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Method for evaluating moisturizing effect of cosmetics

A technology for cosmetics and water replenishment, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of long detection time, high professionalism, and low accuracy, and achieve short experiment cycle, strong comparability, and low cost. low effect

Inactive Publication Date: 2020-12-29
南京新环检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of long detection time, high cost, low accuracy and high professionalism of the traditional method for evaluating the moisturizing effect of cosmetics, the present invention provides a method for evaluating cosmetics with high efficiency, low cost, high accuracy and easy-to-understand experimental results. Methods of moisturizing effect

Method used

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  • Method for evaluating moisturizing effect of cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Randomly select 120 melanin allele mutant translucent AL strain zebrafish 2 days after fertilization (2dpf) in a six-well plate, each well has a capacity of 3 mL, and each well is treated with 30 zebrafish, and 3.125 μL of / mL Cosmetic 1# to be tested and 9mg / mL0.9wt% sodium chloride solution, treated zebrafish for 24 hours in a water-soluble exposure mode, and established a sample group;

[0028] (2) Select zebrafish 2 days after fertilization with the same number as in step (1), without any treatment, and establish a blank control group;

[0029] (3) Select zebrafish 2 days after fertilization with the same number as in step (1), add 9mg / mL 0.9wt% sodium chloride solution, and treat zebrafish with water-soluble exposure for 24h to establish a microosmotic water-deficient model group;

[0030] (4) Add 3 mL of culture water to the experimental group, blank control group and inducer control group, respectively, and incubate at a constant temperature of 28° C. for 18 ...

Embodiment 2

[0034](1) Randomly select 120 melanin allele mutant translucent AL strain zebrafish 2 days after fertilization (2dpf) in a six-well plate, each well has a capacity of 3 mL, and each well is treated with 30 zebrafish, and 6.25 μL of / mL Cosmetic 2# to be tested and 9mg / mL0.9wt% sodium chloride solution, treated zebrafish for 24h in water-soluble exposure mode, and established a sample group;

[0035] (2) Select zebrafish 2 days after fertilization with the same number as in step (1), without any treatment, and establish a blank control group;

[0036] (3) Select zebrafish 2 days after fertilization with the same number as in step (1), add 9mg / mL 0.9wt% sodium chloride solution, and treat zebrafish with water-soluble exposure for 24h to establish a microosmotic water-deficient model group;

[0037] (4) The experimental group, the blank control group and the inducer control group were respectively added with 3 mL of breeding water, and cultured at a constant temperature at 28° C....

Embodiment 3

[0040] (1) Randomly select 120 translucent AL strain zebrafish with melanin allele mutation 2 days after fertilization (2dpf) in a six-well plate, each well has a capacity of 3 mL, and each well is treated with 30 zebrafish, adding 12.5 μL / mL Cosmetic 3# to be tested and 9mg / mL0.9wt% sodium chloride solution, treated zebrafish for 24 hours in a water-soluble exposure mode, and established a sample group;

[0041] (2) Select zebrafish 2 days after fertilization with the same number as in step (1), without any treatment, and establish a blank control group;

[0042] (3) Select zebrafish 2 days after fertilization with the same number as in step (1), add 9mg / mL 0.9wt% sodium chloride solution, and treat zebrafish with water-soluble exposure for 24h to establish a microosmotic water-deficient model group;

[0043] (4) The experimental group, the blank control group and the inducer control group were respectively added with 3 mL of breeding water, and incubated at the same tempera...

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Abstract

The invention relates to the technical field of cosmetic effect evaluation, and particularly relates to a method for evaluating the moisturizing effect of cosmetics. The method comprises the followingsteps: (1) establishing a sample group; (2) establishing a blank control group; (3) establishing a micro-seepage water shortage model group; (4) performing constant-temperature culture; and (5) taking a picture under an anatomical microscope, storing the picture, calculating the area of the tail of each group of zebra fish, and calculating the moisturizing effect of the cosmetics to be tested according to the following formula: wherein A is the average area of the tail of each group of zebra fish. According to the method, the effect of the cosmetics is evaluated by using zebra fish model organisms, the experimental period is short, the cost is low, embryos and juvenile fishes are transparent, the change of various tissues and organs can be directly observed under the microscope, and the experimental result is intuitive and easy to understand.

Description

technical field [0001] The invention relates to the technical field of cosmetic efficacy evaluation, in particular to a method for evaluating the moisturizing efficacy of cosmetics. Background technique [0002] Moisturizing is the eternal theme of cosmetic care. When the air temperature drops, the stratum corneum of the skin cannot adjust enough moisturizing factors in time, the activity of the oil glands decreases, and the oil and moisture on the face decrease. By using skin care products with corresponding effects, the skin can be moisturized and moisturized. The process of preventing skin evaporation, improving microcirculation, and enhancing skin moisture is called moisturizing. There are many kinds of moisturizers in cosmetics. Commonly used moisturizers mainly include small molecular polyols, such as glycerin and propylene glycol, macromolecular compounds, such as hyaluronic acid, polyglutamic acid, etc., as well as lactic acid and sodium lactate, pyrrolidone carboxyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 戴明珠郭杰朱晓宇徐懿乔郭胜亚黄燕烽张勇曹兵兵李春启
Owner 南京新环检测科技有限公司