Anoectochilus formosanus polyploid induction method

A polyploid, clematis technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems that have not yet been reported, and achieve reduced physical injury, high induction efficiency, and improvement of low survival rate phenomenon. Effect

Active Publication Date: 2021-03-16
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0005] At present, the technology of using Oryzalin to induce polyploidy has been successfully applied to the breeding of various medicinal plants, and many n

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  • Anoectochilus formosanus polyploid induction method
  • Anoectochilus formosanus polyploid induction method
  • Anoectochilus formosanus polyploid induction method

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[0030] Example 1

[0031] (1) Inducing agent preparation: First, 20 mg ammonia disineth is first dissolved in 1 ml dimethyl sulfoxide (DMSO), formulated a miperm for concentration of 2%, and diluted the concentration of 0.002% by pure water. After freezing, it is stored in 4 degrees Celsius refrigerators.

[0032] (2) Pre-culturing treatment of stem segment: Select a growing, growing aseptic test tube seedlings, cut into three-4 cm in the ultra-net table with a plurality of sprouts, access MS + 1 mg / L 6-BA + 0.1 mg / l NaA + 0.5 mg / L KT + 0.5 g / l AC + 30g / L of sucrose medium, pre-cultured 3 days, the naked eye can be seen in the side bud portion of the stem segment. The survival rate reached 72%.

[0033] (3) Soak treatment of inducers: soak the long budged long stem segment in 0.002% ammonia, placed under 23 degrees Celsius, 12 hours of light, soaking treatment 108h, shake once every 6 hours, make the material The liquid is fully contacted. After the soaking, the stem seg...

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Abstract

The invention provides an anoectochilus formosanus polyploid induction method. The anoectochilus formosanus polyploid induction method comprises the steps of inducer preparation, stem pre-culture treatment, inducer soaking treatment, screening culture and the like. According to the method, the chromosome doubling induction efficiency is higher, the phenomenon of low explant survival rate caused bycolchicine induction is effectively improved, and due to the use of a low-concentration inducer, plants can grow healthily and efficiently, and the harm to the body of an operator can also be reduced.

Description

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Claims

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Application Information

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Owner FUJIAN AGRI & FORESTRY UNIV
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