Cell incubator capable of controlling carbon dioxide concentration

Abstract

The invention discloses a cell incubator capable of controlling carbon dioxide concentration. The cell incubator comprises a constant temperature incubator, a plurality of transverse plates and a plurality of culture dishes arranged on the transverse plates, wherein the transverse plates are slidably arranged on the inner side wall of the constant temperature incubator; the inner side wall of theconstant temperature incubator is fixedly connected with a plurality of absorption plates; and the outer side wall of the constant temperature incubator is fixedly connected with a treatment box. According to the invention, under the mutual cooperation of the absorption plates, the treatment box, a communication groove, a carbon dioxide absorption bottle, a guide device, an air injection device and an absorption device, air in the constant temperature incubator is injected into a sodium hydroxide solution in the carbon dioxide absorption bottle through the air injection device, so that the sodium hydroxide solution absorbs carbon dioxide, the concentration of the carbon dioxide in the constant temperature incubator is reduced, and the pH value of a culture solution is increased; and compared with a traditional process that a sodium bicarbonate solution and the carbon dioxide are added to control the pH value of the culture solution, the cell incubator saves more time and labor and is more convenient.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture box capable of controlling carbon dioxide concentration. Background technique [0002] The carbon dioxide incubator is to form a growth environment similar to cells / tissues in the organism by simulating in the incubator box. The incubator requires a stable temperature (37°C), a stable carbon dioxide level (5%), and a constant pH value (pH value : 7.2-7.4), higher relative saturation humidity (95%), a device for culturing cells / tissues in vitro. [0003] Because the medium gas environment of the cell incubator is 95% air and 5% carbon dioxide. Carbon dioxide is not only a product of cell metabolism, but also a component required by cells. It is mainly related to maintaining the pH of the culture medium. Most animal cells require a slightly alkaline environment. The pH is 7.2 to 7.4, preferably not exceeding 6.8 to 7.6. During the cultivation process, as the r...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that traditional cell culture in the prior art adds sodium bicarbonate solution and carbon dioxide to the culture fluid to maintain the pH value of the culture fluid.

Images