Abnormal sugar chain glycoprotein detection reagent and preparation method thereof

A technology for detecting reagents and glycoproteins, which is applied in the field of immunoassays, can solve problems such as the inability to effectively balance broad-spectrum and accuracy, the lack of effective pretreatment of compound lectins, and the unreasonable compatibility of lectins, etc., to achieve both broad-spectrum Performance and accuracy, improved stability, and guaranteed reliability

Pending Publication Date: 2020-12-25
浙江瑞生医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Second, the compatibility of lectins is unreasonable, and cannot effectively take into account broad-spectrum and accuracy;
[0008] Third,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Abnormal glycan glycoprotein detection reagent, the detection reagent uses purified water as a solvent, and the content of each component in the detection reagent is as follows: mixed lectin 10mg / L, disperse blue 1g / L, divalent metal ion stabilizer 4mg / L , the detection reagent is adjusted to pH 9.0 by a pH regulator;

[0054] The mixed lectin is concanavalinum lectin, Datura lectin, lentil lectin, wheat germ lectin, E-type red kidney bean lectin, L-type red kidney bean lectin, citrus fruit powder lectin, peanut A mixture of lectins, Ricinus communis lectin I and Sophora japonica lectins pre-treated with PEG.

[0055] Among the mixed lectins, the mass ratio of concanavalina lectin, Datura lectin and lentil lectin is 1:1:1; wheat germ lectin, E-type red kidney bean lectin and L-type red kidney bean lectin The mass ratio of lectin was 4:1:1, and the mass ratio of peanut lectin, castor lectin I and Korean sophora lectin was 2:1:1.

[0056] The steps of PEG pre-modification...

Embodiment 2

[0065] Abnormal glycan glycoprotein detection reagent, the detection reagent uses purified water as a solvent, and the content of each component in the detection reagent is as follows: mixed lectin 50mg / L, disperse blue 3g / L, divalent metal ion stabilizer 20mg / L , the detection reagent is adjusted to pH 10.0 by a pH regulator;

[0066] The mixed lectin is concanavalinum lectin, Datura lectin, lentil lectin, wheat germ lectin, E-type red kidney bean lectin, L-type red kidney bean lectin, citrus fruit powder lectin, peanut A mixture of lectins, Ricinus communis lectin I and Sophora japonica lectins pre-treated with PEG.

[0067] Among the mixed lectins, the mass ratio of concanavalina lectin, Datura lectin and lentil lectin is 1:1:1; wheat germ lectin, E-type red kidney bean lectin and L-type red kidney bean lectin The mass ratio of lectin was 4:1:1, and the mass ratio of peanut lectin, castor lectin I and Korean sophora lectin was 2:1:1.

[0068] The steps of PEG pre-modifica...

Embodiment 3

[0077] Abnormal glycan glycoprotein detection reagent, the detection reagent uses purified water as a solvent, and the content of each component in the detection reagent is as follows: mixed lectin 30mg / L, disperse blue 2g / L, divalent metal ion stabilizer 10mg / L , the detection reagent is adjusted to pH 11.0 by a pH regulator;

[0078] The mixed lectin is concanavalinum lectin, Datura lectin, lentil lectin, wheat germ lectin, E-type red kidney bean lectin, L-type red kidney bean lectin, citrus fruit powder lectin, peanut A mixture of lectins, Ricinus communis lectin I and Sophora japonica lectins pre-treated with PEG.

[0079] Among the mixed lectins, the mass ratio of concanavalina lectin, Datura lectin and lentil lectin is 1:1:1; wheat germ lectin, E-type red kidney bean lectin and L-type red kidney bean lectin The mass ratio of lectin was 4:1:1, and the mass ratio of peanut lectin, castor lectin I and Korean sophora lectin was 2:1:1.

[0080] The steps of PEG pre-modifica...

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PUM

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Abstract

The invention relates to an abnormal sugar chain glycoprotein detection reagent and a preparation method thereof. The detection reagent takes purified water as a solvent, and comprises the following components: 10-50mg/L of mixed lectin, 1-3g/L of disperse blue and 4-20mg/L of a divalent metal ion stabilizer, wherein the pH of the detection reagent is adjusted to be greater than or equal to 9.0 through a pH regulator; the mixed lectin is a mixture of concanavalin, datura agglutinin, lentil agglutinin, wheat germ agglutinin, E-type red kidney bean agglutinin, L-type red kidney bean agglutinin,citrus fruit powder spore agglutinin, peanut agglutinin, castor agglutinin I and Korean locust agglutinin; and the mixed lectin is subjected to PEG pre-modification treatment. The design principle ofthe abnormal sugar chain glycoprotein detection reagent is that abnormal sugar chain glycoprotein in blood is condensed into particles through the mixed lectin, wherein the disperse blue participatesin condensation reaction and serves as a color marker, and observation and identification of the condensate particles are facilitated.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and in particular relates to an abnormal sugar chain glycoprotein detection reagent and a preparation method thereof. Background technique [0002] As an important post-translational modification, protein glycosylation plays an important role in protein structure and function. It has been found that in mammalian cells, about 50% of proteins are glycosylated. It plays a key role in many cellular functions such as adhesion, cell-to-cell interactions, and growth and development. [0003] Abnormal glycan glycoproteins are mainly caused by incomplete glycosylation or new glycosyltransferases are activated to generate new glycosylation. A large number of studies have shown that the production of abnormal glycan glycoproteins is closely related to tumors. Fetal protein, transferrin, alkaline phosphatase, r-glutamyl transferase, human chorionic gonadotropin, T antigen, a1 antitrypsin, prostatic...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803G01N33/68
Inventor 陆修委郭清
Owner 浙江瑞生医疗科技有限公司
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