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Inactivated vaccine for sheep rapid epidemic, sudden attack, lamb dysentery, enterotoxemia triple four-defense and preparation method thereof

A sheep rapid disease and enterotoxic blood technology, which is applied in the field of sudden attack, lamb dysentery, sheep rapid disease, enterotoxemia triple four-proof inactivated vaccine and its preparation, can solve the problem of low OD value of fermentation bacteria liquid and immune protection Poor yield, PH uncontrolled and other problems, to achieve the effect of high toxin production, improved effect, and reduced use and packaging costs

Active Publication Date: 2021-11-12
CHONGQING AULEON BIOLOGICALS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the limitation of the cultivation process conditions, the preparation method of the existing sheep rapid epidemic, sudden attack, lamb dysentery, enterotoxemia triple four-proof inactivated vaccine, the PH is not controlled, no feed is added, and the harvest time is only waiting for the harvest after inoculation. , so that under this process, the OD value of the fermentation broth is low, the number of viable bacteria is not high, and the amount of toxin produced is not high, and the immune protection rate is poor

Method used

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  • Inactivated vaccine for sheep rapid epidemic, sudden attack, lamb dysentery, enterotoxemia triple four-defense and preparation method thereof
  • Inactivated vaccine for sheep rapid epidemic, sudden attack, lamb dysentery, enterotoxemia triple four-defense and preparation method thereof
  • Inactivated vaccine for sheep rapid epidemic, sudden attack, lamb dysentery, enterotoxemia triple four-defense and preparation method thereof

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Embodiment 1

[0036] In this embodiment, the preparation method of the three-in-one four-prevention inactivated vaccine for sheep rapid epidemic, sudden attack, lamb dysentery, and enterotoxemia comprises the following steps:

[0037] 1) Primary seed preparation: each strain was inoculated in anaerobic liver broth medium, Clostridium putrefaciens C55-1 strain was cultured statically for 16 hours at 35°C, and Clostridium perfringens type B C58-1 strain and Clostridium perfringens D-type C60-2 strain were cultured statically for 16 hours at 37°C to obtain first-grade seeds;

[0038] Secondary seed preparation: Inoculate the first-grade seeds of each strain in the anaerobic broth medium, C. 1 strain and Clostridium perfringens D-type C60-2 strain were cultured statically for 16 hours at 37°C to obtain secondary seeds;

[0039]Bacterial culture: Prepare the culture medium for Clostridium putrefaction, each 100mL medium includes the following substances: 1.5g of beef extract powder, 1g of beef ...

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Abstract

The invention discloses a three-in-one four-prevention inactivated vaccine for sheep rapid disease, sudden attack, lamb dysentery and enterotoxemia and a preparation method thereof, belonging to the technical field of veterinary biological products. It includes an antigen and an adjuvant, and the antigen is composed of Clostridium putrefaciens C55‑1 strain, Clostridium perfringens type B C58‑1 strain and D type C60‑2 strain. The preparation method is as follows: inoculate the bacteria into the culture medium to obtain the first-grade seeds, and then inoculate the culture medium to obtain the second-grade seeds; inoculate the second-grade seeds into the culture medium to obtain the fermentation liquid; After the solution is mixed, inactivate and detoxify, add an adjuvant, shake fully and subpackage. The inactivated triple-combination four-defense vaccine of the present invention is through optimized culture conditions, the culture time is shortened, the bacterial density (OD value) is doubled, the toxicity of the semi-finished product is stronger, far greater than that of traditional craft products, and the immune protection effect of animals is greatly enhanced. The field of rapid epidemic, sudden attack, lamb dysentery, enterotoxemia triple four prevention inactivated vaccine has the value of popularization and application.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a triple-inactivated vaccine for sheep rapid disease, sudden attack, lamb dysentery and enterotoxemia and a preparation method thereof. Background technique [0002] Sheep plague mainly occurs in sheep and is an acute infectious disease caused by Clostridium putrefaciens. Sudden onset of sheep, the course of the disease is very short, characterized by hemorrhagic inflammatory damage to the abomasum mucosa, inflammation and necrosis of the digestive tract mucosa after the sheep is sick, and toxic shock, causing the sheep to die quickly, and the fatality rate is extremely high. [0003] Sheep quenching is caused by Clostridium perfringens type C, characterized by acute death, accompanied by peritonitis and ulcerative enteritis, 1-2-year-old sheep are prone to it. [0004] Lamb dysentery, also known as lamb shuttle dysentery, commonly known as red...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/08A61K39/39A61P31/04A61P1/12C12N1/20C12R1/145
CPCA61K39/08A61K39/39A61K2039/521A61K2039/54A61K2039/552A61K2039/70A61P1/12A61P31/04C12N1/20A61K2300/00
Inventor 王崇俊刘昕黎国华唐杰杨梅
Owner CHONGQING AULEON BIOLOGICALS
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