Compound lactobacillus leavening agent and application thereof
A technology of starter and compound milk, applied in the field of microorganisms, can solve problems such as blank and achieve good effect of probiotics
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preparation example Construction
[0040] The preparation method of the lactic acid bacteria powder involved in the following examples is as follows:
[0041] Dip the lactic acid bacteria solution in the bacteria preservation tube with an inoculation loop and streak it on the MRS solid medium, and culture it at 37°C for 36 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, and culture it at a constant temperature of 37°C for 12 hours. Obtain the seed liquid; inoculate the seed liquid into the MRS liquid culture medium according to the inoculation amount of 2% (v / v), cultivate at a constant temperature of 37° C. for 24 hours to obtain the culture liquid; centrifuge the culture liquid to collect the thalli; Concentration is 200g / L in the stachyose solution, obtains resuspension liquid; Freeze-drys resuspension liquid, obtains lactic acid bacteria bacterial powder;
[0042]Among them, the freeze-drying process: put the resuspension at -50°C for 4 hours; primary drying ...
Embodiment 1
[0049] Embodiment 1: the preparation of compound lactobacillus starter
[0050] Specific steps are as follows:
[0051] Option 1: Pulverize black tea leaves to 80 meshes to obtain pulverized tea leaves; mix the pulverized tea leaves with water, boil and extract for 30 minutes to obtain tea soup. During the extraction, the quality of pulverized tea leaves accounts for 20‰; centrifuge the tea soup at 3000g for 10min to obtain a supernatant; add 7% of the total mass of the supernatant to glucose, 5‰ of yeast protein, 2‰ of soybean peptone, and 5‰ of dipotassium hydrogen phosphate , 20‰ sodium citrate and 4% whey protein isolate to obtain a mixed solution; the mixed solution was first heated at 85°C for 15 minutes to sterilize, then concentrated in a vacuum at 45°C, and finally freeze-dried to obtain tea powder; Mix tea powder and Lactobacillus rhamnosus CCFM0528 bacteria powder in a sterile environment to obtain compound Lactobacillus starter 1. In Compound Lactobacillus starter...
Embodiment 2
[0057] Embodiment 2: the preparation of fermented tea beverage
[0058] Specific steps are as follows:
[0059] Option 1: Dissolve 3 g of the compound lactobacillus starter 1 obtained in Example 1 in 500 mL of pure water, and ferment at 37° C. for 12 hours to obtain fermented tea beverage 1 .
[0060] Solution 2: Take 3g of the compound lactobacillus starters 2-7 obtained in Example 1 and dissolve them in 500mL of pure water, and ferment at 37°C for 12 hours to obtain fermented tea beverages 2-7.
[0061] Solution 3: Take 3 g of the compound lactobacillus starter 8-12 obtained in Example 1 and dissolve them in 500 mL of pure water, and ferment at 37° C. for 12 hours to obtain fermented tea beverages 8-12.
[0062] Solution 4: Take 3 g of the compound lactobacillus starter 13-17 obtained in Example 1 and dissolve them in 500 mL of pure water, and ferment at 37° C. for 12 hours to obtain fermented tea beverages 13-17.
[0063] Solution 5: Take 3 g of the compound lactobacillus...
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Abstract
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Application Information
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