Extraction and purification of glycyrrhiza uralensis submicron powder polysaccharide

A technology of licorice polysaccharides and powder polysaccharides, which is applied in the field of extraction and purification of licorice ultrafine powder polysaccharides, can solve the problem of less separation and purification, achieve good dispersion, promote dissolution and release, and reduce loss.

Active Publication Date: 2020-12-08
NANJING KANGQI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on licorice polysaccharides is mainly focused on extraction, but there are few reports on separation and purification. However, crystal gel medium biochromatography can effectively enrich biologically active substances and has high selectivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of extraction and purification of licorice superfine powder polysaccharide extraction, comprising the following steps:

[0022] S1. Weigh the licorice, wash and chop it, dry it at 50°C for 72 hours, and crush it through an 80-mesh sieve to obtain a licorice coarse powder, then process the coarse powder with a vibrating ultrafine pulverizer to obtain a 500-mesh licorice superfine powder, place Store in a desiccator for later use;

[0023] S2. Add licorice powder to distilled water, the ratio of solid to liquid is 1:30, ultrasonic power is 600W, and the duty ratio is 1:1 for ultrasonic-assisted extraction for 30min, after ultrasonication, let stand for 1h, and then rotate at a speed of 8000r·min -1 , and centrifuged at 4°C for 10 min to obtain the supernatant and precipitate, remove the supernatant and rotary evaporate until the volume is 1 / 10 of the original volume, first add 95% ethanol solution to the supernatant to make the final ethanol concentration of the sa...

Embodiment 2

[0029] A kind of extraction and purification of licorice superfine powder polysaccharide extraction, comprising the following steps:

[0030] S1. Weigh the licorice, wash and chop it, dry it at 50°C for 72 hours, and crush it through an 80-mesh sieve to obtain a licorice coarse powder, then process the coarse powder with a vibrating ultrafine pulverizer to obtain a 300-mesh licorice superfine powder, place Store in a desiccator for later use;

[0031] S2. Add licorice powder to distilled water, the ratio of solid to liquid is 1:50, ultrasonic power is 450W, and the duty ratio is 1:1 to carry out ultrasonic-assisted extraction for 30min, after ultrasonication, let stand for 1h, and then rotate at a speed of 10000r·min -1 , and centrifuged at 4°C for 10 min to obtain the supernatant and precipitate, remove the supernatant and rotary evaporate until the volume is 1 / 10 of the original volume, first add 95% ethanol solution to the supernatant to make the final ethanol concentration...

Embodiment 3

[0037] A kind of extraction and purification of licorice superfine powder polysaccharide extraction, comprising the following steps:

[0038] S1. Weigh the licorice, wash and chop it, dry it at 50°C for 72 hours, and crush it through an 80-mesh sieve to obtain a licorice coarse powder, then process the coarse powder with a vibrating ultrafine pulverizer to obtain a 300-mesh licorice superfine powder, place Store in a desiccator for later use;

[0039] S2. Add licorice powder to distilled water, the ratio of solid to liquid is 1:40, ultrasonic power is 600W, and the duty ratio is 1:1 for ultrasonic-assisted extraction for 30min. -1 , and centrifuged at 4°C for 10 min to obtain the supernatant and precipitate, remove the supernatant and rotary evaporate until the volume is 1 / 10 of the original volume, first add 95% ethanol solution to the supernatant to make the final ethanol concentration of the sample 30%, Stand still for 12 hours, centrifuge to obtain crude licorice polysacc...

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PUM

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Abstract

The invention provides extraction and purification of glycyrrhiza uralensis submicron powder polysaccharide. The extraction and purification method comprises the following steps: S1, preparing glycyrrhiza uralensis submicron powder; S2, preparing a glycyrrhiza polysaccharide crude product; S3, dissolving the glycyrrhiza polysaccharide crude product into distilled water, and passing through a 0.30-0.50 mu m microfiltration membrane; S4, diluting the glycyrrhiza polysaccharide solution, enabling the glycyrrhiza polysaccharide solution to pass through a DEAE-52 cellulose chromatographic column for three times, and freeze-drying to obtain purified glycyrrhiza polysaccharide. The extraction method is high in glycyrrhiza polysaccharide yield, and glycyrrhiza polysaccharide obtained through the purification method is high in purity.

Description

technical field [0001] The invention relates to the field of extraction and purification of chemical components, in particular to the extraction and purification of licorice superfine powder polysaccharides. Background technique [0002] Glycyrrhiza licorice is a perennial herbaceous plant belonging to the leguminous licorice family. It has high medicinal value and is sweet and flat in nature. Glycyrrhiza Polysaccharide, the main chemical component in licorice, has various biological activities such as immune regulation, enhancement of macrophage phagocytosis, and anti-oxidation. Glycyrrhizae crude polysaccharides can be separated and extracted by different methods, such as water extraction and alcohol precipitation method, ultrasonic-assisted method, microwave, enzymatic method and so on. However, in order to obtain licorice polysaccharide with higher purity, the crude extract needs to be further purified. At present, domestic research on licorice polysaccharides mainly f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 谈满良李小冬
Owner NANJING KANGQI BIOTECHNOLOGY CO LTD
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