A gene polymorphism detection kit and detection method for guiding depression medication

Abstract

The invention discloses a gene polymorphism detection kit and detection method for guiding depression medication, the kit includes amplification primers and extension primers for detecting 55 gene polymorphism sites for guiding depression medication, PCR reaction reagents, PCR product purification reagents, single base extension reaction reagents, negative quality control products, positive quality control products; the 5' end of the amplification primer includes a protective base sequence, and the 5' end of the extension primer includes base sequence; the detection method includes steps: (1) multiplex PCR, amplifying the DNA region where the polymorphism site is located; (2) purifying the PCR product; (3) performing multiplex PCR on the purified PCR product Single-base extension to obtain extension products; (4) Purify the extension products; (5) Mass spectrometer detection; (6) Interpretation of results; the present invention can comprehensively detect multiple genetic polymorphisms that guide depression medication at one time point.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a detection kit and a detection method for a gene polymorphism site used for guiding depression medication. Background technique [0002] As of 2015, more than 300 million people around the world suffer from depression, accounting for about 4.4% of the global population, and more than 800,000 people with depression commit suicide every year. Among them, the number of depression patients in China exceeds 50 million, ranking first in the world. [0003] Depression patients often face a long cycle of drug selection (generally it takes about one year to determine the appropriate drug, which may delay the best time for treatment), poor treatment effect or risk of drug side effects (the effective rate of the drug is about 30% ~50%), greatly increasing the cost of treatment, but also caused a great burden to the country and society. [0004] Pharmacogenomics detection helps doctors find indiv...

AI Technical Summary

Problems solved by technology

[0005] At present, the mainstream of gene polymorphism detection on the market is high-throughput sequencing (NGS), but its sequencing costs are high and time-consuming, so there are certain limitations in clinical application; real-time fluorescent quantitative PCR can also be used for multi-well sequencing. Reaction detection, although it meets the timeliness problem, its throughput is low, and its practical applicability is not strong when more than 10 sites need to be detected

[0006] The existing technical problems are: common detection techniques, such as sequencing, fluorescent quantitative PCR, etc., all need to detect the sites one by one, when there are many sites, the operation is complicated and the cost is high; while the existing nucleic acid based on multiplex PCR Mass spectrometry detection products of the same kind are single, and the selection and combination of gene loci are relatively few compared with traditional sequencing, real-time fluorescent quantitative PCR and other methods of detection products, lacking the ability to simultaneously and comprehensively detect multiple genes that guide depression medication Methods and Products of Polymorphism

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