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Application of aronia juice in inhibition of skin aging

A technology of aronia and uses, applied in the field of aronia juice, can solve problems such as accelerated skin aging

Pending Publication Date: 2021-01-19
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, certain other environmental factors can accelerate the aging process of the skin

Method used

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  • Application of aronia juice in inhibition of skin aging
  • Application of aronia juice in inhibition of skin aging
  • Application of aronia juice in inhibition of skin aging

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0055] [Example 1] Cell experiment - aronia juice inhibits ROS generation

[0056] Here, the fluorescent probe DCFH-DA was used with flow cytometry to measure the changes in the content of reactive oxygen species in human skin fibroblasts CCD-966sk treated with aronia juice.

[0057] Materials and Instruments

[0058] 1. Cell line: human skin fibroblast (Human skin fibroblast) CCD-966sk (purchased from Biological Resource Conservation and Research Center (BCRC), No. 60153).

[0059] 2. Culture medium: Eagle's minimum essential medium (minimum essential medium, MEM, purchased from Gibco, 15188-319) was added with additional components to make it contain 10vol% FBS (fetal bovine Serum, purchased from Gibco, 10437-028), 90vol% 1mM sodium pyruvate (sodium pyruvate, purchased from Gibco, 11360-070), 1.5g / L sodium bicarbonate (purchased from Sigma), 0.1mM non-essential amino acid solution (purchased from Gibco, 11140-050).

[0060] 3. Phosphate buffered saline solution (PBS solutio...

example 2

[0086] [Example 2] Cell experiment - aronia juice reduces melanin production

[0087] Herein, an ELISA reader (enzyme-linked immunosorbent assay reader) was used to measure the changes in the melanin content of the melanoma cell line B16F10 treated with aronia juice.

[0088] Materials and Instruments

[0089] 1. Cell line: mouse melanoma cell line B16F10 (ATCC CRL-6475), purchased from American Type Culture Collection (ATCC).

[0090] 2. Culture medium: Dulbecco's modified minimal essential medium (DMEM, purchased from Gibco) was added with additional components to make it contain 1vol% of antibiotic solution (Antibiotic AntimycoticSolution, purchased from Gibco, 15240-062), 10vol% of FBS (fetal bovine Serum , available from Gibco, 10437-028).

[0091] 3. Phosphate buffered saline solution (PBS solution): purchased from Gibco, product number 10437-028.

[0092] 4. Prepare 1N NaOH (purchased from Sigma, product number 221465) solution with double distilled water.

[0093] ...

example 3

[0118] [Example 3] Cell experiment - aronia juice promotes skin fibroblast proliferation

[0119] By Click-iT TM The EdU method (by means of EdU treatment of cells, fixation and permeabilization of cells, and detection of a fluorescent dye combined with Edu) was used to detect the number of cells, thereby checking whether aronia juice has a pro-proliferative effect on fibroblasts.

[0120] Materials and Instruments

[0121] 1. Cell line: Human skin fibroblast CCD-966SK, purchased from American Type Culture Collection (ATCC).

[0122] 2. Culture medium: Eagle's minimum essential medium (minimum essential medium, MEM, purchased from Gibco, 15188-319) was added with additional components to make it contain 10vol% FBS (fetal bovine Serum, purchased from Gibco, 10437-028), 90vol% 1mM sodium pyruvate (sodium pyruvate, purchased from Gibco, 11360-070), 1.5g / L sodium bicarbonate (purchased from Sigma), 0.1mM non-essential amino acid solution (purchased from Gibco, 11140-050).

[0...

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Abstract

The invention provides application of aronia juice in a composition for inhibiting skin aging. The composition is used for inhibiting melanogenesis, inhibiting the generation of reactive oxygen species and promoting the proliferation of skin fibroblast.

Description

technical field [0001] This case concerns the use of an aronia juice. Especially the use of aronia juice for the preparation of compositions for preventing skin aging. Background technique [0002] Skin provides the first-stage protection for human individuals against environmental factors such as ultraviolet (ultraviolet, UV) radiation in sunlight, pathogens, and friction. The skin consists of the epidermis, the dermis, which is mainly composed of connective tissue, and the subcutaneous tissue, from the outside to the inside. Among them, the dermis contains sufficient molecules such as collagen, elastin, and hyaluronic acid, so young skin has better support and elasticity. [0003] Skin naturally ages normally with age, resulting in various damaged skin conditions. For example, due to the influence of reactive oxygen species (reactive oxygen species, ROS, also known as free radicals) in the environment, the collagen in the skin is cracked, which leads to decreased skin e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A61K8/9789A61Q19/00A61Q19/08A61Q17/04
CPCA23L33/105A61K8/9789A61Q19/00A61Q19/08A61Q17/04A61Q19/004A23V2002/00A23V2200/318A23V2250/21
Inventor 林咏翔姚采涵
Owner 百岳特生物技术(上海)有限公司