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25-hydroxy vitamin D calibrator matrix, preparation method and application

A technology of hydroxyvitamins and calibrator, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of insufficient accuracy of test results and achieve the effect of high accuracy

Pending Publication Date: 2021-02-02
GUANGZHOU KINGMED DIAGNOSTICS CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Based on this, it is necessary to provide a 25-hydroxyvitamin D calibrator matrix for the problem of insufficient accuracy of the detection results in the process of using the existing calibrator matrix to detect 25-hydroxyvitamin D by liquid chromatography tandem mass spectrometry , preparation method, calibrator and kit

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  • 25-hydroxy vitamin D calibrator matrix, preparation method and application
  • 25-hydroxy vitamin D calibrator matrix, preparation method and application
  • 25-hydroxy vitamin D calibrator matrix, preparation method and application

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preparation example Construction

[0040] The preparation method described in the embodiment of the present invention is simple to prepare and low in cost; by adding a small amount of additives, the detection result of 25-hydroxyvitamin D is more accurate when liquid chromatography tandem mass spectrometry is used.

[0041] In one embodiment, removing the vitamin D impurities in the bovine serum albumin before the mixing includes: after dissolving the bovine serum albumin, adding activated carbon for stirring, and then removing the activated carbon.

[0042] There may be vitamin D interference in the commercially available bovine serum albumin. Therefore, the embodiment of the present invention performs impurity removal treatment on the bovine serum albumin with vitamin D interference. Thereby further ensuring the accuracy of the detection results.

[0043] In one embodiment, in the preparation method, the additive is mixed with the bovine serum albumin and PBS buffer in the form of a stock solution, and the st...

Embodiment 1

[0051] (1) Weigh 8.95 g of disodium hydrogen phosphate dodecahydrate with a balance, dissolve it in 1 L of deionized water, and adjust the pH to 7.5 with 85% phosphoric acid to obtain a PBS buffer solution.

[0052] (2) Weigh 40.00 g of bovine serum albumin with a balance and add it to the PBS buffer in step (1) to obtain a bovine serum albumin solution.

[0053] (3) Pipette 1 mL of an ethanol solution of estriol with a concentration of 1.0 mg / mL, add it to the bovine serum albumin solution in step (2), and mix evenly to obtain a calibrator matrix.

Embodiment 2

[0055] (1) Weigh 8.95 g of disodium hydrogen phosphate dodecahydrate with a balance, dissolve it in 1 L of deionized water, and adjust the pH to 7.5 with 85% phosphoric acid to obtain a PBS buffer solution.

[0056] (2) Weigh 40.00 g of bovine serum albumin with a balance and add it to the PBS buffer in step (1) to obtain a bovine serum albumin solution.

[0057] (3) The bovine serum albumin solution obtained in step (2) is processed according to the detection process of 25-hydroxyvitamin D, detects the background, finds that it has vitamin D interference, and adds 60g of activated carbon to the bovine serum albumin solution , magnetically stirred for 4 hours, and then filtered or centrifuged to remove activated carbon to obtain a bovine serum albumin solution after removal of impurities.

[0058] (4) Pipette 1 mL of an ethanol solution of estriol with a concentration of 1.0 mg / mL, add it to the bovine serum albumin solution after the impurity removal described in step (3), an...

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Abstract

The invention discloses a 25-hydroxy vitamin D calibrator matrix which is used for detecting 25-hydroxy vitamin D in human serum by liquid chromatography-tandem mass spectrometry, and comprises bovineserum albumin, a PBS buffer solution and an additive, the PBS buffer solution comprises phosphate and water, and the additive is selected from one or more of estradiol, estriol, and rostenediol and cortisol. The invention further discloses a preparation method and application of the calibrator matrix. The application comprises a calibrator and a kit. In the calibrator matrix, due to the additionof the additive, the accuracy of a detection result is higher when the 25-hydroxy vitamin D is detected by using a liquid chromatography-tandem mass spectrometry method.

Description

technical field [0001] The present invention relates to the technical field of 25-hydroxyvitamin D detection, in particular to a calibrator matrix, a preparation method and an application. The application includes a calibrator and a kit, and the calibrator matrix is ​​specifically used for liquid chromatography tandem mass spectrometry Determination of 25-hydroxyvitamin D in human serum. Background technique [0002] Vitamin D is a steroid derivative, mainly including vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol), and its main physiological role is to maintain the metabolic balance of human calcium and promote bone formation. In addition, vitamin D plays an important role in anti-tumor activity and immune function regulation, and the occurrence of various chronic diseases (such as dementia, arteriosclerosis, hypertension, coronary heart disease, diabetes, breast cancer, obesity, migraine attack frequency, etc.) Associated with abnormal vitamin D levels. 25-H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/14G01N30/86G01N30/88
CPCG01N30/02G01N30/72G01N30/04G01N30/06G01N30/14G01N30/8665G01N30/88G01N2030/045G01N2030/8822
Inventor 赵蓓蓓李冰玲程雅婷佘旭辉董衡
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT