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Anoectochilus formosanus polyploid induction method

A polyploid, clematis technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems that have not yet been reported, and achieve reduced physical injury, high induction efficiency, and improvement of low survival rate phenomenon. Effect

Active Publication Date: 2021-03-16
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0005] At present, the technology of using Oryzalin to induce polyploidy has been successfully applied to the breeding of various medicinal plants, and many new germplasm resources have been obtained. However, the research on Oryzalin-induced polyploidy of Aurantia chinensis has not been reported at home and abroad

Method used

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  • Anoectochilus formosanus polyploid induction method
  • Anoectochilus formosanus polyploid induction method
  • Anoectochilus formosanus polyploid induction method

Examples

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Embodiment 1

[0031] (1) Inducer preparation: Dissolve 20 mg of amisulfuryl powder in 1 mL of dimethyl sulfoxide (DMSO) to prepare a mother solution with a concentration of 2%, and dilute the 0.002% amisulfuryl solution with purified water , sealed and stored in a refrigerator at 4°C after freeze-thaw sterilization.

[0032] (2) Pre-cultivation treatment of stem segments: select sterile test-tube seedlings with consistent growth and robust growth, cut 3-4 cm stem segments with multiple bud points in an ultra-clean workbench, and insert MS+1 mg / In the medium of L 6-BA+0.1 mg / L NAA+0.5 mg / L KT+0.5g / L AC+30g / L sucrose, pre-cultivate for 3 days until the lateral buds of the stem segment appear white bud spots. The survival rate reached 72%.

[0033] (3) Inducing agent soaking treatment: Soak the long stems that have sprouted in 0.002% amisulamide solution, place them under 23 degrees Celsius and 12 hours of light, soak for 108 hours, and shake them every 6 hours to make the material and Full...

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Abstract

The invention provides an anoectochilus formosanus polyploid induction method. The anoectochilus formosanus polyploid induction method comprises the steps of inducer preparation, stem pre-culture treatment, inducer soaking treatment, screening culture and the like. According to the method, the chromosome doubling induction efficiency is higher, the phenomenon of low explant survival rate caused bycolchicine induction is effectively improved, and due to the use of a low-concentration inducer, plants can grow healthily and efficiently, and the harm to the body of an operator can also be reduced.

Description

technical field [0001] The invention relates to the fields of horticultural plant propagation and new variety cultivation, in particular to a method for inducing polyploidy of Aurea clematis. Background technique [0002] Most of the new varieties of clematis are obtained directly from outdoor wild strains, and the explants are sterilized to multiply the tissue culture seedlings. There are fewer ways to obtain new varieties through crossbreeding. In the polyploid breeding of clematis, colchicine is often used to induce the doubling of plant chromosomes, thereby obtaining polyploid plants. For example, if 0.1% colchicine solution soaks the stems of A. roliscens for 48 hours, the mutagenesis rate can reach 48%. Under living conditions, a certain concentration of amisulfurin (Oryzalin) is used to induce doubling, mainly by dipping, dripping, smearing, covering, injection or capillary methods. Oryzalin is used to induce doubling in vitro, mainly by soaking or embedding. It h...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/008
Inventor 石研曾黎辉曾敏婕
Owner FUJIAN AGRI & FORESTRY UNIV
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