Method for verifying endophytic symbiotic bacteria
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A technology of symbiotic bacteria and strains, applied in the field of plant pathology
Inactive Publication Date: 2021-02-12
钱康(北京)科技有限公司
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At present, traditional methods cannot well prove the endosymbiotic technology that certain microorganisms can colonize, reproduce, and transfer in plants, so it is extremely important to verify the existence of endosymbiotic bacteria in organisms
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Embodiment 1
[0047] Examples of validation of the Endosymbiotic Bacillus strain M-33:
[0048] The M-33 strain in Bacillus was calibrated with radioactive cobalt 60, and at the same time, it was bred into a strain resistant to streptomycin and rifampicin;
[0049] The marked M-33 strain is inoculated on the roots of the cultivated rice seedlings, and placed in a verified endophytic symbiosis device, which is strictly sealed. It is impossible for the M-33 strain to colonize, reproduce, and transfer on the surface of rice seedlings. The M-33 strain can only be colonized, propagated, and transferred in rice. Within 10 days, in the rice seedling cell tissue, the tested strain M-33 can exist from the top leaves of rice seedlings, proving that the endophytic symbiotic strain M-33 is indeed present in the rice cell tissue, proving that the M-33 strain It is indeed colonization, propagation and transfer in rice seedlings. Completely autonomous transfer of the rhizome to the top stem and leaf;
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Embodiment 2
[0052] Validation of endosymbiotic Bacillus M-33 against cotton wilt:
[0053] The M-33 strain of endosymbiotic Bacillus with radioactive double resistance is used to control cotton wilt.
[0054] When the radiation double antibacterial strain M-33 is used for cotton seed dressing, the cotton seed dressing is inoculated, and the control effect on cotton wilt can reach 55%-65%.
[0055] 1. Vaccination:
[0056] When cotton is inoculated, cotton seeds are dressed with radioactive double antibacterial strain M-33.
[0057] The tested M-33 strain colonized, multiplied and transferred in the rhizome.
[0058] Fusarium wilt is delayed for 10-15 days in the initial stage of rhizome infestation; the incidence index of vitamin bundles in rhizomes is reduced by 50%-55%.
[0059] 2. Bolling period:
[0060] Fusarium wilt in cotton, when cotton buds bloom, the fusarium wilt has expanded from the rhizome to the petiole of the boll.
[0061] At this time, inoculate (spray, dip) the petio...
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Abstract
The invention discloses a method for verifying endophytic symbiotic bacteria. The method comprises the following step that S1, devices for verifying the endophytic symbiotic bacteria are provided, wherein basic devices comprise glass wide-mouth bottles which are provided with glass bell jars, and a plastic bottle plug is placed at a bottle opening of each glass wide-mouth bottle; standby materialscomprise vaseline and other substances capable of tightly sealing the bottle openings of the glass wide-mouth bottles; and a drying machine is used for exhausting air in the bell jar. According to the method, microorganisms come from an organism natural ecological system rather than the category of soil microorganisms, tested microorganisms are marked through various biological, physical and chemical methods, and interference such as pollution and drifting possibly generated by general radioactive interference, antibiotic marks and gene marks is completely eliminated; and it is proved that the tested microorganisms are completely and naturally planted, propagated and transferred in a living body, the microorganisms are labeled with radioactive substances, the microorganisms are cultivatedat the same time to have antagonistic performance on two or more antibiotics, and existence of the endophytic symbiotic bacteria in the living body is verified.
Description
technical field [0001] The invention relates to the technical field of plant pathology, in particular to a method for verifying endophytic symbiotic bacteria. Background technique [0002] In phytopathology, "endobiotic" is a professional term, which means that pathogenic bacteria are endophytic bacteria in plants. Some pathogenic bacteria establish a "symbiotic" relationship with plants (symbiotic), and there is no direct experiment to prove endophytic symbiosis. [0003] A biological entity is composed of individual cells, tissues and microorganisms in the body. These microorganisms can be divided into environmental microorganisms, endophytic microorganisms and endosymbiotic microorganisms. At present, traditional methods cannot well prove the endosymbiotic technology that certain microorganisms can colonize, reproduce, and transfer in plants. Therefore, it is extremely important to verify the existence of endosymbiotic bacteria in organisms. [0004] To this end, a met...
Claims
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