Method, system and device for fast imaging of biological ultrathin slices carried by plastic strips
A technology of ultra-thin sectioning and imaging method, which can be used in measurement devices, analysis materials, material analysis using wave/particle radiation, etc., and can solve the problems of poor imaging contrast, slow speed, and electron irradiation damage.
Active Publication Date: 2021-03-16
INST OF AUTOMATION CHINESE ACAD OF SCI
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Problems solved by technology
[0004] In order to solve the above-mentioned problems in the prior art, that is, in order to solve the problems of slow speed, poor imaging contrast and susceptibility to electron radiation damage in the imaging process of biological ultrathin slices on the surface of plastic strips in the prior art
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[0031] In order to make the embodiments, technical solutions and advantages of the present invention more obvious, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Example. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of the present invention, and are not intended to limit the protection scope of the present invention.
[0032] A rapid imaging method for biological ultrathin slices carried by plastic strips of the present invention comprises the following steps.
[0033] Step S100, obtaining biological ultrathin slices, collecting them on plastic strips, fixing the plastic strips to the surface of the wafer, and performing carbon plating on the surface of the biological ultrathin slices.
[0034] Step S200, ...
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Abstract
The invention belongs to the technical field of surface structure characterization of biological materials, and in particular relates to a rapid imaging method, system and device for biological ultrathin slices carried by plastic strips, aiming at solving the problem of the imaging process of biological ultrathin slices on the surface of plastic strips in the prior art The problems of slow speed, poor imaging contrast and susceptibility to electron radiation damage. This application prepares and takes care of the ultra-thin slices of biological slices carried by plastic strips before electron microscope imaging; avoids electron radiation damage on the surface of imaging biological slice materials under high beam current density of the electron microscope, and realizes rapid acquisition of electron microscope images. This method obtains a relatively high imaging beam current density by adjusting the aperture of the objective lens aperture of the electron microscope, controls the injection speed and action time of specific metered electrons on the sample surface, and forms electrons between the biological ultrathin slice and the carrying plastic strip. Permanent protective layer, thereby avoiding electron irradiation damage on the surface of biological slices under high beam current density during official imaging, and improving the imaging speed of the electron microscope.
Description
technical field [0001] The invention belongs to the technical field of surface structure characterization of biological materials, in particular to a method, system and device for rapid imaging of biological ultrathin slices carried by plastic strips. Background technique [0002] Scanning electron microscopy (SEM) imaging is the mainstream technology in 3D reconstruction. When it is used to obtain the fine microstructure of the surface of biological ultrathin slices, its imaging signals include secondary electrons and backscattered electrons. The source of the secondary electron signal is more than ten nanometers deep on the surface of the sample, which is more sensitive to surface information. Some micron-sized pollutants are obvious under secondary electron imaging. Insensitive to pollution, the imaging effect is delicate and the picture quality is clear. For biological ultrathin slices, its material composition is the polymer product of epoxy resin and acid anhydride cr...
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IPC IPC(8): G01N23/2251G01N23/2202
CPCG01N23/2202G01N23/2251
Inventor 张丽娜马宏图李琳琳李国庆韩华
Owner INST OF AUTOMATION CHINESE ACAD OF SCI