Strawberry vitamin C synthesis related gene FaGalLDH and application

A vitamin and gene technology is applied in the field of plant molecular genetics and genetic engineering to achieve the effect of improving salt tolerance, increasing vitamin C content and having broad application prospects.

Inactive Publication Date: 2020-12-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

L-galactose-1,4-lactone dehydrogenase (GalLDH) is a key enzyme in the synthesis of vitamin C, but there are few reports on the GalLDH...

Method used

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  • Strawberry vitamin C synthesis related gene FaGalLDH and application
  • Strawberry vitamin C synthesis related gene FaGalLDH and application
  • Strawberry vitamin C synthesis related gene FaGalLDH and application

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Embodiment Construction

[0030] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0031] 1. The target gene is cloned as follows:

[0032] design specific primers

[0033]

[0034] Using the cDNA of "Hongyan" strawberry fruit as a template, using FaGalLDH-F as an upstream primer, and FaGalLDH-R as a downstream primer, PCR reaction was performed to clone the FaGalLDH gene. The CDS region of FaGalLDH contains a complete open reading frame (ORF) , containing 1743bp, encoding 580 amino acids.

[0035] 2. Comparing the vitamin C content of "Hongyan" and "Zhangji" without FaGalLDH expression and "Hongyan" and "Zhangji" expressed by FaGalLDH.

[0036] The content of vitamin C can be determined by...

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Abstract

The invention discloses a strawberry vitamin C synthesis related gene FaGalLDH and application. The strawberry vitamin C synthesis related gene FaGalLDH has a nucleotide sequence shown as SEQ ID NO: 1. The invention further discloses application of the strawberry vitamin C synthesis related gene FaGalLDH in increasing the content of vitamin C and application of the strawberry vitamin C synthesis related gene FaGalLDH in improving the salt tolerance of plants. After the gene is over-expressed in arabidopsis thaliana, a transgenic plant with the content of vitamin C (AsA) remarkably increased isobtained, and the salt tolerance of the transgenic plant is improved. Experiments prove that the FaGalLDH gene can synthesize vitamin C in vitro, the optimal induction condition is found through optimization, the content of vitamin C in transgenic arabidopsis thaliana leaves can be remarkably increased through over-expression, and no obvious influence is caused to normal growth of plants. Proteinand an encoding gene thereof have important theoretical and practical significance in research of a plant vitamin C synthesis mechanism and improvement and stress resistance of the content of vitaminC of plants, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of plant molecular genetics and genetic engineering, in particular to a gene FaGalLDH related to the synthesis of strawberry vitamin C and its application. Background technique [0002] Strawberry (Fragaria×ananassa Duch.) is a perennial herb of the Rosaceae Fragaria genus. The fruit is bright in color, rich in aroma, sweet and sour, and has high nutritional and health value. It has the reputation of "Queen of Fruits". Strawberry fruit has been shown to have strong antioxidant activity associated with the vitamin C and polyphenolic compounds contained in the fruit. Vitamin C (also known as ascorbic acid) is a water-soluble vitamin, which not only has a positive preventive effect on human diseases such as scurvy, arteriosclerosis, and hypertension, but also plays an anti-oxidant, antioxidant role in the growth and development of plants. stimulating and other vital roles. In addition, vitamin C is also one o...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N15/82A01H5/00A01H6/20
CPCC12N9/001C12N15/8243C12N15/8273C12Y103/02003
Inventor 谢兴斌赵静方从兵薛浩孙培培冯欢顿宛宛
Owner ANHUI AGRICULTURAL UNIVERSITY
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