Banana ligoglucoside and preparation method and application thereof

A technology of oligodextran and dextran gel, which is applied in application, food preparation, pharmaceutical formulation, etc., can solve the problems of unclear mechanism of action, no identification of banana oligosaccharide extraction and separation structure, etc., so as to adjust the intestinal tract. Micro-ecological environment, easy promotion, low cost effect

Inactive Publication Date: 2012-06-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In recent years, there have been many reports on chitosan oligosaccharides, fructooligosaccharides, konjac glucomannan oligosaccharides, and oligosaccharides in some traditional Chinese medicine extracts, such as oligosaccharides extracted from rehmannia glutinosa, Panax notoginseng, and burdock, but there are no reports on banana oligosaccharides. The extraction and separation of bananas and the identification of related structures have been reported, but the clear material basis of bananas for treating constipation has not been reported, and the mechanism of action is still unclear.

Method used

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  • Banana ligoglucoside and preparation method and application thereof
  • Banana ligoglucoside and preparation method and application thereof
  • Banana ligoglucoside and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 prepares banana oligodextran according to the following method after the banana is peeled:

[0035] (1) Use an ordinary fruit and vegetable beater to beat the banana pulp, and the beating time is 3 minutes.

[0036] (2) The meat after water leaching and beating, the weight-to-volume ratio of feed to liquid is 1g: 3ml, the temperature is 70°C, the extraction time is 80min, and the extraction is 3 times; the 3 extractions are combined, and then precipitated with 4 times the volume of absolute ethanol 1. Centrifuge at a speed of 4000 rpm for 20 minutes, collect the precipitate, and obtain the banana crude oligosaccharide liquid;

[0037] (3) Sephadex G-100 was selected for chromatographic purification. The chromatographic column was 30cm long and 3cm in diameter. The gel chromatography conditions were: injection volume 10mL, flow rate 3s / drop, and collection within 5min to 7min eluate;

[0038] (4) Dry for 30 minutes under the conditions of vacuum degree 200...

Embodiment 2

[0040] After the banana is peeled, the banana oligodextran is prepared according to the following method:

[0041](1) Use an ordinary fruit and vegetable beater to beat the banana pulp, and the beating time is 2 minutes.

[0042] (2) The flesh after water leaching and beating, the weight-to-volume ratio of feed to liquid is 1g: 3ml, the temperature is 80°C, the extraction time is 90min, and the extraction is 3 times; the 3 extractions are combined, precipitated with 4 times the volume of absolute ethanol, Centrifuge at a speed of 5000 rpm for 15 minutes to obtain a crude banana oligosaccharide solution;

[0043] (3) SephadexG-100 was selected for chromatographic purification. The chromatographic column was 30cm long and 3cm in diameter. The gel chromatography conditions were: injection volume 10mL, flow rate 3s / drop, and collection within 8min to 10min eluate;

[0044] (4) Drying for 20 minutes under the conditions of vacuum degree 1500Pa and microwave power 2000W to obtain ...

Embodiment 3

[0046] After the banana is peeled, the banana oligodextran is prepared according to the following method:

[0047] (1) Use an ordinary fruit and vegetable beater to beat the banana pulp, the beating time is 2.5min,

[0048] (2) The meat after water leaching and beating, the weight-to-volume ratio of feed to liquid is 1g: 3ml, the temperature is 75°C, the extraction time is 80min, and the extraction is 3 times; the 3 extractions are combined, precipitated with 4 times the volume of absolute ethanol, Centrifuge at a speed of 4500 rpm for 16 minutes to obtain banana crude oligosaccharide liquid;

[0049] (3) Sephadex G-100 was selected for chromatographic purification. The chromatographic column was 30cm long and 3cm in diameter. The gel chromatography conditions were: injection volume 10mL, flow rate 3s / drop, and collection within 6min to 9min eluate;

[0050] (4) Dry for 25 minutes under the conditions of vacuum degree 1800Pa and microwave power 1600W to obtain banana oligode...

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Abstract

The invention discloses a banana ligoglucoside and a preparation method and the application thereof; the banana ligoglucoside has a structure shown by a formula (I); in the invention, the banana pulp is pulped, and then water extraction, ethanol sedimentation and vacuum microwave drying are carried out to obtain the banana ligoglucoside; the banana ligoglucoside is directly extracted from the banana and is natural oligosaccharide and has the characteristics of safety and no toxicity; compared with the stimulant laxatives, the banana ligoglucoside has effect of loosening bowel and relieving constipation, has good probiotic function on reducing pH value of intestinal canal, beneficial bacteria proliferation and inhibiting harmful bacteria, and is very suitable for the old to relieve the bowels difficultly owning to powerless transmission of the intestinal canal; in addition, the preparation method is simple, raw material and equipment are easy to obtain, the cost is low and the popularization is easy.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to a preparation method of functional food, in particular to a banana oligodextran, its preparation method and application. Background technique [0002] According to the Biochemical Nomenclature Nomenclature, oligosaccharides are defined as polymers containing 3 to 10 monosaccharides. Oligosaccharides are low-polymerized sugars with straight or branched chains formed by monosaccharides linked by glycosidic bonds. Studies in recent years have found that oligosaccharides are an important class of biological information molecules, which play an important role in the communication, recognition and interaction between cells. More and more recent studies have shown that oligosaccharides have various biological functions, such as: affecting the normal folding of polypeptide chains during protein biosynthesis; stabilizing the structure of proteins; improving the resistance of protei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A23L1/29A61K31/716A61P1/10A23L33/00
Inventor 王娟杨公明程燕锋
Owner SOUTH CHINA AGRI UNIV
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