Human angiotensin converting enzyme 2-based affinity polypeptide for severe acute respiratory syndrome coronavirus 2

An angiotensin, respiratory system technology, applied in the field of biomaterials, can solve the problems of limited use range of peptide chain length, unable to cover key sites, etc., and achieve the effect of good application prospects

Active Publication Date: 2021-02-19
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. Unable to cover the key sites of hACE2 and SARS-CoV-2 S protein RBD;
[0007] 2. The length of existing peptide chains limits its scope of use

Method used

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  • Human angiotensin converting enzyme 2-based affinity polypeptide for severe acute respiratory syndrome coronavirus 2
  • Human angiotensin converting enzyme 2-based affinity polypeptide for severe acute respiratory syndrome coronavirus 2
  • Human angiotensin converting enzyme 2-based affinity polypeptide for severe acute respiratory syndrome coronavirus 2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 SARS-CoV-2 affinity polypeptide design based on hACE2

[0042] 1. Recognition and remodeling of key action sites of hACE2 and SARS-COV-2S_RBD complex

[0043] The co-crystal structure of hACE2 and SARS-COV-2S_RBD complex was used as a reference for analysis and the principle of peptide inhibition.

[0044] figure 1 Shown is the crystal structure showing the co-crystallization of hACE2 and SARS-COV-2S_RBD complex. The crystal structure of the co-crystal of hACE2 and SARS-COV-2S_RBD complex was determined by X-ray crystallography analysis. Both hACE2 and SARS-COV-2 interact through the protein surface to form a complex. If the specific information on the binding surface of hACE2 and SARS-COV-2 is clear, specific polypeptides can be designed to compete for binding to the surface of SARS-COV-2, thereby preventing the binding of hACE2 and achieving the purpose of inhibiting SARS-COV-2.

[0045] figure 1 (A) S_RBD is shown in green, hACE2 is shown in blue. ...

Embodiment 2

[0074] Example 2 SARS-COV-2S_RBD Rapid Biodetection Method Based on Affinity Peptide Design

[0075] According to the SARS-CoV-2S_RBD affinity polypeptide obtained in Example 1, the affinity polypeptide Lib15-2 and Lib12-1 with non-overlapping binding sites were selected as biorecognition materials, and the method for rapid biological detection of SARS-CoV-2S_RBD was designed .

[0076] The affinity polypeptide Lib15-2 and Lib12-1 were respectively treated with -CONH-(CH 2 ) 7 -Cys modification, the affinity peptides Lib15-2 and Lib12-1 were modified onto gold nanoparticles (AuNPs) by a co-bonding method to obtain two functional biosensing materials, AuNPs probes (peptide-AuNPs), and the above Two peptide-functionalized modified gold nanoparticles were mixed in a 1:1 ratio to form a biodetection reagent. The specific steps include: 1) using the polypeptide solid-phase synthesis method to synthesize the polypeptide, and inserting the linker 8-aminocaprylic acid and cysteine ...

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Abstract

The invention provides a human angiotensin converting enzyme 2-based affinity polypeptide for the severe acute respiratory syndrome coronavirus 2, and relates to the technical field of biological materials. Key human angiotensin converting enzyme 2 amino acid residues of binding sites of human angiotensin converting enzyme 2 and the severe acute respiratory syndrome coronavirus 2 are extracted toreconstruct a peptide library, and the human angiotensin converting enzyme 2-based affinity polypeptide for the severe acute respiratory syndrome coronavirus 2 is obtained by screening; and then, a biological detection reagent is prepared from the affinity polypeptide, and thus, a new method is provided for detection of the severe acute respiratory syndrome coronavirus 2.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a severe acute respiratory syndrome coronavirus 2 affinity polypeptide based on human angiotensin-converting enzyme 2. Background technique [0002] Existing studies have found that SRAS-CoV-2 virus belongs to coronavirus, and the spike (S) protein on its surface plays an important role in the process of virus infection of cells. S protein is an oligomeric transmembrane protein consisting of two subunits, S1 and S2. The receptor binding domain (S_RBD) in the S1 subunit binds to the human functional receptor ACE2 (hACE2-human angiotensin-converting enzyme 2), which in turn triggers the binding of the S2 subunit to the host cell membrane for fusion. Studies have shown that the binding affinity between SARS-CoV-2 S_RBD and hACE2 can reach the nanomolar level, and this high binding affinity may be one of the reasons why SRAS-CoV-2 is extremely easy to spread among the populatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/48C07K1/04G01N33/569G01N33/532
CPCC12N9/485C12Y304/17023G01N33/56983G01N33/532G01N2333/165
Inventor 周小红朱茜
Owner TSINGHUA UNIV
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