Improved flow cytometer based on optical fiber integrated micro-fluidic chip

A flow cytometer and microfluidic chip technology, applied in the field of cell detection and analysis, can solve the problems of limiting the number of objective lenses used and optical path integration, high requirements for optical path space and stability, complex optical path and spatial structure, etc. Various methods, rich design diversity, and various effects of materials

Pending Publication Date: 2021-01-15
GUILIN UNIV OF ELECTRONIC TECH
7 Cites 0 Cited by

AI-Extracted Technical Summary

Problems solved by technology

They are: flow chamber and liquid flow system; laser source and optical system; photoelectric cell and detection system; computer and analysis system. Module independence, its optical path and space structure are extremely complex, and the manufacturing cost is relatively high
Patent CN111024592A discloses an optical path device of a flow cytometer, the optical path volume is relatively large, and the light receiving structure is relatively complicated
[0007] The traditional flow cytometer uses a spatial optical path, which has high requirements on the spatia...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Abstract

The invention provides an improved flow cytometer based on an optical fiber integrated microfluidic chip. The improved flow cytometer is characterized by consisting of five modules, namely an opticalfiber integrated micro-fluidic chip, a light source, a fluid control system, a photoelectric detection system and a waste liquid collection system, in the system, the fluid control system controls a cell sample and sheath fluid to form a cell flow, the cell flow flows into a cell flow conduit of a micro-fluidic chip, the cell flow conduit is connected with a hollow annular core optical fiber, andcells in the cell flow are irradiated by a self-focusing light beam of the hollow annular core optical fiber to generate scattered light. Scattered light collecting optical fibers are embedded in themicro-fluidic chip and are distributed on two sides of the micro-fluidic channel. Scattered signal light in different directions is collected by the optical fiber and transmitted to the photoelectricdetection system for light splitting detection. The improved flow cytometer can be used for analyzing cells.

Application Domain

Technology Topic

Image

  • Improved flow cytometer based on optical fiber integrated micro-fluidic chip
  • Improved flow cytometer based on optical fiber integrated micro-fluidic chip
  • Improved flow cytometer based on optical fiber integrated micro-fluidic chip

Examples

  • Experimental program(2)

Example Embodiment

[0034]Example 1:
[0035]Such asfigure 1 Shown is a system diagram of an improved flow cytometer based on a fiber integrated microfluidic chip. It is composed of five modules: optical fiber integrated microfluidic chip 1, light source 2, fluid control system 6, photoelectric detection system 7, and waste liquid collection system 9. In the system, the fluid control system 6 controls the cell sample and the sheath fluid to form a single cell flow, and flows into the cell flow conduit 10 of the microfluidic chip 1, and the cell flow conduit 10 is connected to the hollow annular core optical fiber 4. The hollow ring-core fiber 4 is connected to the optical fiber 3 to introduce the excitation light of the light source 2. The connection between the hollow ring-core fiber 4 and the optical fiber 3 can be in the form of a coupler, such asFigure 8 As shown in (a), it can also be in the form of core alignment, such asFigure 8 (b) Shown. The hollow ring-core optical fiber 4 uses its own hollow part 4 to 5 as a micro flow channel to allow cell flow to pass. The output end of the hollow ring core optical fiber 4 is made into a frustum structure by fiber grinding technology, which is used for focusing and irradiating the excitation light. The cells 11 in the cell flow are due to the self-focusing effect of the beam at the output end of the hollow ring core optical fiber 4 , Only one can pass at a time at the autofocus, as shown in Figure 8. After the cells 11 in the cell stream are irradiated by the excitation light source, they are collected and processed by the waste liquid collection system 9. The scattered light generated by the irradiation is collected by scattered light collection fibers 8-1, 8-2, and transmitted to the photoelectric detection system 7-1, 7-2 for corresponding detection and analysis.
[0036]PE-TxRed was selected as the fluorescent marker of the cell sample, and the excitation light source wavelength was 488nm. After the PE-TxRed attached to the cells in the single-cell flow is irradiated by the excitation light source, the generated scattered excitation light has a wavelength of 620nm. The scattered light is collected by the scattered light collection fibers 8-1 and 8-2 and transmitted to the photoelectric detection system 7 respectively. -1, 7-2. Take the photoelectric detection system 7-2 as an example to illustrate the scattering and detection process of scattered light.
[0037]The scattered light collecting fiber 8-2 collects scattered light of two wavelengths, namely: the excitation light of the light source with a wavelength of 488nm and the excited light of 620nm. The scattered light collection optical fiber 8-2 transmits the collected scattered light to the photodetection system 7-2. The photodetection system 7-2 contains a spectroscopic module 12 and a photodetection module 13. The beam splitting surfaces of the beam splitters 12-1 and 12-2 of the beam splitting module 12 are respectively plated with 500nm long-wave pass dichroic mirrors and short-wave pass dichroic mirrors. Correspondingly, the single photon detectors 13-1 and 13-2 in the photoelectric detection module 13 are used to detect scattered light with a wavelength of 488 nm and scattered light with a wavelength of 620 nm, respectively.

