Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining content of Berol 185 in sample by using liquid chromatography-tandem mass spectrometry

A technology of liquid chromatography and tandem mass spectrometry, applied in the field of Berol185 content, to achieve the effect of simple method, less pollution and easy operation

Active Publication Date: 2021-02-12
BEIJING MINHAI BIOTECH
View PDF17 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the application of LC-MS / MS technology to the analysis or detection of Berol 185 content

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining content of Berol 185 in sample by using liquid chromatography-tandem mass spectrometry
  • Method for determining content of Berol 185 in sample by using liquid chromatography-tandem mass spectrometry
  • Method for determining content of Berol 185 in sample by using liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products. Embodiment 1 Utilizes the method for the determination of Berol 185 content in the sample by liquid chromatography tandem mass spectrometry

[0045] 1. Analytical instruments and reagents

[0046] Analytical instruments: SHIMADZU-8050 triple quadrupole mass spectrometer, equipped with ESI electrospray ion source, analysis software is Labsolutions 5.93 data processing system; Shimadzu LC20AD XR high performance liquid chromatography, equipped with Shimadzu Nexera X2 autosampler; The chromatographic column is a GL Science Bio C18 chromatographic column (100×2.1 mm, 1.7 μm).

[0047] The experimental reagents are shown in Table 1:

[0048] T...

Embodiment 2

[0081] Embodiment 2 actual sample detection

[0082] 1. Analytical instruments and reagents

[0083] Analytical instruments: SHIMADZU-8050 triple quadrupole mass spectrometer, equipped with ESI electrospray ion source, analysis software is Labsolutions 5.93 data processing system; Shimadzu LC20AD XR high performance liquid chromatography, equipped with Shimadzu Nexera X2 autosampler; The chromatographic column is a GL Science Bio C18 chromatographic column (100×2.1 mm, 1.7 μm).

[0084] The experimental reagents are shown in Table 6:

[0085] Table 6

[0086]

[0087] 2. Experimental methods and results

[0088] 2.1 Pretreatment of samples containing Berol 185

[0089] Take 2.0ml of the sample to be tested in a 15ml centrifuge tube, add 8.0ml of methanol, vortex for 30s, take 1.0ml of the mixed solution in a 1.5ml centrifuge tube, centrifuge at 14000g for 10min, take the supernatant into a 1.5ml sample bottle, Loading analysis.

[0090] 2.2 Chromatographic separation ...

Embodiment 3

[0114] Embodiment 3 actual sample detection

[0115] 1. Analytical instruments and reagents

[0116] Analytical instruments: SHIMADZU-8050 triple quadrupole mass spectrometer, equipped with ESI electrospray ion source, analysis software is Labsolutions 5.93 data processing system; Shimadzu LC20AD XR high performance liquid chromatography, equipped with Shimadzu Nexera X2 autosampler; The chromatographic column is a GL Science Bio C18 chromatographic column (100×2.1 mm, 1.7 μm).

[0117] The experimental reagents are shown in Table 11:

[0118] Table 11

[0119]

[0120] Note: NA means not applicable.

[0121] 2. Experimental methods and results

[0122] 2.1 Pretreatment of samples containing Berol 185

[0123] Take 2.0ml of the sample to be tested in a 15ml centrifuge tube, add 8.0ml of methanol, vortex for 30s, take 1.0ml of the mixed solution in a 1.5ml centrifuge tube, centrifuge at 14000g for 10min, take the supernatant into a 1.5ml sample bottle, Loading analysis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for determining the content of Berol 185 in a sample by using liquid chromatography-tandem mass spectrometry. The method comprises the following steps: A, preparing Beranol 185 standard solutions with different concentrations, and carrying out liquid chromatography-tandem mass spectrometry detection; screening out quantitative ions according to a liquid chromatography-tandem mass spectrometry detection result; B, respectively extracting peak areas of quantitative ion peaks of standard substance solutions with different concentrations, and drawing a peak area- concentration standard curve; C, pretreating the sample, and performing liquid chromatography-tandem mass spectrometry detection by adopting the method in the step A; and D, according to the detection result of the sample, comparing with the standard curve to obtain the concentration of Berol 185 in the sample. The method has the advantages of simple sample pretreatment, fast sample introduction, realization of the simple, fast and accurate analysis of the Berol 185 content, good sensitivity, high recovery rate, and high precision and reproducibility.

Description

technical field [0001] The invention relates to the field of biological product detection, in particular to a method for determining the content of Berol 185 in a sample by liquid chromatography tandem mass spectrometry. Background technique [0002] Berol 185, chemical name C10-16 fatty alcohol polyoxyethylene polyoxypropylene ether, molecular formula is R-O-(C 2 h 4 O)n-(C 3 h 6 O)m-H: R=C n h 2n+1 (n=10-16), is a hydrophilic nonionic surfactant, non-UV absorbing, non-fluorescent, clear liquid, pH range 5-7 in 1% water, soluble in water, isopropanol , methanol, ethanol. [0003] On the one hand, Berol 185 is effective in controlling the endotoxin content after adding the surfactant in the production process of pertussis vaccine; on the other hand, Berol 185 is toxic to aquatic organisms. Therefore, it is particularly important for the safe production of vaccines to establish a method that can quickly, accurately, sensitively and reliably detect the amount of Berol 1...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/8631G01N30/8675G01N2030/324Y02A50/30
Inventor 殷寻邓海清尹珊珊刘建凯
Owner BEIJING MINHAI BIOTECH