Single cell encapsulation via pickering emulsion for bio-pesticides application
A single unit, a single technology, applied in the field of Pickering emulsion and single unit packaging, can solve the problem of toxic chemicals and compounds
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[0070] The following examples are not intended to limit the scope of the claims in any way. The following examples are presented to provide those of ordinary skill in the art with a complete disclosure and description of how to make and use the described invention, and are not intended to limit the scope of the invention, nor are they intended to represent all or only the experiments performed below. Unless indicated otherwise, parts are parts by weight, molecular weight is weight average molecular weight, temperature is in degrees Celsius, and pressure is at or near atmospheric.
example 1
[0071] Example 1 - Salification of a silica surface using APTES
[0072] Silica (1 g) (AEROSIL OX 50, estimated primary particle size 40 nm, obtained from Evonik, Germany) was added to 40 mL of methanol and stirred to completely disperse. After that, APTES (99% Sigma-Aldrich) was slowly added to the solution to a final concentration of 0.5M. The reaction was carried out at ambient temperature for 45 minutes. After silanization, the particles were collected by centrifugation (9000 rpm, 10 minutes) and washed four times with methanol. Thereafter, the silica-NH 2 The nanoparticles were dried at 35°C under vacuum for about 3 hours.
example 2- 2
[0073] Example 2 - Silica - NH 2 Preparation of Pickering emulsion
[0074] Pickering emulsions were prepared from amine-functionalized silica in water and paraffin oil (Sigma-Aldrich, analytical grade). First, the silica-NH 2 Nanoparticles were dispersed in distilled water with increasing silica content: 0.1 wt%, 1 wt%, 2 wt%, 3 wt% and 5 wt%. Afterwards, paraffin oil was added at the ratio of 5:95, 10:90, 20:80 and 30:70 volume % o / w, respectively. The mixture was emulsified by sonication for 5 minutes. Figure 2A Confocal microscope images (a to d) are provided showing 1% silica-NH at different oil / water ratios 2 Pickering lotion. Figure 2A Part (a) of the figure shows an oil / water ratio of 5:95. Figure 2A Part (b) of the figure shows an oil / water ratio of 10:90. Figure 2A Part (c) of the figure shows an oil / water ratio of 20:80. Figure 2A Part (d) of the figure indicates an oil / water ratio of 30:70. The ratio of oil / water can also be: 1:99, 40:60 and 50:50.
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