Lysostaphin and chitosan composite antibacterial film spraying agent

Abstract

The invention discloses a lysostaphin and chitosan composite antibacterial film spraying agent. The composite gel is prepared from the following raw materials in percentage by massic volume: 1%-20% oflysostaphin, 0.1%-5% of chitosan, 0.5%-10% of a surface tension regulator, 0.5%-5% of a pH regulator and the balance of medical purified water. The preparation method comprises the following preparation steps: S1, adjusting the pH value of the medical purified water under a sterile condition; S2, adding chitosan into the mixed solution obtained in the S1, and carrying out mixing to obtain a solution; and S3, adding the lysostaphin into the solution obtained in the S2. The composite antibacterial film spraying agent can quickly form films, is long in maintaining time, can quickly stop bleeding, coagulate blood and form water-insoluble protective films on wound surfaces, has certain air permeability, is wide in antibacterial range and non-irritant to skin, is helpful for forming the water-insoluble protective films by adding the surface tension regulator, and is not prone to film forming.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a composite antibacterial film spraying agent of lysostaphin and chitosan. Background technique [0002] We often encounter small and medium wounds in daily life, and the wounds are bleeding, swelling, and accompanied by bacterial infections. At present, people often use band-aids, iodophors, or antibacterial and anti-inflammatory sprays for treatment, but there are often inconveniences in post-treatment care, poor wound healing ability, Wounds exposed to water will aggravate the disadvantages of infection. If the wound is not effectively protected and treated, it will cause secondary infection of wound bacteria, leading to wound ulceration, sepsis and other adverse consequences. Lambert-negative pathogens such as Pseudomonas aeruginosa are dangerous species resistant to multiple antibiotics. In recent years, with the extensive use of antibiotics, th...

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Problems solved by technology

However, a problem with common film spraying agents is that the film formed on the surface of the wound after the liquid is sprayed is affected by the surface tension and cannot fit well with the wound. However, various spray film agents on the market still have various problems.

[0004] Antimicrobial peptide spray film-forming agent disclosed in CN1899600B and preparation method thereof as authorized announcement number, although it has realized that antimicrobial peptide spray film-former is sprayed on the affected part and forms a layer of thin film within a few minutes, it not only plays a direct role in the affected part Protective effect, quickly kill bacteria and prevent further infection of the affected area, and the film has good affinity and air permeability to the skin; the spray film-forming agent contains humectant ingredients, which can absorb the exudate and water of the affected area, and play a more effective role. Good moisturizing effect can have a good therapeutic effect on burn infection, but it does not solve the problem that the existing spray film agent cannot form a breathable film when it is used, it is easy to dissolve in water, it is easy to cause irritation to the skin, and it is easy to produce a film problems such as shedding, for this reason we propose a lysostaphin and chitosan composite antibacterial spray film agent

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