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Preparation of risedronate zinc micro-nano adjuvant and application of adjuvant as vaccine adjuvant

A technology of zinc risedronate and risedronate, applied in the field of medicine, can solve the problems of unsatisfactory antigen immunity enhancement, poor tolerance, weak activity of aluminum adjuvant and the like

Pending Publication Date: 2020-12-01
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, aluminum adjuvants still have deficiencies: they can only induce Th2 type immune responses and humoral immunity, and have limited effects on inducing Th1 type immune responses and CTL responses; There is no adjuvant effect on virus, human immunodeficiency virus and other vaccines; compared with many new vaccine adjuvants, aluminum adjuvant has weak activity, and its immune enhancement effect on most genetically engineered antigens other than virus-like particle antigens is not ideal, etc.
In addition, small molecule agonists of soluble TLR7 / 8 tend to cause strong local and systemic toxicity in human trials, and are poorly tolerated

Method used

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  • Preparation of risedronate zinc micro-nano adjuvant and application of adjuvant as vaccine adjuvant
  • Preparation of risedronate zinc micro-nano adjuvant and application of adjuvant as vaccine adjuvant
  • Preparation of risedronate zinc micro-nano adjuvant and application of adjuvant as vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0085] Preparation Example 1: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 0.25))

[0086] Risedronate Sodium (C 7 h 10 NNaO 7 P 2 ): purchased from Hunan Huateng Pharmaceutical Co., Ltd.

[0087] Anhydrous Zinc Chloride (ZnCl 2 ): purchased from Xilong Chemical

[0088] Disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O): purchased from Xilong Chemical Industry

[0089] Sodium hydroxide (NaOH): purchased from Xilong Chemical Industry

[0090] Solution preparation:

[0091] According to the Zn / risedronic acid molar concentration ratio of 1:0.25, prepare 50mL of 31.11mM zinc chloride solution, defined as solution A; prepare 50mL (7.78mM risedronic acid+36mM sodium hydroxide+15.55mM dihydrogen phosphate Sodium) solution is defined as B solution, and A solution and B solution are filtered with a 0.22 μm filter membrane for subsequent use.

[0092] Preparation of Zn-risedronic acid (1 / 0.25) adjuvant suspension and determination of physic...

preparation example 2

[0095] Preparation Example 2: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 1))

[0096] Reagent sources refer to Preparation Example 1.

[0097] Solution preparation:

[0098] According to the Zn / risedronic acid molar concentration ratio of 1:1, prepare 50mL 31.11mM zinc chloride solution, which is defined as solution A; prepare 50mL (31.11mM risedronic acid + 60mM sodium hydroxide) solution, which is defined as B solution, solution A and solution B were filtered with a 0.22 μm filter membrane for later use.

[0099] Preparation of Zn-risedronic acid (1 / 1) adjuvant suspension and determination of physical and chemical properties:

[0100]Please refer to Preparation Example 1.

preparation example 3

[0101] Preparation Example 3: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 0.125))

[0102] Reagent sources refer to Preparation Example 1.

[0103] Solution preparation:

[0104] According to the Zn / risedronic acid molar concentration ratio of 1:0.125, prepare 1L of 124.44mM zinc chloride solution, which is defined as solution A; prepare 1L (15.56mM risedronic acid+60mM sodium hydroxide+184mM disodium hydrogen phosphate ) solution, which is defined as B solution, A solution and B solution are filtered with 0.22 μm filter membrane for later use.

[0105] Preparation of Zn-risedronic acid (1 / 0.125) adjuvant suspension and determination of physical and chemical properties:

[0106] Please refer to Preparation Example 1.

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Abstract

The invention belongs to the technical field of medicines. Specifically, the invention relates to a risedronate zinc micro-nano adjuvant which is formed by mineralizing zinc ions and risedronate as main components and has a slow release function and application of the adjuvant in a vaccine adjuvant. The invention also relates to a preparation method of the risedronic acid micro-nano adjuvant. Theinvention also relates to a chemical composition, the vaccine adjuvant and a vaccine composition containing the risedronic acid micro-nano adjuvant. The invention also relates to application of the risedronic acid micro-nano adjuvant as the vaccine adjuvant.

Description

technical field [0001] The invention belongs to the technical field of medicine. Specifically, the present invention relates to a zinc risedronate micro-nano adjuvant with a slow-release function formed through the mineralization of zinc ions and risedronic acid as the main components and its use as a vaccine adjuvant. The invention also relates to a preparation method of the risedronate zinc micro-nano adjuvant. The present invention also relates to the chemical composition, vaccine adjuvant and vaccine composition containing risedronate zinc micro-nano adjuvant. The invention also relates to the use of the risedronate zinc micronano adjuvant as a vaccine adjuvant. [0002] technical background [0003] Adjuvants are substances that can specifically or non-specifically bind to immunogens, induce the body to produce long-term and effective specific immune responses, and play an auxiliary role. The immunobiological effects of adjuvants include reducing the amount of immunog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/25A61K41/10A61P31/22B82Y5/00
CPCA61K39/39A61K39/12B82Y5/00A61P31/22A61K2039/55511C12N2710/16734A61P37/04A61K2039/55555A61P31/20A61K2039/55505C12N2730/10134A61K2039/575A61K31/675A61K33/30A61K2300/00A61K39/25A61K2039/55516
Inventor 赵勤俭黄小芬李宜珂聂梅凤陈思伊夏宁邵
Owner XIAMEN UNIV
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