Method for establishing mouse middle cerebral artery remote ischemia reperfusion model

A technology of ischemia-reperfusion and method establishment, which is applied in the field of establishment of ischemia-reperfusion model of distal middle cerebral artery in mice, and can solve the problems of blunt needle injury to brain tissue, long modeling time and high cost

Pending Publication Date: 2020-12-22
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The use of blunt needles in the above method has the risk of injuring brain tissue, and the cost is high, an

Method used

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  • Method for establishing mouse middle cerebral artery remote ischemia reperfusion model
  • Method for establishing mouse middle cerebral artery remote ischemia reperfusion model
  • Method for establishing mouse middle cerebral artery remote ischemia reperfusion model

Examples

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Embodiment 1

[0029] Example 1 Establishment of mouse middle cerebral artery distal ischemia-reperfusion model

[0030] (1) Weigh the male C57BL / 6 mice, induce anesthesia with 5% isoflurane in a closed bag, fix the mice on the operating table, and inhale 1.5-2% isoflurane through a face mask to maintain anaesthetization;

[0031] (2) The mice lie supine on the operating table, and the rectal temperature is monitored in real time during the operation, and the animal is kept warm at 36-37.5°C through a body temperature maintenance device;

[0032] (3) Use a razor to shave off the hair on the neck of the mouse, and disinfect it with anal iodine. Cut the skin in the middle of the neck, and bluntly separate the left and right subcutaneous tissues to expose the sternocleidomastoid muscle. Use a thin hook to pull apart the sternocleidomastoid muscle, and continue to separate it downward until you find the bilateral common carotid artery sheaths;

[0033] (4) Separate the bilateral common caroti...

Embodiment 2

[0039] Example 2 Establishment effect of mouse middle cerebral artery distal ischemia-reperfusion model

[0040] 18 male C57BL / 6 mice of about the same age and weight were randomly and equally divided into an experimental group and a sham operation group. To establish, the sham operation group was compared with the experimental group, except that the bilateral common carotid arteries and the distal vessels of the middle cerebral artery were not ligated, and other operations were consistent with the model. figure 2 Cerebral blood flow during the experiment.

[0041]Twenty-four hours after the above-mentioned modeling, the mice were anesthetized and sacrificed, and brain tissues were obtained. The brain tissue was stained with 2'3'5'-triphenyltetrazolium chloride (2,3,5-Triphenylte-trazoliumchloride, TTC). After TTC staining, the normal brain tissue was red; while the cerebral infarction tissue was unable to react due to the decrease of dehydrogenase activity, and the brain t...

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Abstract

The invention discloses a method for establishing a mouse middle cerebral artery remote ischemia reperfusion model, and belongs to the technical field of experimental animal models. According to the method, one line and a middle cerebral artery remote blood vessel are tied together in parallel for ligating to achieve blockage of the middle cerebral artery remote blood vessel, line releasing is conducted after blockage is conducted for 50 min, according to the method, the risk of damaging the blood vessel is reduced, blockage of the blood vessel can be well achieved, subsequent reperfusion is facilitated, according to the method, time for establishing a transient dMCAO model of a mouse is shortened, the modeling cost is reduced, the ischemia reperfusion model of dMCAO can be stably and effectively realized, repeatability is good, and a death rate is low.

Description

technical field [0001] The invention belongs to the technical field of experimental animal models, and in particular relates to a method for establishing a mouse middle cerebral artery distal ischemia-reperfusion model. Background technique [0002] Cerebrovascular disease is the leading cause of death in our country. Simulating the ischemia of human cerebrovascular disease through pathological models of small rodents (big and mice) is helpful to effectively evaluate the therapeutic effect of drugs in the preclinical stage. [0003] Currently, middle cerebral artery occlusion (MCAO) is the most common way to cause focal cerebral ischemia in small rodents. In the early days, some researchers used invasive methods such as transorbital or zygomatic arch removal to achieve proximal MCA occlusion, thereby constructing animal models, but this method of model construction is more damaging to animals. At present, less invasive methods are widely used, such as blocking the MCA blood...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/04
CPCA61D1/00A61D7/04
Inventor 刘向荣董雯赵顺英龚婷温少红
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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