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Application of sennoside A in preparation of drugs for treating liver cancer

A technology for the treatment of liver cancer and sennoside, which is applied in the field of biomedicine and can solve problems such as poor efficacy

Inactive Publication Date: 2020-12-04
SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned technical problems in the prior art, the present invention provides the purposes of sennoside A in the preparation of the medicine for treating liver cancer, and described this use will solve the medicine in the prior art that the effect of the medicine for the treatment of liver cancer is not enough. good technical questions

Method used

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  • Application of sennoside A in preparation of drugs for treating liver cancer
  • Application of sennoside A in preparation of drugs for treating liver cancer
  • Application of sennoside A in preparation of drugs for treating liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The migration and invasion effects of sennoside A on two liver cancer cell lines were detected at the cell level.

[0034] 1. Experimental materials: human liver cancer cell line and its culture: HepG2 and SMMC-7721 cells were purchased from the cell bank of China Center for Type Culture Collection. Cells were cultured in DMEM-H (Gibco) medium containing 10% fetal bovine serum (Gibco) at 37°C, 5% CO 2 cultivated under conditions. Sennoside A was provided by Selleck Chemicals (Shanghai, China); unless otherwise noted, other reagents such as Trizol and DMSO were products of Sigma.

[0035]2. Experimental method: Transwell plates with 6.5mm transparent polycarbonate (PC) film and gel-coated Transwell plates with 6.5mm transparent polyester (PET) film were used respectively (article numbers 3422 and 354480 respectively; Corning-Costar , Cambridge, MA, USA) to detect cell migration and invasion abilities.

[0036] Specific steps are as follows:

[0037] (1) Pre...

Embodiment 2

[0044] Example 2 Screening of sennoside A anti-metastasis targets of hepatocellular carcinoma by transcriptomics technology.

[0045] Step 1. Experimental process:

[0046] 1. mRNA isolation: Deactivate the total RNA sample, denature at a suitable temperature to open its secondary structure, and use oligo(dT) magnetic beads to enrich mRNA.

[0047] 2. mRNA interruption: Add an interruption reagent to the mRNA obtained in the previous step, react at an appropriate temperature for a certain period of time, and fragment the mRNA.

[0048] 3. cDNA synthesis: Add the pre-prepared one-strand synthesis reaction system to the interrupted mRNA, synthesize the first-strand cDNA according to the corresponding procedures on the PCR instrument, prepare the second-strand synthesis reaction system, react at an appropriate temperature for a certain period of time, and synthesize the second strand cDNA.

[0049] 4. End repair and adapter ligation: Prepare a reaction system, react at an appro...

Embodiment 3

[0073] Example 3 Molecular biological technology verification of the anti-metastasis target of sennoside A in hepatocellular carcinoma.

[0074] Step 1. Gene level: use RT-PCR technology to verify the shared differential genes related to tumor metastasis in two different liver cancer cell lines screened out by transcriptomics technology.

[0075] 1. Total RNA extraction

[0076] (1) Total RNA was extracted using RNA extraction kit (Promega, Shanghai, China) according to the product instructions.

[0077] (2) Detection of RNA concentration and purity. Use Nano-drop 2000 to detect RNA concentration and purity. When A260 / A280 is 1.8-2.0, it indicates that the purity of RNA is high. Such as A260 / A2802.2, indicating that RNA is degraded into single nucleotides. In both cases RNA should be re-extracted. The RNA concentration is preferably below 500 ng / μl.

[0078] 2. Reverse transcription

[0079] The reaction system of the kit (TOYOBO Bio-Technology, Shanghai, China) is as f...

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Abstract

The invention provides application of sennoside A in preparation of drugs for treating liver cancer. The invention also provides application of serine protease inhibitor superfamily protein 2 as a target for screening drugs for treating liver cancer. The invention also provides application of WNT signal pathway inhibitor 1 as a target for screening drugs for treating liver cancer. The invention also provides application of keratin KRT7 as a target for screening drugs for treating liver cancer. The invention also provides application of keratin KRT81 as a target for screening drugs for treatingliver cancer. The invention proves that the sennoside A has the effect of treating liver cancer, and successfully screens and verifies the target and related pathway of sennoside SA for resisting hepatocellular carcinoma metastasis.

Description

Technical field: [0001] The invention belongs to the field of biomedicine and relates to sennoside A, especially the use of sennoside A in the preparation of medicines for treating liver cancer. Background technique: [0002] The latest cancer epidemiological data show that primary liver cancer is the sixth most common cancer and the fourth leading cause of cancer death worldwide. Hepatocellular Carcinoma (HCC) is the most common type of primary liver cancer in most countries, accounting for about 75%-85% of the total cases. A variety of treatments have been used to treat HCC in the past, including surgical resection, chemotherapy, local radiotherapy, immunotherapy, and systemic therapy. However, due to postoperative recurrence and drug resistance of HCC, the curative effect is still unsatisfactory. In addition, postoperative recurrence and metastasis rates of HCC are extremely high. In recent years, many traditional Chinese medicines have been studied as alternative chem...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P35/00C12Q1/6886G01N33/574
CPCA61K31/704A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158G01N33/57438G01N33/57484G01N2333/4704G01N2333/4742G01N2333/8121G01N2500/04
Inventor 邬海龙乐佳美傅怡韩秋琴
Owner SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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