Example Embodiment

[0038]Example 2:
[0039]FITC and APC-Cy7 were selected as the markers of the cells in the single-cell flow. The excitation light wavelengths were 488nm and 635nm, and the excited light wavelengths were 530nm and 780nm.
[0040]The light sources 2-1 and 2-2 are laser light sources of 488 nm and 635 nm, respectively, which are combined by the wavelength division multiplexer 14 and then transmitted to the hollow ring core fiber 4 via the optical fiber 3. The cells 11 in the cell flow generate scattered light after being irradiated, and the scattered light is collected by the scattered light collecting fibers 8-1, 8-2, and transmitted to the photoelectric detection systems 7-1, 7-2, respectively. The scattered light contains a total of 4 wavelengths of light, the excitation light with wavelengths of 488nm and 635nm, and the excited light with wavelengths of 530nm and 780nm. Take the photoelectric detection system 7-2 as an example to illustrate the spectroscopy and detection of scattered light.
[0041]There are four wavelengths of scattered light entering the photoelectric detection system. The light splitting surface of the light splitting interface 12-1 is coated with a short-wavelength pass dichroic mirror with a wavelength of 700nm. The scattered light with a wavelength of 780 enters the photoelectric detection module 13 first. The photon detector 13-1 carries out the corresponding detection processing. The spectroscopic surface of the spectroscopic interface 12-2 is coated with a short-wavelength pass dichroic mirror with a spectroscopic wavelength of 600nm, and the wavelength of 635nm enters the photodetector module 13 through the spectroscopic interface 12-2. , The single-photon detector 13-2 carries out corresponding detection processing, and the spectroscopic surface of the spectroscopic interface 12-3 is coated with a short-wavelength pass dichroic mirror with a wavelength of 500nm, and the scattered light with a wavelength of 530nm enters through the spectroscopic interface 12-3 The photodetection module 13 is processed by the single-photon detector 13-3. Finally, the light-splitting interface of the light-splitting interface 12-4 is coated with a long-wavelength pass dichroic mirror with a wavelength of 500nm, and the scattered light with a wavelength of 488nm passes through the light-splitting interface 12-4 enters the photodetection module 13, and the single-photon detector 13-4 performs corresponding detection processing.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Similar technology patents

Self-repair material and article with shape memory effect and preparation method thereof

InactiveCN106633721ARich preparation methodImprove reliabilitySurgeryPharmaceutical delivery mechanismSelf-healing materialMemory functions
Owner:SHENZHEN INST OF ADVANCED TECH

Method for preparing gradient material by stirring friction welding and prepared gradient material

ActiveCN109570933ARich preparation methodRealizing continuous gradient materialsNon-electric welding apparatusFriction stir weldingEnergy consumption
Owner:XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY

Application of traditional Chinese medicine composite in treating rabies

InactiveCN104984170AReasonable drug formulationRich preparation methodAntiviralsPlant ingredientsCarthamusLycopodium clavatum spore
Owner:庄立

Corn deep-processing method and prepared product cooking method

InactiveCN104872520ARich preparation methodRich varietyFood preparationEngineeringCooking methods
Owner:XINMIN JULIUHE VILLAGE ZHONGWEI CORN PLANTING PROFESSIONAL COOP

Classification and recommendation of technical efficacy words

  • Rich preparation method

Self-repair material and article with shape memory effect and preparation method thereof

InactiveCN106633721ARich preparation methodImprove reliabilitySurgeryPharmaceutical delivery mechanismSelf-healing materialMemory functions
Owner:SHENZHEN INST OF ADVANCED TECH

Corn deep-processing method and prepared product cooking method

InactiveCN104872520ARich preparation methodRich varietyFood preparationEngineeringCooking methods
Owner:XINMIN JULIUHE VILLAGE ZHONGWEI CORN PLANTING PROFESSIONAL COOP

Method for preparing gradient material by stirring friction welding and prepared gradient material

ActiveCN109570933ARich preparation methodRealizing continuous gradient materialsNon-electric welding apparatusFriction stir weldingEnergy consumption
Owner:XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY

Application of traditional Chinese medicine composite in treating rabies

InactiveCN104984170AReasonable drug formulationRich preparation methodAntiviralsPlant ingredientsCarthamusLycopodium clavatum spore
Owner:庄立
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products