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Composition containing resorcinol or phenethyl resorcinol for preventing, treating or ameliorating skin diseases induced by air pollutants

A technology of phenylethylresorcinol and air pollutants, which is applied in the field of skin disease compositions, can solve problems such as unclearness, and achieve the effect of inhibiting skin irritation

Pending Publication Date: 2021-03-19
RES & BUSINESS FOUND SUNGKYUNKWAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inhibitory effect on skin irritation induced by air pollutants is unclear

Method used

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  • Composition containing resorcinol or phenethyl resorcinol for preventing, treating or ameliorating skin diseases induced by air pollutants
  • Composition containing resorcinol or phenethyl resorcinol for preventing, treating or ameliorating skin diseases induced by air pollutants
  • Composition containing resorcinol or phenethyl resorcinol for preventing, treating or ameliorating skin diseases induced by air pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Embodiment 1: confirm that resorcinol (Resorcinol) or phenylethylresorcinol (Phenylethyl resorcinol) cytotoxicity

[0129] The human immortalized keratinocyte (Human immortalized keratinocyte) (HaCaT) cells were divided into 1×10 per well (well). 5 Each standard was inoculated onto a 6-well plate (6-well plate) using DMEM medium containing 10% FBS (fetal bovine serum), and then incubated in 5% CO 2 and 37° C. until the cells adhere to more than 80% of the bottom surface of the well plate.

[0130] After culturing, HaCaT cells were treated with benzo(a)pyrene (Benzo(α)pyrene) [purchase: Sigma-Aldrich, ≥96% (HPLC)] according to 15 μM standard. After 1 hour, resorcinol (Resorcinol) [purchased: Sigma-Aldrich, ACS reagent] was used for post-treatment at different concentrations (0.5 mM, 1 mM, 3 mM). Then, in 5% CO 2 , and cultured at 37°C for about 1 day. Next, cytotoxicity was measured by the CCK-8 (Cell Counting Kit-8) assay method. At the same time, for compariso...

Embodiment 2

[0135] Example 2: Confirmation of gene expression levels related to skin irritation caused by resorcinol treatment

[0136] 2-1: Confirm Xenobiotics Response Element (XRE)-luciferase reporter activity inhibition

[0137] Human immortalized keratinocyte (HaCaT) cells were placed on each well at 1×10 5 Each standard was inoculated onto a 6-well plate (which uses DMEM medium containing 10% FBS), and then in 5% CO 2 and cultured at 37°C until the cells adhered to about 50% at the bottom of the well plate.

[0138] After culturing, remove 20% of the medium, and use 1 μg of β-galactosidase vector (β-galactosidase vector) and XRE promoter (XRE promoter) to clone HaCaT cells on luciferase vector (luciferase vector). 1 μg of XRE reporter plasmid (XRE-reporter plasmid), then utilize 5 μg PEI (polyethyleneimine; Polyethy lenimine) to make 1 μg of XRE reporter gene plasmid transduction, then in 5% CO 2 and incubated at 37°C for 4 hours. Then, replace with new medium, followed by 5% ...

Embodiment 3

[0153] Example 3: Confirmation of the expression level of skin irritation-related genes caused by phenylethyl resorcinol treatment

[0154] 3-1: Confirm Xenobiotics Response Element (XRE)-luciferase reporter activity inhibition

[0155] According to the same experimental method as in Example 2-1, replace resorcinol with phenylethylresorcinol (Phenylethylresorcinol) [purchase: Sigma-Aldrich, ACS reagent] at different concentrations (1uM, 50uM, 100uM ) after treatment, the XRE reporter gene (XRE reporter) activity was measured.

[0156] The result is as Figure 2a As shown, it can be seen from the figure that the XRE reporter activity induced by benzo(a)pyrene is inhibited as the treatment concentration of phenylethyl resorcinol increases. Among them, the inhibitory effect on XRE reporter activity is the most obvious under the condition of 100uM concentration of phenylethyl resorcinol.

[0157] From this, it can be seen that phenethylresorcin suppresses the effect of benzo(...

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Abstract

The invention relates to a composition containing resorcinol or phenethyl resorcinol for preventing, treating or ameliorating skin diseases induced by air pollutants. The resorcinol or phenethyl resorcinol according to the present invention has the effects of inhibiting expression of XRE induced by air pollutants such as benzo (a) pyrene and fine dust, expression of protein or mRNA of CYP1A1, or movement of AhR into a nucleus, and the like, thereby confirming that the resorcinol or phenethyl resorcinol can inhibit skin irritation. Therefore, the resorcinol or phenethyl resorcinol of the present invention can be effectively used in the field of research and development of therapeutic agents or ameliorating agents for various skin diseases induced by air pollutants.

Description

[0001] Statement on Government Powers [0002] The cutting-edge biomaterial commercialization support plan (code#: B0080507000574) supported by the Ministry of Industry, Trade and Energy and Korea Industrial Technology Research Institute is the subject of "support for the commercialization and mass production of biosynthetic functional materials derived from natural products". technical field [0003] The invention relates to a composition containing resorcinol or phenylethylresorcinol for preventing, treating or improving skin diseases induced by air pollutants. Background technique [0004] The skin is the largest organ of the human body composed of three layers: subcutaneous tissue, dermis, and epidermis. It has the function of protecting the human body from physical, chemical, and biological factors. External pollutants such as heavy metal powder and fine dust caused by rapid industrialization, automobile exhaust, and air masses moving from China can contaminate human sk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P17/00A61P37/08A61P17/02A61P17/06A61P31/04A61P31/10A61P17/08A61P25/00A61K8/34A61Q19/00A61Q19/10A61Q1/14A61Q5/12A61Q1/02
CPCA61K31/05A61P17/00A61P37/08A61P17/02A61P17/06A61P31/04A61P31/10A61P17/08A61P25/00A61K8/347A61Q19/00A61Q19/10A61Q1/14A61Q5/12A61Q1/02A61Q17/00
Inventor 李钟星李承恩
Owner RES & BUSINESS FOUND SUNGKYUNKWAN UNIV
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