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Plant exosome microcapsule embedded freeze-dried powder and preparation method thereof

A technology of exosomes and freeze-dried powder, applied in the biological field, can solve the problems of easy rupture, uneven particle size, and easy clustering of microcapsules.

Inactive Publication Date: 2021-02-12
陕西佰瑞衡健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many preparation methods for microcapsules, and inappropriate selection of wall materials and incorrect embedding methods will cause disadvantages such as easy clustering, easy breakage, and uneven particle size of microcapsules. Therefore, the selection of appropriate wall materials and embedding methods has become a key research direction
The method and technology of protecting exosomes with microcapsule embedding technology has not been applied yet, and further research and exploration are needed

Method used

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  • Plant exosome microcapsule embedded freeze-dried powder and preparation method thereof
  • Plant exosome microcapsule embedded freeze-dried powder and preparation method thereof
  • Plant exosome microcapsule embedded freeze-dried powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides a plant exosome microencapsulated freeze-dried powder, including plant exosomes, a primary coating and a secondary coating; the primary coating is wrapped on the surface of the plant exosomes, and the The secondary coating is wrapped on the surface of the primary coating; the components of the primary coating are selected from alginate; the components of the secondary coating are selected from animal cellulose.

[0037] In some embodiments, the alginate is selected from sodium alginate or calcium alginate; the animal cellulose is selected from chitosan oligosaccharide.

[0038] In some embodiments, the plant exosome microencapsulated lyophilized powder also contains a lyoprotectant; the lyoprotectant is selected from resistant dextrins.

[0039]In some embodiments, the plant exosomes are selected from ginger exosomes, garlic exosomes, scallion exosomes, onion exosomes, grapefruit exosomes, broccoli exosomes, cucumber exosomes , turmeric exosome...

Embodiment 2

[0042] This embodiment provides a preparation method of ginger exosome microcapsule-embedded freeze-dried powder, which specifically includes the following steps:

[0043] Weigh 150g of fresh ginger and pour it into a juice extractor, add 150ml of sterilized PBS therein, after squeezing the juice and homogenate, centrifuge at 3000g, 4°C for 20min, to remove larger residues; take the yellow transparent solution of the middle layer in 10000g, 4 Centrifuge at 120,000g for 70 minutes at 4°C for 60 minutes, then use 1ml of sterile PBS to resuspend the pellet and then ultracentrifuge at 120,000g at 4°C for 60 minutes; after centrifugation, resuspend the pellet with 1ml of sterile PBS. The ginger exosome extract was obtained.

[0044] The above-mentioned ginger exosome extract, sterile sodium alginate solution with a concentration of 30 mg / mL and sterile soybean oil containing 2 μL / mL Tween 80 were mixed at a volume ratio of 1:1:10, using a 180 r / min magnetic force Stir for 10 min t...

Embodiment example 3

[0048] This embodiment provides a preparation method of ginger exosome microcapsule-embedded freeze-dried powder, which specifically includes the following steps:

[0049] Weigh 200g of fresh ginger and pour it into a juice extractor, add 200ml of sterilized PBS to it, squeeze the juice and homogenize it and centrifuge it at 3500g for 30min at 4°C to remove larger residues; take the yellow transparent solution of the middle layer in 15000g, 4 Centrifuge at 150,000g for 70 minutes at 4°C for 80 minutes, then use 2ml of sterile PBS to resuspend the pellet and then centrifuge at 150,000g at 4°C for 80 minutes; after centrifugation, resuspend the pellet with 2ml of sterile PBS. That is, ginger exosomes are obtained.

[0050] Take 80ml of sodium alginate solution with a mass fraction of 3% and mix it with 90ml of Ca-EDTA solution with a concentration of 25mmoL / L respectively, take 25mL of the prepared above-mentioned solution and mix it with 15mL of ginger exosomes, and add it to t...

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Abstract

The invention provides plant exosome microcapsule embedded freeze-dried powder. The plant exosome microcapsule embedded freeze-dried powder comprises a plant exosome, a primary coating and a secondarycoating; the primary coating wraps the surface of the plant exosome, and the secondary coating wraps the surface of the primary coating; the component of the primary coating is selected from alginate; the component of the secondary coating is selected from animal cellulose, and the plant exosome is subjected to micro-capsule embedding by screening a proper wall material and an embedding method, so that the concentration, activity, tolerance, targeting property and the like of the plant exosome are improved, and the plant exosome can be more widely applied to products with different matrixes.Meanwhile, the proper wall material and the embedding method do not influence the product quality and are beneficial to improve the flavor, taste and stability of the product, and especially for exosomes with bad flavor, a part of bad flavor can be covered by embedding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant exosome microcapsule-embedded freeze-dried powder and a preparation method thereof. Background technique [0002] The existing preparation method of plant exosomes is mainly through extraction and separation to make liquid for direct use, but the concentration of liquid exosomes is relatively low and sensitive to temperature, so they need to be refrigerated for storage and transportation. Products containing plant exosomes are in the The stress of environmental factors such as temperature, moisture, etc. during processing, storage, and transportation limits the application of exosomes; after drying exosomes, the influence of moisture can be overcome. The commonly used drying methods mainly include spray drying and vacuum freezing. Drying, among them, the spray drying method has low cost and high efficiency, but due to the high temperature required, it will affect the structur...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23P10/30B01J13/02
CPCA23L33/105A23P10/30B01J13/02A23V2002/00A23V2250/21A23V2200/32
Inventor 曹美霞吴丹丹苟晓会李珍珍杨琼靳莉
Owner 陕西佰瑞衡健康科技有限公司
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