Blood orange exosome microcapsule embedded freeze-dried powder and preparation method thereof

A technology of exosomes and freeze-dried powder, applied in the biological field, can solve the problems of easy clustering, easy rupture and uneven particle size of microcapsules

Inactive Publication Date: 2021-04-16
陕西佰瑞衡健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many preparation methods for microcapsules, and inappropriate selection of wall materials and incorrect embedding methods will cause disadvantages such as easy clustering, easy breakage, and uneven particle size of microcapsules. Therefore, the selection of appropriate wall materials and embedding methods has become a key research direction
The method and technology of protecting exosomes with microcapsule embedding technology has not been applied yet, and further research and exploration are needed

Method used

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  • Blood orange exosome microcapsule embedded freeze-dried powder and preparation method thereof
  • Blood orange exosome microcapsule embedded freeze-dried powder and preparation method thereof
  • Blood orange exosome microcapsule embedded freeze-dried powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides a plant exosome microencapsulated freeze-dried powder, including plant exosomes, a primary coating and a secondary coating; the primary coating is wrapped on the surface of the plant exosomes, and the The secondary coating is wrapped on the surface of the primary coating; the components of the primary coating are selected from alginate; the components of the secondary coating are selected from animal cellulose.

[0037] In some embodiments, the alginate is selected from sodium alginate or calcium alginate; the animal cellulose is selected from chitosan oligosaccharide.

[0038] In some embodiments, the plant exosome microencapsulated lyophilized powder also contains a lyoprotectant; the lyoprotectant is selected from resistant dextrins.

[0039]In some embodiments, the plant exosomes are selected from ginger exosomes, garlic exosomes, scallion exosomes, onion exosomes, grapefruit exosomes, broccoli exosomes, cucumber exosomes , turmeric exosome...

Embodiment 2

[0042] This embodiment provides a preparation method of ginger exosome microcapsule-embedded freeze-dried powder, which specifically includes the following steps:

[0043] Weigh 150g of fresh ginger and pour it into a juice extractor, add 150ml of sterilized PBS to it, and centrifuge at 3,000g for 20 minutes at 4°C to remove larger residues; take the yellow transparent solution in the middle layer at 10,000g, Centrifuge at 4°C for 70 min; take the supernatant at 120,000g, ultracentrifuge at 4°C for 60 min, then use 1ml sterile PBS to resuspend the pellet and then ultracentrifuge at 120,000g, 4°C for 60 min; after centrifugation, resuspend the pellet with 1ml sterile PBS At this time, the ginger exosome extract is obtained.

[0044] The above ginger exosome extract, sterile sodium alginate solution with a concentration of 30 mg / mL and sterile soybean oil containing 2 μL / mL Tween 80 were mixed at a volume ratio of 1:1:10, using 180 r / min magnetic stirring for 10 min to obtain ...

Embodiment example 3

[0048] This embodiment provides a preparation method of ginger exosome microcapsule-embedded freeze-dried powder, which specifically includes the following steps:

[0049] Weigh 200g of fresh ginger and pour it into a juice extractor, add 200ml of sterilized PBS to it, and centrifuge at 3500g for 30 minutes at 4°C to remove larger residues; Centrifuge at 150,000g for 70 minutes at 4°C for 80 minutes, then use 2ml of sterile PBS to resuspend the pellet and then ultracentrifuge at 150,000g at 4°C for 80 minutes; after centrifugation, resuspend the pellet with 2ml of sterile PBS, At this time, ginger exosomes are obtained.

[0050] Take 80 ml of sodium alginate solution with a mass fraction of 3% and mix it with 90 ml of Ca-EDTA solution with a concentration of 25 mmoL / L, and mix 25 mL of the prepared solution with 15 mL of ginger exosomes, and add to In 70 mL corn germ oil containing 1.2% span80, mechanically stir at 280 r / min for 20 min;

[0051] According to the molar ratio ...

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Abstract

The invention provides blood orange exosome micro-capsule embedding freeze-dried powder. The blood orange exosome micro-capsule embedding freeze-dried powder comprises plant exosome, a primary coating and a secondary coating, The primary coating wraps the surface of the plant exosome, and the secondary coating wraps the surface of the primary coating; The component of the primary coating is selected from alginate; The component of the secondary coating is selected from animal cellulose, the plant exosome is selected from orange exosome, the orange exosome is selected from sweet orange exosome, and the sweet orange exosome is selected from blood orange exosome; the blood orange exosome is subjected to micro-capsule embedding by screening a proper wall material and an embedding method, the concentration, activity, tolerance, targeting and the like of the blood orange exosome are improved, the blood orange exosome can be more widely applied to products with different matrixes, meanwhile, the proper wall material and the embedding method do not affect the product quality, and the flavor, taste and stability of the product are improved. Especially for exosomes with bad flavors, part of bad flavors can be covered by embedding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blood orange exosome microcapsule-embedded freeze-dried powder and a preparation method thereof. Background technique [0002] The existing preparation method of plant exosomes is mainly through extraction and separation to make liquid for direct use, but the concentration of liquid exosomes is relatively low and sensitive to temperature, so they need to be refrigerated for storage and transportation. Products containing plant exosomes are in the The stress of environmental factors such as temperature, moisture, etc. during processing, storage, and transportation limits the application of exosomes; after drying exosomes, the influence of moisture can be overcome. The commonly used drying methods mainly include spray drying and vacuum freezing. Drying, among them, the spray drying method has low cost and high efficiency, but due to the high temperature required, it will affect the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L19/00A23L19/10A23L25/00A23P10/30A23L29/256A23L29/275A23L29/30A23F3/26
CPCA23L33/105A23P10/30B01J13/02A23V2002/00A23V2250/21A23V2200/32
Inventor 曹美霞吴丹丹苟晓会李珍珍杨琼靳莉
Owner 陕西佰瑞衡健康科技有限公司
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