Method for analyzing transcription and translation activity of functional genes of microbial community

Abstract

The invention discloses a method for analyzing the transcription and translation activity of functional genes of a microbial community. The method comprises the following steps: extracting a total DNAsample, total RNA sample and total protein sample of the microbial community; carrying out metagenome sequencing, metatranscriptome sequencing and meta-protrinology analysis on the extracted samples;constructing a microbial community functional gene coded protein database by utilizing a metagenome sequencing result; analyzing the transcription activity of the functional genes of the microbial community on the basis of the protein database and an mRNA sequence data set generated by metatranscriptome sequencing; and analyzing the translation activity of the functional genes of the microbial community on the basis of a meta-protrinology analysis result and the protein database. According to the method, the unified microbial community functional genome protein database is constructed, and the expression quantities of transcription and translation of the functional genes of the community are analyzed according to the database; the differences of the expression levels of transcription andtranslation of the functional genes of the community are directly compared, thereby researching the post-transcriptional control process of the functional genes of the community.

Description

technical field [0001] The invention belongs to the technical field of bioinformatics, and in particular relates to a method for analyzing the transcription and translation activities of functional genes of microbial communities. Background technique [0002] Microbial communities are widely distributed in all ecosystems on the earth and play an important role in the global material and energy cycle. Therefore, the function and activity of microbial communities have attracted the attention of researchers. Traditional techniques based on pure culture can analyze less than 1% of the total microorganisms in nature, so traditional techniques cannot fully answer the question of how microbial communities function. [0003] With the development of bioinformatics technology, the use of metagenomics, metatranscriptomics and metaproteomics to study the gene function and regulatory process of microbial communities has become a new technical hotspot. However, metagenomics can analyze t...

AI Technical Summary

Problems solved by technology

However, metagenomics can analyze the genes and potential functions contained in microbial communities through metagenomic sequencing, but it cannot reveal the dynamic expression of community genes and the post-transcriptional regulation of genes.

Both metatranscriptomics and metaproteomics can calculate the expression activity of microbial community gene functions, but the current methods for constructing functional genomic protein databases of microbial communities are not uniform

One is to construct a database using only metagenomic sequencing data. This method is difficult to fully cover the functional genes of the community, which may easily lead to incomplete calculation of the activity of the functional genes of the community; the other is to use the National Center for Biotechnology Information (National Center For Biotechnology Information (NCBI) database, the method of constructing a database of bacterial genome maps of similar communities, this method cannot accurately construct a protein database, resulting in inaccurate calculation of community functional activity

Therefore, the current technology cannot directly compare the expression levels of transcription and translation, resulting in the inability to understand the post-transcriptional regulation of functional genes in microbial communities and the mechanism of maintaining functional homeostasis

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