Application of dendrobium officinale polysaccharide in preparation of drug for treating tumors by targeting tumor-associated macrophages

A technology related to Dendrobium officinale polysaccharide and tumor, which is applied in the directions of antitumor drugs, drug combinations, and pharmaceutical formulations to achieve the effects of promoting tumor killing activity, inhibiting immune escape, and inhibiting tumor growth.

Inactive Publication Date: 2020-12-29
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the treatment of tumors by the homogeneous components of Dendrobium officinale polysaccharides targeting TAMs. Obviously, the polysaccharides of Dendrobium officinale targeting TAMs will provide a new strategy for tumor treatment

Method used

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  • Application of dendrobium officinale polysaccharide in preparation of drug for treating tumors by targeting tumor-associated macrophages
  • Application of dendrobium officinale polysaccharide in preparation of drug for treating tumors by targeting tumor-associated macrophages
  • Application of dendrobium officinale polysaccharide in preparation of drug for treating tumors by targeting tumor-associated macrophages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of dendrobium officinale polysaccharide

[0029] Dendrobium officinale Kimura et Migo was collected from Huoshan, and its fresh strips were dried at 70°C to constant weight and crushed for later use. Accurately weigh 100g of dried Dendrobium officinale powder, add 80% ethanol according to the ratio of material to liquid 1:60, stir and extract at room temperature for 24 hours, centrifuge at 5000g for 10 minutes, collect the precipitate, dry the precipitate to remove ethanol, press 1: Add distilled water at a liquid ratio of 60, stir and extract at 80°C for 2 hours, collect the supernatant by gauze filtration, repeat the water extraction of the residue twice, and finally combine the filtrate three times, and concentrate the extracted filtrate under reduced pressure (65°C), and precipitate with 80% ethanol , deproteinized by Sevag method, dialyzed (3500Da, 24h), and freeze-dried to obtain the crude polysaccharide of Dendrobium officinale. The...

Embodiment 2

[0033] Example 2: DOP anti-tumor activity evaluation

[0034] After the mice were adaptively fed for one week, 150 male C57BL / 6J mice were selected. Except for the normal group (Normal, 10-15 mice), the rest of the mice were inoculated with Hepa 1-6 liver cancer cells, and the growing Hepa1-6 cell suspension in logarithmic phase (2×10 6 each / mL), inoculated in the right armpit of 100 C57BL / 6J mice after disinfection, and each mouse was inoculated with 0.2mL (2×10 6 cells / mL), and press the injection point to prevent cell leakage. During the modeling period, the tumor formation in mice was closely observed. After 3-7 days, when the tumor grew to the touch (the tumor grew to about 0.5cm×0.5cm), use The long diameter (a) and short diameter (b) of the tumor were measured with a vernier caliper, and those with too large or small tumor mass were excluded. Finally, the experiment finally divided the tumor-bearing mice that met the criteria into 5 groups randomly: the model group (Mo...

Embodiment 3

[0038] Example 3: DOP anti-tumor immunomodulatory effect

[0039] After the tumor tissues of each group were taken out and weighed, they were respectively placed in sterile plates containing PBS (FBS, 0.01M, pH7.4) buffer solution containing an appropriate amount of 5% fetal bovine serum, and the tumor tissues were cut with ophthalmic surgical scissors on ice. Minced (1mm), add 2mL of digestion solution containing type IV collagenase (1.5mg / mL, 500U / mg) and DNase-I (0.5mg / mL, 3000U / mg) to every 100mg of tumor tissue, at 37°C, 120rpm After digestion for 60 min under the condition of / mim, transfer to ice and add PBS buffer solution containing 20% ​​fetal bovine serum to stop the digestion, filter through 200 mesh gauze, collect the cell filtrate, centrifuge at 400g for 5 min at 4°C, discard the supernatant to collect the precipitate, After resuspending the pellet in FBS buffer, add red blood cell lysate at a ratio of 1:3, lyse on ice for 15 minutes, centrifuge at 400g for 5 min...

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Abstract

The invention relates to an application of dendrobium officinale polysaccharide in preparation of a drug for treating tumors by targeting tumor-associated macrophages, and belongs to the technical field of botanical drugs. Through the combination of in-vitro and in-vivo experiments, it is found for the first time that the dendrobium officinale polysaccharide can recognize TLR2 receptors on the surfaces of tumor-associated macrophage membranes in a targeted manner, the TAMs are induced to be polarized from M2 type to M1 type through mediation of the TLR2 receptors, then the tumor killing activity of CD8+T cells is promoted, immune escape generated by Tregs is inhibited, and therefore an anti-tumor effect is achieved synergistically. The application of the dendrobium officinale polysaccharide as the anti-tumor drug is developed, and a new possibility is provided for treating tumors by targeting the TAMs.

Description

technical field [0001] The invention belongs to the technical field of plant medicines, and specifically relates to the application of dendrobium officinale polysaccharides in the preparation of medicines targeting and recognizing tumor-associated macrophages for treating tumors. Background technique [0002] Tumor is a new organism formed by the body under the action of various tumorigenic factors, the cells of local tissues lose their growth regulation at the gene level, resulting in clonal abnormal proliferation, and often manifests as a local mass. In recent years, the morbidity and mortality of tumors have gradually increased, and it has become one of the main threats to human health. According to the 2019 global cancer statistics report, there are 18.1 million new cases and 9.6 million deaths every year in the world. Among them, my country is a country with a high incidence of cancer. At present, my country's annual new cases account for about 21% of the world's total,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00
CPCA61K31/715A61P35/00
Inventor 罗建平王红燕李强明査学强潘利华
Owner HEFEI UNIV OF TECH
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