Spore production culture method for alternaria tenuissima of citrus reticulata blanco and application of method

Abstract

The invention belongs to the technical field of biology, and particularly relates to a spore production culture method for alternaria tenuissima of citrus reticulata blanco and an application of the method The alternaria tenuissima is taken as an object, and the culture time is studied; the culture temperature is controlled; low-temperature induction is conducted; illumination is conducted; a carbon source is added; a nitrogen source is added; and the influence of the culture medium variety on the mycelium growth and the sporulation quantity of thealternaria tenuissima is reduced, and then sporulation conditions are optimized in combination with an orthogonal Latin square test. The optimal spore production condition of pathogenic bacteria after optimization is 25 DEG C; under a full-dark culture condition, optimized PSY is used as a culture medium (12.0 g of maltose replaces 2.0 g of cane sugar in a PSY culture medium, 20.0 g of potassium nitrate replaces 10.0 g of peptone and 5.0 g ofyeast extract), 20-minute ultraviolet irradiation and 3-hour 15-DEG C induction are conducted on the pathogenic bacteria in a specific time period every day, and the sporulation quantity is measuredon the fourth day of culture.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a spore-producing culture method of citrus brown spot fungus and its application. Background technique [0002] Citrus (Citrus reticulata Blanco) is a Rutaceae plant, which is the general name of tangerine, tangerine, orange, kumquat, pomelo, and orange. The citrus pulp is sweet and sour, and is deeply loved by men, women and children, and is the second largest fruit in my country after apples. [0003] Citrus is easily affected by pests and diseases, and there are dozens of common diseases, such as citrus huanglongbing (Candidatus Liberibacter asiaticus), citrus scab (Sphacelomafawcetti), citrus anthracnose (Colletotrichum gloeosporioides) and so on. [0004] Citrus brown spot can cause a large number of citrus leaf and fruit drop, and even the branches die, which will have a great impact on the yield and quality of citrus. Since citrus brown spot was first discovered...

AI Technical Summary

Problems solved by technology

However, the amount of spore production of this pathogen is relatively small, which has formed certain difficulties for indoor research. Therefore, this experiment carried out biological characteristics research and spore production optimization experiments on Alternaria tenopolaris to provide a certain basis for the prevention and treatment of citrus brown spot.

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