New applications of Lpro protein and applications of FMDV L gene deletion mutant strain

A technology of protein mutants and proteins, applied in the field of virus immunology

Pending Publication Date: 2020-12-11
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The role of viral proteins in innate immunity remains to be elucidated in order to control and elimina...

Method used

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  • New applications of Lpro protein and applications of FMDV L gene deletion mutant strain
  • New applications of Lpro protein and applications of FMDV L gene deletion mutant strain
  • New applications of Lpro protein and applications of FMDV L gene deletion mutant strain

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Experimental program
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Embodiment

[0052] (1) Construction of the foot-and-mouth disease recombinant virus FMDV LbKO with Lb gene deletion

[0053] 1.1, the construction and rescue of the O-type foot-and-mouth disease recombinant virus of Lb gene deletion, comprise the following steps:

[0054] (1) primers are designed according to the sequence of prO-FMDV:

[0055] Primers for amplifying the L gene:

[0056] O1F: 5'-ATAGGTTTAATTAATGTTAAGCGTCTG-3' (SEQ ID NO: 2);

[0057] O1R: 5'-GTCTGTGGATCCCTCGTTGGAGCC-3' (SEQ ID NO: 3); the upstream primer O1F for amplifying the L gene contains a PacI restriction site, and the downstream primer O1R contains a BamHI restriction site.

[0058] Primers for deletion of the Lb gene on the L gene:

[0059] ODLbF: 5'-CACGCACCACAGGAAAAATGGGAGCCGGACAATCCAGTCC-3' (SEQ ID NO. 4);

[0060] ODLbR: 5'-GGACTGGATTGTCCGGCTCCCATTTTTCCTGTGGTGCGTG-3' (SEQ ID NO. 5);

[0061] (2) Using the plasmid prO-FMDV of the recombinant O-type foot-and-mouth disease virus rescue system as a template, u...

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Abstract

The present invention belongs to the field of virus immunology and particularly relates to new applications of Lpro protein and applications of an FMDV L gene deletion mutant strain. The present invention finds that the Lpro hinders JAK-STAT signal transduction in a dose-dependent manner. At the same time, a new function of the Lpro is discovered, i.e., intracellular components of a JAK-STAT pathway in cells are degraded in a lysosome-dependent manner through interaction with a specific region of STATs, rather than acting on the JAK-STAT signal transduction through classical protease activityof the Lpro. A protease activity center site of the Lpro of the FMDV is a key site participating in a lysosome degradation pathway. A protease activity center site of the Lpro is a key site of K48 ubiquitination of the Lpro. The new applications of the Lpro protein and the applications of the FMDV L gene deletion mutant strain provide new targets and theoretical supports for prevention and controland vaccine design of viruses of RNA viridae, such as viruses of foot-and-mouth diseases.

Description

technical field [0001] The invention belongs to the field of virus immunology, especially relates to L pro The application of protein in the preparation of JAK-STAT signal transduction inhibitor, and the protein L (comprising two kinds of shear mutants of Lab and Lb) of RNA virus family (Picornaviridae) foot-and-mouth disease virus (FMDV), EMCV virus and SAFV 51 Novel functions of cysteine ​​residues (protease active sites). Background technique [0002] Interferons (IFNs) are a group of secreted proteins, usually induced by pathogen-associated molecular patterns (PAMPs) activated by host pattern recognition receptors (PRRs), that play key roles in host antiviral innate and adaptive immunity. Interferon-based therapy has been successfully used in the clinical treatment of many viral infections, such as hepatitis B virus (HBV). To date, three types of IFNs have been identified to make up the entire IFN family, among which type I (IFNα / β) and type II IFN (IFN-γ) are regulate...

Claims

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Application Information

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IPC IPC(8): C07K14/085C07K14/09C12N15/41C12N15/42A61K39/125A61K39/135A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2770/32122C12N2770/32222C12N2770/32134C12N2770/32234A61K2039/5254A61K2039/552
Inventor 郑海学马旭升罗志宽杨帆宋锐曾宗波莫希丁·乔杜里曹伟军刘永杰张克山田宏毛若箐刘湘涛
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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