Preparation of risedronate zinc micro-nano adjuvant and application of adjuvant as vaccine adjuvant

Abstract

The invention belongs to the technical field of medicines. Specifically, the invention relates to a risedronate zinc micro-nano adjuvant which is formed by mineralizing zinc ions and risedronate as main components and has a slow release function and application of the adjuvant in a vaccine adjuvant. The invention also relates to a preparation method of the risedronic acid micro-nano adjuvant. Theinvention also relates to a chemical composition, the vaccine adjuvant and a vaccine composition containing the risedronic acid micro-nano adjuvant. The invention also relates to application of the risedronic acid micro-nano adjuvant as the vaccine adjuvant.

Description

technical field

[0001] The invention belongs to the technical field of medicine. Specifically, the present invention relates to a zinc risedronate micro-nano adjuvant with a slow-release function formed through the mineralization of zinc ions and risedronic acid as the main components and its use as a vaccine adjuvant. The invention also relates to a preparation method of the risedronate zinc micro-nano adjuvant. The present invention also relates to the chemical composition, vaccine adjuvant and vaccine composition containing risedronate zinc micro-nano adjuvant. The invention also relates to the use of the risedronate zinc micronano adjuvant as a vaccine adjuvant.

[0002] technical background

[0003] Adjuvants are substances that can specifically or non-specifically bind to immunogens, induce the body to produce long-term and effective specific immune responses, and play an auxiliary role. The immunobiological effects of adjuvants include reducing the amount of immunog...

Examples

preparation example 1

[0085] Preparation Example 1: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 0.25))

[0086] Risedronate Sodium (C 7 h 10 NNaO 7 P 2 ): purchased from Hunan Huateng Pharmaceutical Co., Ltd.

[0087] Anhydrous Zinc Chloride (ZnCl 2 ): purchased from Xilong Chemical

[0088] Disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O): purchased from Xilong Chemical Industry

[0089] Sodium hydroxide (NaOH): purchased from Xilong Chemical Industry

[0090] Solution preparation:

[0091] According to the Zn / risedronic acid molar concentration ratio of 1:0.25, prepare 50mL of 31.11mM zinc chloride solution, defined as solution A; prepare 50mL (7.78mM risedronic acid+36mM sodium hydroxide+15.55mM dihydrogen phosphate Sodium) solution is defined as B solution, and A solution and B solution are filtered with a 0.22 μm filter membrane for subsequent use.

[0092] Preparation of Zn-risedronic acid (1 / 0.25) adjuvant suspension and determination of physic...

preparation example 2

[0095] Preparation Example 2: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 1))

[0096] Reagent sources refer to Preparation Example 1.

[0097] Solution preparation:

[0098] According to the Zn / risedronic acid molar concentration ratio of 1:1, prepare 50mL 31.11mM zinc chloride solution, which is defined as solution A; prepare 50mL (31.11mM risedronic acid + 60mM sodium hydroxide) solution, which is defined as B solution, solution A and solution B were filtered with a 0.22 μm filter membrane for later use.

[0099] Preparation of Zn-risedronic acid (1 / 1) adjuvant suspension and determination of physical and chemical properties:

[0100]Please refer to Preparation Example 1.

preparation example 3

[0101] Preparation Example 3: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 0.125))

[0102] Reagent sources refer to Preparation Example 1.

[0103] Solution preparation:

[0104] According to the Zn / risedronic acid molar concentration ratio of 1:0.125, prepare 1L of 124.44mM zinc chloride solution, which is defined as solution A; prepare 1L (15.56mM risedronic acid+60mM sodium hydroxide+184mM disodium hydrogen phosphate ) solution, which is defined as B solution, A solution and B solution are filtered with 0.22 μm filter membrane for later use.

[0105] Preparation of Zn-risedronic acid (1 / 0.125) adjuvant suspension and determination of physical and chemical properties:

[0106] Please refer to Preparation Example 1.

technical field

[0001] The invention belongs to the technical field of medicine. Specifically, the present invention relates to a zinc risedronate micro-nano adjuvant with a slow-release function formed through the mineralization of zinc ions and risedronic acid as the main components and its use as a vaccine adjuvant. The invention also relates to a preparation method of the risedronate zinc micro-nano adjuvant. The present invention also relates to the chemical composition, vaccine adjuvant and vaccine composition containing risedronate zinc micro-nano adjuvant. The invention also relates to the use of the risedronate zinc micronano adjuvant as a vaccine adjuvant.

[0002] technical background

[0003] Adjuvants are substances that can specifically or non-specifically bind to immunogens, induce the body to produce long-term and effective specific immune responses, and play an auxiliary role. The immunobiological effects of adjuvants include reducing the amount of immunog...

Examples

preparation example 1

[0085] Preparation Example 1: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 0.25))

[0086] Risedronate Sodium (C 7 h 10 NNaO 7 P 2 ): purchased from Hunan Huateng Pharmaceutical Co., Ltd.

[0087] Anhydrous Zinc Chloride (ZnCl 2 ): purchased from Xilong Chemical

[0088] Disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O): purchased from Xilong Chemical Industry

[0089] Sodium hydroxide (NaOH): purchased from Xilong Chemical Industry

[0090] Solution preparation:

[0091] According to the Zn / risedronic acid molar concentration ratio of 1:0.25, prepare 50mL of 31.11mM zinc chloride solution, defined as solution A; prepare 50mL (7.78mM risedronic acid+36mM sodium hydroxide+15.55mM dihydrogen phosphate Sodium) solution is defined as B solution, and A solution and B solution are filtered with a 0.22 μm filter membrane for subsequent use.

[0092] Preparation of Zn-risedronic acid (1 / 0.25) adjuvant suspension and determination of physic...

preparation example 2

[0095] Preparation Example 2: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 1))

[0096] Reagent sources refer to Preparation Example 1.

[0097] Solution preparation:

[0098] According to the Zn / risedronic acid molar concentration ratio of 1:1, prepare 50mL 31.11mM zinc chloride solution, which is defined as solution A; prepare 50mL (31.11mM risedronic acid + 60mM sodium hydroxide) solution, which is defined as B solution, solution A and solution B were filtered with a 0.22 μm filter membrane for later use.

[0099] Preparation of Zn-risedronic acid (1 / 1) adjuvant suspension and determination of physical and chemical properties:

[0100]Please refer to Preparation Example 1.

preparation example 3

[0101] Preparation Example 3: Preparation of Zinc Risedronate Adjuvant (Zn-Risedronic Acid (1 / 0.125))

[0102] Reagent sources refer to Preparation Example 1.

[0103] Solution preparation:

[0104] According to the Zn / risedronic acid molar concentration ratio of 1:0.125, prepare 1L of 124.44mM zinc chloride solution, which is defined as solution A; prepare 1L (15.56mM risedronic acid+60mM sodium hydroxide+184mM disodium hydrogen phosphate ) solution, which is defined as B solution, A solution and B solution are filtered with 0.22 μm filter membrane for later use.

[0105] Preparation of Zn-risedronic acid (1 / 0.125) adjuvant suspension and determination of physical and chemical properties:

[0106] Please refer to Preparation Example 1.

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