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Ion exchange resin for purification of deoxyribonucleoside triphosphate and preparation method thereof

A technology of deoxyribonucleoside triphosphate and ion exchange resin, applied in the field of ion exchange resin for purification of deoxyribonucleoside triphosphate and its preparation, ion exchange resin for purification of deoxyribonucleoside triphosphate, can solve the problem of low mechanical strength , Large pore size distribution range, small exchange capacity, etc., to achieve improved yield, simple and efficient method, and good purity

Active Publication Date: 2022-07-19
潍坊华诺医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art ion exchange resin for deoxyribonucleoside triphosphate purification has the following main defects: it is difficult to increase the specific surface area of ​​the resin, the exchange capacity is relatively small, the mechanical strength is low, the pore size distribution range is large, and the sphericity is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 A kind of ion exchange resin for purification of deoxyribonucleoside triphosphate and preparation method thereof

[0041] The preparation method of ion exchange resin of the present invention comprises the following steps:

[0042] Step 1. Preparation of porogen solution

[0043] The porogen was added into deionized water at 15wt% and stirred until completely dissolved, and 5wt% (deionized water) emulsifier span-60 was added and stirred rapidly for 40min to obtain a porogen solution.

[0044] The components of the porogen and their respective parts by weight are: 100 parts of chitosan, 15 parts of urea, 10 parts of chitosan oligosaccharide and 1 part of calcium chloride.

[0045] The chitosan has a viscosity of 15-20cps and a degree of deacetylation of 77-80%;

[0046] The calcium chloride, CAS No. 10043-52-4, has an active ingredient content of 99.0% and a phosphate content of 0.003%;

[0047] The chitosan oligosaccharide has a molecular weight of 1700-...

Embodiment 2

[0060] Embodiment 2 A kind of ion exchange resin for purification of deoxyribonucleoside triphosphate and preparation method thereof

[0061] The preparation method of ion exchange resin of the present invention comprises the following steps:

[0062] Step 1. Preparation of porogen solution

[0063] The porogen was added into deionized water at 15wt% and stirred until completely dissolved, and 5wt% (deionized water) emulsifier span-60 was added and stirred rapidly for 30min to obtain a porogen solution.

[0064] The components of the porogen and their respective parts by weight are: 110 parts of chitosan, 8 parts of urea, 14 parts of chitosan oligosaccharide and 0.5 part of calcium chloride.

[0065] The chitosan has a viscosity of 25-40cps and a degree of deacetylation of 75-80%;

[0066] The calcium chloride, CAS No. 10043-52-4, has an active ingredient content of 99.4% and a phosphate content of 0.001%;

[0067] The chitosan oligosaccharide has a molecular weight of 1700...

Embodiment 3

[0080] Embodiment 3 A kind of ion exchange resin and preparation method thereof

[0081] The preparation method of ion exchange resin of the present invention comprises the following steps:

[0082] Step 1. Preparation of porogen solution

[0083] The porogen was added to deionized water at 20wt% and stirred until completely dissolved, and 5wt% (deionized water) emulsifier span-60 was added and stirred rapidly for 25min to obtain a porogen solution.

[0084] The components of the porogen and their respective parts by weight are: 120 parts of chitosan, 8 parts of urea, 20 parts of chitosan oligosaccharide and 0.1 part of calcium chloride.

[0085] The chitosan has a viscosity of 42-45cps and a degree of deacetylation of 70-74%;

[0086] The calcium chloride, CAS No. 10043-52-4, has an active ingredient content of 99.2% and a phosphate content of 0.001%;

[0087] The chitosan oligosaccharide has a molecular weight of 1600-1700 Da and a degree of deacetylation of 95.7%.

[00...

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PUM

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Abstract

The present invention provides an ion exchange resin for deoxyribonucleoside triphosphate purification and a preparation method thereof, including preparation of porogen solution, base material preparation, base material impregnation and resin synthesis. 100-120 parts of polysaccharides, 8-15 parts of urea, 10-20 parts of chitosan oligosaccharides and 0.1-1 parts of calcium chloride; Water and methylene blue are stirred and mixed in a weight ratio of 10:0.1-1:30-50:12-18, kept at 50-60° C. for 1-2 hours, and then added to the oil phase reaction; the ion exchange resin of the present invention, Large specific surface area, large exchange capacity, high mechanical strength, uniform pore size distribution, high sphericity, specific surface area of ​​90‑130g / m 2 , the total exchange capacity is 16.2-21.4mmol / g, and the volume exchange capacity is 4.4-7.1mmol / ml.

Description

technical field [0001] The invention belongs to the technical field of resin preparation, and relates to an ion exchange resin for deoxyribonucleoside triphosphate purification, in particular to an ion exchange resin for deoxyribonucleoside triphosphate purification and a preparation method thereof. Background technique [0002] Deoxyribonucleotide is the basic unit of DNA structure. Deoxyribonucleotide or its salt is traditionally obtained from deoxyribonucleic acid (DNA) through the enzymatic hydrolysis of 5'-phosphodiesterase. It has a wide range of applications in medicine, reagents, fine chemicals and other fields. dNTP, short for deoxy-ribonucleoside triphosphate. It is a general term including dATP, dGTP, dTTP, dCTP, etc., N refers to nitrogenous base, and the representative variable refers to one of A, T, G, C, etc. In biological DNA synthesis, as well as various PCR (RT-PCR (reverse transcription PCR), Real-time PCR) as a raw material. The prior art ion exchange ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/08C08F212/36C08J9/40C08J9/26C08L25/08
CPCC08F212/08C08J9/40C08J9/26C08J2325/08C08J2201/0422C08J2201/0424C08J2201/0444C08F212/36
Inventor 张国臣刘维华刘建亮于敦海
Owner 潍坊华诺医药科技有限公司