Method for identifying marker of candidate gene related to milk production traits of buffaloes and application

A candidate gene, buffalo technology, applied in the directions of genomics, biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of improving reliability
CN112342302AActive Publication Date: 2021-02-09GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
Publication Date
2021-02-09

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Abstract

The invention relates to the technical field of molecular markers, and discloses a method for identifying a molecular marker of a candidate gene related to milk production traits of buffaloes. The method comprises the following specific steps: (1) obtaining SNP genotypes of 45 buffalo individuals by a dd-RAD method; (2) performing grouping based on population genetic diversity and population structures, further performing selection signal analysis by using Fst and XP-CLR strategies, and identifying the candidate gene related to the milk production traits; (3) using a comparative transcriptomestrategy to assist in verifying the relevance between the candidate gene and the milk production traits of the buffaloes; and (4) identifying the related molecular marker influencing the milk production traits of the buffaloes by using a marker-trait correlation analysis strategy. The candidate gene SCLO3A1 related to the milk production traits of the buffaloes and the molecular marker g.54621870G>T influencing the milk protein rate are identified by the method for the first time. The molecular marker can be used for breeding a buffalo population with high milk protein content.
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Description

【Technical Field】

[0001] The invention relates to the field of biotechnology, in particular to a method and application for identifying candidate gene markers for buffalo milk production traits.【Background technique】

[0002] Water buffalo is an important special dairy species in southern my country. It has excellent milk quality and rich nutrition. It has the reputation of "king of milk" and is deeply loved by consumers. Water buffalo can be divided into two subspecies, river-type and marsh-type buffalo. Among them, river-type buffalo is mainly used for both milk and meat, which is the representative of high-yielding buffalo group, and swamp-type buffalo is mainly used for labor, which is the representative of low-yield buffalo group. However, low milk production performance has become a major scientific issue affecting the development of the buffalo industry. Exploring the causal genes related to the milk production traits of buffaloes has become a hotspot of scientific research on buff...

Claims

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