Improved flow cytometer based on optical fiber integrated micro-fluidic chip

A flow cytometer and microfluidic chip technology, applied in the field of cell detection and analysis, can solve the problems of limiting the number of objective lenses used and optical path integration, high requirements for optical path space and stability, complex optical path and spatial structure, etc. Various methods, rich design diversity, and various effects of materials

Pending Publication Date: 2021-01-15
GUILIN UNIV OF ELECTRONIC TECH
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  • Application Information

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Problems solved by technology

They are: flow chamber and liquid flow system; laser source and optical system; photoelectric cell and detection system; computer and analysis system. Module independence, its optical path and space structure are extremely complex, and the manufacturing cost is relatively high
Patent CN111024592A discloses an optical path device of a flow cytometer, the optical path volume is relatively large, and the light receiving structure is relatively complicated
[0007] The traditional flow cytometer uses a spatial optical path, which has high requirements on the spatia

Method used

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  • Improved flow cytometer based on optical fiber integrated micro-fluidic chip
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  • Improved flow cytometer based on optical fiber integrated micro-fluidic chip

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Example Embodiment

[0034]Example 1:

[0035]Such asfigure 1 Shown is a system diagram of an improved flow cytometer based on a fiber integrated microfluidic chip. It is composed of five modules: optical fiber integrated microfluidic chip 1, light source 2, fluid control system 6, photoelectric detection system 7, and waste liquid collection system 9. In the system, the fluid control system 6 controls the cell sample and the sheath fluid to form a single cell flow, and flows into the cell flow conduit 10 of the microfluidic chip 1, and the cell flow conduit 10 is connected to the hollow annular core optical fiber 4. The hollow ring-core fiber 4 is connected to the optical fiber 3 to introduce the excitation light of the light source 2. The connection between the hollow ring-core fiber 4 and the optical fiber 3 can be in the form of a coupler, such asFigure 8 As shown in (a), it can also be in the form of core alignment, such asFigure 8 (b) Shown. The hollow ring-core optical fiber 4 uses its own hollow pa...

Example Embodiment

[0038]Example 2:

[0039]FITC and APC-Cy7 were selected as the markers of the cells in the single-cell flow. The excitation light wavelengths were 488nm and 635nm, and the excited light wavelengths were 530nm and 780nm.

[0040]The light sources 2-1 and 2-2 are laser light sources of 488 nm and 635 nm, respectively, which are combined by the wavelength division multiplexer 14 and then transmitted to the hollow ring core fiber 4 via the optical fiber 3. The cells 11 in the cell flow generate scattered light after being irradiated, and the scattered light is collected by the scattered light collecting fibers 8-1, 8-2, and transmitted to the photoelectric detection systems 7-1, 7-2, respectively. The scattered light contains a total of 4 wavelengths of light, the excitation light with wavelengths of 488nm and 635nm, and the excited light with wavelengths of 530nm and 780nm. Take the photoelectric detection system 7-2 as an example to illustrate the spectroscopy and detection of scattered lig...

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Abstract

The invention provides an improved flow cytometer based on an optical fiber integrated microfluidic chip. The improved flow cytometer is characterized by consisting of five modules, namely an opticalfiber integrated micro-fluidic chip, a light source, a fluid control system, a photoelectric detection system and a waste liquid collection system, in the system, the fluid control system controls a cell sample and sheath fluid to form a cell flow, the cell flow flows into a cell flow conduit of a micro-fluidic chip, the cell flow conduit is connected with a hollow annular core optical fiber, andcells in the cell flow are irradiated by a self-focusing light beam of the hollow annular core optical fiber to generate scattered light. Scattered light collecting optical fibers are embedded in themicro-fluidic chip and are distributed on two sides of the micro-fluidic channel. Scattered signal light in different directions is collected by the optical fiber and transmitted to the photoelectricdetection system for light splitting detection. The improved flow cytometer can be used for analyzing cells.

Description

[0001] (1) Technical field [0002] The invention relates to an improved flow cytometer based on an optical fiber integrated microfluidic chip, which can be used for automatic cell analysis and sorting, and belongs to the technical field of cell detection and analysis. [0003] (2) Background technology [0004] The working principle of flow cytometry is to conduct multi-parameter and rapid quantitative analysis of single cells or other biological particles through monoclonal antibodies at the molecular level. It can analyze tens of thousands of cells at high speed, and can measure multiple parameters from one cell at the same time. It has the advantages of high speed, high precision and good accuracy. It is one of the most advanced cell quantitative analysis technologies in the contemporary era. First used in the 1950s to measure cell volume, it detects cells as they pass in a straight line with a fast-flowing fluid through an observation hole. In a flow cytometer, cells in s...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1434G01N2015/144
Inventor 苑立波朱立夫
Owner GUILIN UNIV OF ELECTRONIC TECH
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