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UPC2-PDA-Q-Tof/MS detection method for 42 effective components in ganoderma lucidum wine

A technology of active ingredients and detection methods, applied in the field of functional wine, can solve the problems of low efficiency, great difference in the extraction rate of active ingredients, time-consuming, etc., and achieve the effect of simple pre-treatment

Active Publication Date: 2020-12-29
ANHUI RUISIWEIER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current production process of Ganoderma lucidum wine, various and varied techniques have appeared, including direct soaking, boiling wine, crushing and soaking, high and low soaking, and vacuum extraction. Yes, there is also fermentation by adding bacteria, but the extraction rate of active ingredients in various extraction methods is very different, and there are many types of active ingredients in Ganoderma lucidum, and the dissolution of various substances is not the same, which also hinders the development of Ganoderma lucidum wine
[0004] At present, the existing data show that the detection methods of active ingredients of Ganoderma lucidum are mainly high-performance liquid chromatography (HPLC), mass spectrometry, thin-layer chromatography, etc., the detection flux is small, and the content of active ingredients of Ganoderma lucidum cannot be reflected as a whole, and the efficiency is low. time consuming

Method used

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  • UPC2-PDA-Q-Tof/MS detection method for 42 effective components in ganoderma lucidum wine
  • UPC2-PDA-Q-Tof/MS detection method for 42 effective components in ganoderma lucidum wine
  • UPC2-PDA-Q-Tof/MS detection method for 42 effective components in ganoderma lucidum wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Soak wine sample assay

[0053] Pre-treatment of soaked wine samples: Soak 500g of ganoderma lucidum purchased in the market in 5000mL of white wine and stir once a day. After 30 days, take 50mL of ganoderma lucidum wine and freeze-dry it in a 50mL centrifuge tube, add 2mL of formic acid-water-methanol mixed solution, mix well Pass through a 0.22μm filter membrane to be tested;

[0054] For the determination of 42 active ingredients in the soaked wine sample, the above-mentioned samples to be tested were injected according to the liquid phase conditions and mass spectrometry conditions in 1.4, and the results were as follows:

[0055] After testing, 500g of Ganoderma lucidum was directly soaked in 5000mL of liquor for 30 days, and it was detected that β-sitosterol 96.5μg / L, Ganoderma aldehyde A 416.12μg / L, Ganoderma acid S 693.45μg / L, Ganoderma alcohol A 715.46μg / L, Ganoderma lucidum Acid Y 882.36μg / L, Danzhil B 249.57μg / L, Ganoderma acid X346.32μg / L, Gan...

Embodiment 2

[0056] Embodiment 2: extract solution assay

[0057] Preparation of Ganoderma lucidum extract: Soak 4000g of Ganoderma lucidum purchased in the market in 45kg of 60% (V / V) alcohol aqueous solution overnight, heat at 85°C under reflux for 4h, heat and concentrate at 80°C for 3h to obtain 3.5kg of extract,

[0058] Pretreatment of Ganoderma lucidum extract: take 20mL of Ganoderma lucidum extract, filter it with 8 layers of gauze, centrifuge at 8000r / min for 5 minutes, then take 10mL of supernatant and freeze-dry it in a centrifuge tube, add 50mL of formic acid-water-methanol mixed solution, mix well After passing through a 0.22μm filter membrane to be tested.

[0059] For the determination of 42 active ingredients in Ganoderma lucidum extract, the above-mentioned samples to be tested were injected according to the liquid phase conditions and mass spectrometry conditions in 1.4, and the results were as follows:

[0060] After testing, the Ganoderma lucidum extract contains β-sit...

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Abstract

The invention discloses a UPC2-PDA-Q-Tof / MS detection method for 42 effective components in ganoderma lucidum wine. The method comprises the steps of preparing a standard substance, pretreating a sample, establishing chromatographic conditions, mass spectrum conditions and a qualitative and quantitative method and the like to quickly determine the 42 effective components in the ganoderma lucidum wine. The method is simple in pretreatment, the linear correlation coefficient is greater than 0.99, the recovery rate is 85% -115%, and the relative standard deviation is within 10%, so that the method can be applied to qualitative and quantitative analysis of the effective components in the ganoderma lucidum wine and can provide important reference significance for quality control of the ganoderma lucidum wine.

Description

technical field [0001] The invention belongs to the field of functional wine, and in particular relates to a UPC2-PDA-Q-Tof / MS detection method for 42 active ingredients in Ganoderma lucidum wine. Background technique [0002] my country has a long history of brewing wine, with a wide variety of varieties, mostly white wine, fruit wine and rice wine, but these wines generally have high alcohol content, and excessive drinking will cause harm to the body. As people pay more attention to their own health, various low-alcohol medicinal wines emerge at the historic moment, and Ganoderma lucidum wine is one of them. Ganoderma lucidum is the dry fruiting body of the polyporaceae fungus Chizhi or Zizhi. Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine, known as the "fairy grass". Ancient and modern pharmacological and clinical studies have proved that Ganoderma lucidum does have the effect of preventing and curing diseases, and prolonging life....

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N30/8675G01N2030/045
Inventor 马金同曹润洁何宏魁李安军汤有宏刘国英丁峰刘智慧芮君君孟涛周鹏磊
Owner ANHUI RUISIWEIER TECH