintain its unique skin microecology. Metagenome is the research object of all microbial genomes in the environment. Through high-throughput sequencing of the whole genome DNA in environmental samples, the saturated data volume of a single sample is obtained. Based on denovo assembly, microbial community structure diversity, microbial The composition and function of population genes, and the analysis of metabolic pathways related to specific environments, so as to further explore and study the genes with application value and the relationship between the microbial community in the environment and the relationship between microorganisms and the environment. [0003] A...

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Application Information

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IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 赵化冰杨晨路福平何希宏史婷婷刘东青董萌杨柳王晓康
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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