Ganoderma applanatum specimen preparation method

A technique for making Ganoderma lucidum and specimens, which is applied in the fields of botany equipment and methods, applications, and plant preservation, and can solve problems such as large size, perishability, discoloration, and potential safety hazards of Ganoderma lucidum, and achieve safety and protection. Good authenticity, good anti-corrosion and moisture-proof performance, good inhibitory effect

Active Publication Date: 2021-01-29
GUANGXI NORMAL UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Ganoderma lucidum is large and hard, and the immature fungi are easy to rot and change color when stored under natural conditions after picking, and then lose their natural and beautiful colors, and ordinary drying methods cannot retain the original color and shape of the fungus , is not conducive to the preservation and identification of specimens
In the traditional method, there is also the method of soaking in formalin to prepare specimens, but this method not only uses toxic liquid for soaking, but also the color of the fungal fruiting body after soaking changes greatly, which is not conducive to the observation and identification
Formalin is 35~40% formaldehyde aqueous solution. Formaldehyde is a highly irritating and toxic gas, which is flammable and corrosive. It can be detected in a small amount in the general air and is easily soluble in water. Therefore, Formaldehyde is used. The specimens made by Marin have great potential safety hazards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of Ganoderma lucidum specimen, comprising the following steps:

[0034] S1. Collect fresh wild Ganoderma lucidum to remove surface impurities;

[0035] S2. Cleaning and pretreatment: first rinse with tap water 3 times, and then soak with cleaning solution. The cleaning solution is a combination of sodium chloride and ethanol, and the soaking time is 40 minutes. The mass concentration of the sodium chloride aqueous solution is 0.9%, the volume concentration of the ethanol aqueous solution is 75%; after the immersion is completed, add acetic acid to soak and soften, and the soak time is 2min. After the soak is completed, take out the fruiting body of Ganoderma lucidum;

[0036] S3. Carry out transverse section and longitudinal section with a sharp and hard cutting knife to the ganoderma ganoderma fruiting body, so that the fungus flesh and other structures of the fruiting body are exposed;

[0037] S4. prepare antiseptic liquid, described antiseptic ...

Embodiment 2

[0042] This embodiment is basically the same as Example 1, but the cleaning liquid in step S2 is an aqueous solution of sodium chloride with a mass concentration of 0.9% and an aqueous ethanol solution with a volume concentration of 75%, and the fruiting bodies of Ganoderma lucidum are soaked separately in sequence, Washing time is 20min and 20min respectively.

Embodiment 3

[0044]This embodiment is basically the same as Example 1, but the cleaning solution in step S2 is a combination solution of sodium chloride and ethanol, and the immersion time is 40min, wherein the mass concentration of the sodium chloride aqueous solution is 2%, and the volume concentration of the ethanol aqueous solution 95%.

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PUM

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Abstract

The invention discloses a Ganoderma applanatum specimen preparation method, which comprises the following steps: cleaning, transversely cutting and longitudinally cutting, soaking in preservative solution consisting of star anise cold extracting solution and winged bean hot extracting solution, freeze-drying, and attaching a UV adhesive layer. The preparation method has characteristics of good safety and fidelity, good anti-corrosion and damp-proof effects and long preservation time.

Description

technical field [0001] The invention relates to the technical field of specimen preparation, in particular to a method for preparing a Ganoderma ganoderma specimen. Background technique [0002] Ganoderma lucidum is also known as Ganoderma lucidum, old mother fungus, and maple fungus. The fruiting body is large or extra large, sessile or almost sessile; the cap is semicircular, oblate hemispherical or flat, with gray surface and gradually brown. There are concentric ring ribs, sometimes with tumors, the leather shell is horny, and the edges are thinner; the flesh of the bacteria is light chestnut, and the pores are round. It is one of the important wood rot fungi, which can cause white rot in the xylem of wood, and can be used as medicine. It is used as an anti-cancer drug in China and Japan, and it can also treat rheumatic tuberculosis. [0003] Ganoderma lucidum is a fungal molecule in the ecology of nature. Together with many fungi, it plays a pioneering and leading rol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 巫桂芬农万廷
Owner GUANGXI NORMAL UNIV FOR NATITIES
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