A kind of immobilized algae ball and its preparation method and application

A technology of algae balls and algae, which is applied in the field of immobilized algae balls and its preparation, can solve the problems of high mechanical strength of immobilized algae balls, easy decomposition of immobilized algae balls, and inability to store for a long time, so as to facilitate large-scale production, Good stability and activity, high stability effect

Active Publication Date: 2022-04-22
青岛鲜达物流科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the deficiencies of the above-mentioned technologies, and provide an immobilized algae ball and its preparation method and application. The immobilized algae ball prepared by the method has high mechanical strength, the microalgae is not easy to lose, the degradation efficiency is high, and it can be recycled. It solves the technical problems that the existing immobilized algae balls are easy to decompose, cannot be stored for a long time, and have low degradation efficiency.

Method used

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  • A kind of immobilized algae ball and its preparation method and application
  • A kind of immobilized algae ball and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cultivation of microbial algae liquid: inoculate Sclerophytum in BG11 medium, and cultivate for 8 days under the conditions of temperature 25-28°C, light intensity 5000±500Lx, and pH 6-7 to prepare microbial algae liquid.

Embodiment 2

[0033] Preparation of algae mixture: heat 8ml of acetyl tri-n-butyl citrate (ATBC) to 100°C and then cool down to 70-80°C for later use;

[0034] Add 2g of sodium alginate into 100ml of water, stir evenly at 60 to 70°C (sodium alginate is added in four times, 0.5g each time), sterilize with high temperature steam at 121°C for 20min, add chitosan 2g, stir well and then ultrasonically After ultrasonic treatment in the crusher for 1 min, add the cooled acetyl tri-n-butyl citrate, mix thoroughly and the temperature drops below 25°C.

[0035] Preparation of algae cross-linking agent: Add 25g of boric acid into 500ml of water, stir well to dissolve, add 5g of calcium chloride and continue to stir evenly, and adjust the pH to 7.0-7.4 with 2mol / L NAOH solution, and use it after high-temperature steam sterilization at 121°C.

[0036] Add the microbial algae solution prepared in Example 1 into the algae mixture at a volume ratio of 0.9:1, and stir evenly to obtain the embedding solution...

Embodiment 3

[0038] Preparation of algae mixture: heat 10ml of acetyl tri-n-butyl citrate (ATBC) to 100°C and then cool down to 70-80°C for later use;

[0039] Add 2.5g of sodium alginate into 100ml of water, stir evenly at 60 to 70°C (sodium alginate is added in four times), sterilize with high-temperature steam at 121°C for 20 minutes, add chitosan 3.5g, stir evenly, and place in an ultrasonic breaker After ultrasonic treatment for 1 min, add the cooled acetyl tri-n-butyl citrate, mix thoroughly and the temperature drops below 25°C.

[0040] Preparation of algae cross-linking agent: Add 25g of boric acid to 500ml of water, fully stir to dissolve, add 7.5g of calcium chloride and continue to stir evenly, and adjust the pH to 7.0-7.4 with 2mol / L NAOH solution, and use it after high-temperature steam sterilization at 121°C .

[0041] Add the microbial algae solution prepared in Example 1 into the algae mixture at a volume ratio of 1:1, and stir evenly to obtain the embedding solution; use ...

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Abstract

The invention relates to an immobilized algae ball and its preparation method and application. The preparation method of the immobilized algae ball is to first cultivate the microbial algae liquid, then prepare the algae mixed liquid and the algae cross-linking agent, add the microbial algae liquid to the algae mixed The embedding solution was obtained from the liquid, and the embedding solution was dripped into the algae cross-linking agent with a peristaltic pump to obtain immobilized algal bulbs. Acetyl tri-n-butyl citrate (ATBC) and chitosan are added to the algae mixture, which makes the obtained immobilized algae bulbs stronger and less prone to damage, and makes microalgae reproduce faster and more efficient in handling nitrogen and phosphorus. high. The immobilized algae balls prepared by the present invention only use acetyl tri-n-butyl citrate and chitosan as additives, and do not need to use a variety of materials to increase the mechanical strength of the immobilized algae balls, which simplifies the process and ensures the immobilized algae balls Good stability and activity, both of which are widely sourced, low in price, low in equipment requirements and low in toxicity to microorganisms, convenient for large-scale production, and cost-effective.

Description

technical field [0001] The invention relates to the technical field of aquaculture wastewater treatment, in particular to an immobilized algae ball and its preparation method and application. Background technique [0002] In recent years, my country's economic development is getting better and better, and people's living standards are gradually improving. In order to meet people's demand for seafood, the scale of my country's aquaculture industry is increasing every year, while seawater aquaculture has always used high-density and intensive farming methods. This kind of aquaculture The result of the method is that various pollutants in the water body, such as nitrogen and phosphorus, exceed the standard, and exceed the purification capacity of the water body itself, resulting in an imbalance in the breeding environment, which ultimately leads to a decrease in the survival rate of the culture and an increase in the cost of the culture. [0003] In order to solve the above-ment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/04C02F3/32
CPCC12N11/10C12N11/04C02F3/322
Inventor 胡春义
Owner 青岛鲜达物流科技有限公司
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