Anoectochilus formosanus polyploid induction method
A polyploid, clematis technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems that have not yet been reported, and achieve reduced physical injury, high induction efficiency, and improvement of low survival rate phenomenon. Effect
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[0030] Example 1
[0031] (1) Inducing agent preparation: First, 20 mg ammonia disineth is first dissolved in 1 ml dimethyl sulfoxide (DMSO), formulated a miperm for concentration of 2%, and diluted the concentration of 0.002% by pure water. After freezing, it is stored in 4 degrees Celsius refrigerators.
[0032] (2) Pre-culturing treatment of stem segment: Select a growing, growing aseptic test tube seedlings, cut into three-4 cm in the ultra-net table with a plurality of sprouts, access MS + 1 mg / L 6-BA + 0.1 mg / l NaA + 0.5 mg / L KT + 0.5 g / l AC + 30g / L of sucrose medium, pre-cultured 3 days, the naked eye can be seen in the side bud portion of the stem segment. The survival rate reached 72%.
[0033] (3) Soak treatment of inducers: soak the long budged long stem segment in 0.002% ammonia, placed under 23 degrees Celsius, 12 hours of light, soaking treatment 108h, shake once every 6 hours, make the material The liquid is fully contacted. After the soaking, the stem seg...
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