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Preparation method, printing method and application of 4D printing intelligent hydrogel

A hydrogel, intelligent technology, applied in biochemical equipment and methods, applications, household appliances, etc., can solve the problems of slow response to external stimuli, poor biocompatibility, intricate rheological characterization, etc., and achieve good printability. , good adhesion, good 4D deformation properties

Active Publication Date: 2021-01-08
大连芯鸿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of general bio-inks such as slow response to external stimuli, intricate rheological characterization, and poor biocompatibility, it is very important to prepare bio-inks with good printability, responsiveness to external stimuli, and biocompatibility. of

Method used

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  • Preparation method, printing method and application of 4D printing intelligent hydrogel
  • Preparation method, printing method and application of 4D printing intelligent hydrogel
  • Preparation method, printing method and application of 4D printing intelligent hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of 4D printed smart hydrogel

[0032] Add 40 g of cellulose nanofibers (NFC), 2 g of N,N-dimethylacrylamide, 0.1 g of photoinitiator Irgacure 2959, 0.1 g of glucose oxidase, 1 g of glucose, and 4g of endothelial cell growth factor (ECGF) at 200μg / ml and 2g of heparin at a concentration of 20μg / ml were uniformly mixed and reacted, placed in an open mechanical stirring device, and nitrogen was introduced for protection; Slowly add 2g of magnesium lithium silicate, use 10g of deionized water to rinse the feeding caliber of the three-necked flask, and flush the material attached to the mouth of the three-necked flask into the three-necked flask; take out the three-necked flask, and react at room temperature for 0.5h to obtain the target material 4D printing smart hydrogels.

Embodiment 2

[0033] Example 2 Preparation of 4D printed smart hydrogel

[0034] Add 50 g of cellulose nanofibers (NFC), 3 g of N,N-dimethylacrylamide, 0.1 g of photoinitiator Irgacure 2959, 0.1 g of glucose oxidase, 1.5 g of glucose, and Concentration of 4g of endothelial cell growth factor (ECGF) of 200μg / ml and concentration of 20μg / ml of 2g of heparin, mixed evenly and reacted, placed in an open mechanical stirring device, fed with nitrogen for protection; Slowly add 2.5g lithium magnesium silicate once, use 10g deionized water to rinse the feeding aperture of the three-necked flask, and flush the material attached to the mouth of the three-necked flask into the three-necked flask; take out the three-necked flask, and react at room temperature for 0.5h to obtain Target material 4D printing smart hydrogel.

Embodiment 3

[0035] Example 3 Preparation of 4D printed smart hydrogel

[0036] 50 g of cellulose nanofibers (NFC), 4 g of N,N-dimethylacrylamide, 0.1 g of photoinitiator Irgacure 2959, 0.1 g of glucose oxidase, 1.5 g of glucose, and 200 μg / ml 4g of endothelial cell growth factor (ECGF) and 2g of heparin with a concentration of 20μg / ml were uniformly mixed and reacted, placed in an open mechanical stirring device, and nitrogen was passed into the protection; then 4g of silicic acid was slowly added to the three-necked flask three times For magnesium and lithium, use 10g of deionized water to rinse the feeding caliber of the three-necked flask, and pour the substance attached to the mouth of the three-necked flask into the three-necked flask; take out the three-necked flask, and react at room temperature for 0.5h to obtain the target material 4D printing intelligent hydrogel glue.

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Abstract

The invention belongs to the technical field of biological ink, and relates to a preparation method, a printing method and an application of 4D printing intelligent hydrogel. The preparation method comprises the following steps: (1) sequentially adding cellulose nanofibers, N,N-dimethyl acrylamide, a photoinitiator, glucose oxidase, glucose, endothelial cell growth factors and heparin into a three-necked flask, after mixing, putting the mixture into a mechanical stirring device, and introducing nitrogen for protection; (2) slowly adding magnesium lithium silicate into the three-necked flask multiple times; (3) flushing substances attached to the flask opening into the three-necked flask by adopting deionized water; and (4) taking out the three-necked flask, and reacting at room temperatureto obtain the target material. The 4D printing intelligent hydrogel prepared by the invention has good printability and 4D deformation performance, and has corresponding properties under external stimulation. After the hydrogel is coated, endothelial cells can show good adhesion in a cell culture experiment.

Description

technical field [0001] The present invention relates to a preparation, printing method and application of a 4D printing intelligent hydrogel, and belongs to the technical field of biological inks. Background technique [0002] 3D bioprinting technology is a technology that has emerged in recent years, and it has been tried to build complex tissues and organs. 4D printing uses the same technology as 3D printing, whereby a computer is programmed to deposit material in successive layers to create three-dimensional objects. However, 4D printing adds a dimension of temporal transformation. This is thus a programmable substance which, after the manufacturing process, the printed product reacts to the parameters of the environment (humidity, temperature, etc.) and changes its original form accordingly over time. [0003] Scaffold materials are one of the three elements of tissue engineering research, which require not only bio-3D printability, but also considerable mechanical str...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L51/02C08K3/34C08F251/02C08F220/54C09D11/107C09D11/03C12N5/071B33Y70/10
CPCC08J3/075C08F251/02C09D11/107C09D11/03C12N5/069B33Y70/10C12N2535/10C12N2533/30C12N2501/71C12N2501/10C12N2501/91C08J2351/02C08K3/34C08F220/54
Inventor 高志刚罗勇柳国玉
Owner 大连芯鸿生物科技有限公司
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