Abnormal sugar chain glycoprotein detection reagent and preparation method thereof

Abstract

The invention relates to an abnormal sugar chain glycoprotein detection reagent and a preparation method thereof. The detection reagent takes purified water as a solvent, and comprises the following components: 10-50mg / L of mixed lectin, 1-3g / L of disperse blue and 4-20mg / L of a divalent metal ion stabilizer, wherein the pH of the detection reagent is adjusted to be greater than or equal to 9.0 through a pH regulator; the mixed lectin is a mixture of concanavalin, datura agglutinin, lentil agglutinin, wheat germ agglutinin, E-type red kidney bean agglutinin, L-type red kidney bean agglutinin,citrus fruit powder spore agglutinin, peanut agglutinin, castor agglutinin I and Korean locust agglutinin; and the mixed lectin is subjected to PEG pre-modification treatment. The design principle ofthe abnormal sugar chain glycoprotein detection reagent is that abnormal sugar chain glycoprotein in blood is condensed into particles through the mixed lectin, wherein the disperse blue participatesin condensation reaction and serves as a color marker, and observation and identification of the condensate particles are facilitated.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and in particular relates to an abnormal sugar chain glycoprotein detection reagent and a preparation method thereof. Background technique [0002] As an important post-translational modification, protein glycosylation plays an important role in protein structure and function. It has been found that in mammalian cells, about 50% of proteins are glycosylated. It plays a key role in many cellular functions such as adhesion, cell-to-cell interactions, and growth and development. [0003] Abnormal glycan glycoproteins are mainly caused by incomplete glycosylation or new glycosyltransferases are activated to generate new glycosylation. A large number of studies have shown that the production of abnormal glycan glycoproteins is closely related to tumors. Fetal protein, transferrin, alkaline phosphatase, r-glutamyl transferase, human chorionic gonadotropin, T antigen, a1 antitrypsin, prostatic...

AI Technical Summary

Problems solved by technology

[0007] Second, the compatibility of lectins is unreasonable, and cannot effectively take into account broad-spectrum and accuracy;

[0008] Third, the composite lectin has not been pretreated effectively, and its stability is insufficient, which affects the reliability of reagent detection;