Improved flow cytometer based on optical fiber integrated micro-fluidic chip

Abstract

The invention provides an improved flow cytometer based on an optical fiber integrated microfluidic chip. The improved flow cytometer is characterized by consisting of five modules, namely an opticalfiber integrated micro-fluidic chip, a light source, a fluid control system, a photoelectric detection system and a waste liquid collection system, in the system, the fluid control system controls a cell sample and sheath fluid to form a cell flow, the cell flow flows into a cell flow conduit of a micro-fluidic chip, the cell flow conduit is connected with a hollow annular core optical fiber, andcells in the cell flow are irradiated by a self-focusing light beam of the hollow annular core optical fiber to generate scattered light. Scattered light collecting optical fibers are embedded in themicro-fluidic chip and are distributed on two sides of the micro-fluidic channel. Scattered signal light in different directions is collected by the optical fiber and transmitted to the photoelectricdetection system for light splitting detection. The improved flow cytometer can be used for analyzing cells.

Description

[0001] (1) Technical field [0002] The invention relates to an improved flow cytometer based on an optical fiber integrated microfluidic chip, which can be used for automatic cell analysis and sorting, and belongs to the technical field of cell detection and analysis. [0003] (2) Background technology [0004] The working principle of flow cytometry is to conduct multi-parameter and rapid quantitative analysis of single cells or other biological particles through monoclonal antibodies at the molecular level. It can analyze tens of thousands of cells at high speed, and can measure multiple parameters from one cell at the same time. It has the advantages of high speed, high precision and good accuracy. It is one of the most advanced cell quantitative analysis technologies in the contemporary era. First used in the 1950s to measure cell volume, it detects cells as they pass in a straight line with a fast-flowing fluid through an observation hole. In a flow cytometer, cells in s...

AI Technical Summary

Problems solved by technology

They are: flow chamber and liquid flow system; laser source and optical system; photoelectric cell and detection system; computer and analysis system. Module independence, its optical path and space structure are extremely complex, and the manufacturing cost is relatively high

Patent CN111024592A discloses an optical path device of a flow cytometer, the optical path volume is relatively large, and the light receiving structure is relatively complicated

[0007] The traditional flow cytometer uses a spatial optical path, which has high requirements on the spatiality and stability of the optical path, and requires precise adjustment of the optical path, and the optical components are greatly disturbed by external environments such as vibration, temperature, and humidity.

Moreover, these spatial optical components are bulky and the assembly method is not flexible enough

More importantly, the traditional spatial optical path system uses a microscopic objective lens as the light-receiving medium, and the objective lens is large in size, which limits the number of objective lenses used and the integration of optical paths.

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