Preparation method of phlebopus portentosus polysaccharide

A technology of boletus and polysaccharides, which is applied in the field of preparation of boletus bursa polysaccharides, can solve the problems of difficult structure and composition research, low polysaccharide purity, etc., to improve yield and purity, reduce volume, and facilitate centrifugal effect

Inactive Publication Date: 2021-03-09
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of the polysaccharide obtained by the current separation and purification methods of Boletus pheoides polysaccharides is low, and it i

Method used

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  • Preparation method of phlebopus portentosus polysaccharide
  • Preparation method of phlebopus portentosus polysaccharide
  • Preparation method of phlebopus portentosus polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Extraction and pretreatment of polysaccharides

[0043] Under sterility conditions, a punch having a diameter of 10 mm is punched on a pneumatic dish of the dark brownwheld. The pentarami is inoculated with a five-strand of five strains inoculated in 200 ml of liquid fermentation medium. In a triangular bottle, 25 ° C, 100R / min constant temperature culture 10d, after removal of the filament, combined with the fermentation liquid at 0.08 MPa, 60 ° C for 10 min. The quantitative decorate shank boletus fermentation liquid (6L, each time there are multiple 200 ml fermentation medium while fermenting, totaling 4 batches), concentrated at 0.08 MPa, 60 ° C for 10 min, concentrate, first centrifugal treatment, The supernatant was combined, and then put three times of volume ethanol on the magnetic stirrer, sealing the fresh film, and placed in a refrigerator to precipitate 24 h. After alcohol sink, the centrifugation of the centrifugation was 8000 rpm, 15 min, and after centrifug...

Embodiment 2

[0054] Extraction and pretreatment of polysaccharides

[0055] Under sterility conditions, a punch having a diameter of 10 mm is punched on a pneumatic dish of the dark brownwheld. The pentarami is inoculated with a five-strand of five strains inoculated in 200 ml of liquid fermentation medium. In a triangular bottle, 32 ° C, 200R / min constant temperature culture 15d, after removal, filtrate, mixed with fermentation, concentrated at 0.09 mPa, 40 ° C. The quantitative is taking a dark brown pair of boletus fermentation liquid (6L, each time there are multiple 200 ml fermentation medium, and 4 batches have been completed, concentrated in 0.09 MPa, 40 ° C for 20 min, and concentrate. Centrifugal treatment. The supernatant was combined, and then a 5x volume ethanol was placed on a magnetic stirrer, sealed with a plastic film, and placed in a refrigerator precipitate 24 h. After alcohol sink, the centrifugation was carried out by 8000 rpm, 15 min; after centrifugal ethanol precipitat...

Embodiment 3

[0066] Extraction and pretreatment of polysaccharides

[0067] Under sterility conditions, a punch having a diameter of 10 mm is punched on a pneumatic dish of the dark brownwheld. The pentarami is inoculated with a five-strand of five strains inoculated in 200 ml of liquid fermentation medium. In a triangular bottle, 28 ° C, 150 r / min constant temperature culture was removed, and then filtered off the bacteria, and then the fermentation liquid was mixed at 0.085 MPa, 50 ° C for 15 min. The quantity was taking a fermentation of boletus (6L, each time with a plurality of 200 ml of fermentation medium while fermenting, a total of 4 batches), concentrated in 0.085 MPa, 50 ° C, concentrated to centrifugal treatment, The supernatant was combined, and 4x volume ethanol was placed on a magnetic stirrer, sealed with a fresh film, and placed in a refrigerator precipitate 24 h. After centrifugation, centrifugation was carried out after alcohol, and the centrifugation was 8000 rpm, centrif...

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Abstract

The invention discloses a preparation method of phlebopus portentosus polysaccharide. The preparation method comprises the following steps: inoculating phlebopus portentosus hyphae into a liquid fermentation culture medium, and fermenting to obtain fermentation broth; concentrating the obtained fermentation broth to obtain a concentrated solution; centrifuging the obtained concentrated solution, taking the supernatant, adding ethanol into the supernate, centrifuging, collecting the precipitate, and carrying out freeze drying to obtain crude polysaccharide; preparing a crude polysaccharide solution, eluting the crude polysaccharide solution by using a DEAE-52 cellulose column, and concentrating the eluent to obtain a crude polysaccharide concentrated solution; and loading the obtained crudepolysaccharide concentrated solution into a dialysis bag for dialysis, and freeze-drying the dialysate to obtain the phlebopus portentosus polysaccharide. According to the method, the purity of the phlebopus portentosus polysaccharide is improved, the subsequent research on the structure and composition of the fungal polysaccharide is facilitated, and the development space and way are broadened for the development of the medicinal value of the phlebopus portentosus polysaccharide.

Description

Technical field [0001] The present invention relates to the field of polysaccharide extraction, and more particularly Background technique [0002] Dark Brown Person Burgin [Phralebopus Portentosus (Berk. & Broome) Boedijn] is a Boiletinellaceae, a polarized boletus fungi, but also known as dark brown net boletus, commonly known as " Black boletus. Mainly distributed in China, Hainan, Yunnan and Guangxi. The dark brown pair of boletus is not only good, but also contains various types of nutrients, such as amino acids, polysaccharides, proteins, etc., is a high nutrient ingredient. [0003] The dark brown pastelet contains a large number of fungal polysaccharides, experimental studies have found that fungal polysaccharides have a protective effect on the heart of mice, and can significantly inhibit kidney cancer cells in mice. However, the separation of buseptic boletus polysaccharides, the purification method is low, and it is difficult to study the structure and composition of t...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 曾念开王勇吴莉陈晓娜谢惠菁张玉卓徐畅张絮
Owner HAINAN MEDICAL COLLEGE
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