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Methods to detect 7 carotenoids

A technology of carotenoids and species, applied in the field of analytical chemistry, can solve problems such as the inability to quickly and accurately detect carotenoids

Active Publication Date: 2022-07-05
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this application is to provide a method for detecting 7 carotenoids, aiming at solving the problem of being unable to quickly and accurately detect 7 or more carotenoids in the prior art

Method used

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  • Methods to detect 7 carotenoids
  • Methods to detect 7 carotenoids
  • Methods to detect 7 carotenoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Standard curve for preparing standards

[0091] Preparation of mixed standards of fucoxanthin standard, fucoxanthin standard, diaoxanthin standard, diaoxanthin standard, neoxanthin standard, zeaxanthin standard, violaxanthin standard Solution: Dissolve 7 carotenoid standards (all 1.0 mg) in 1 mL of methanol to obtain 7 standard mother solutions with a single concentration of 1 mg / mL; accurately measure the individual standard mother solutions and dilute with methanol to obtain 7 kinds of standard working solutions with a concentration of 10 μg / mL; respectively accurately measure a single standard working solution, and mix to obtain a mixed standard solution. , The concentration of diatomoxanthin is 500ng / mL, and the concentration of zeaxanthin, neoxanthin and violaxanthin is 100ng / mL.

[0092] Further, diluted with methanol in turn, wherein, fucoxanthin, fucoxanthin, diacoxanthin, and diacoxanthin at 500ng / mL, 250ng / mL, 125ng / mL, 50ng / mL, 25ng / mL, 12.5ng / mL, 5ng / mL, 2...

Embodiment 2

[0096] Detection of 7 carotenoids in fresh algae

[0097] Prepare the sample to be tested: provide 0.2 mg of algae raw material, add the algae raw material to 2 ceramic beads, and perform grinding at 25 Hz for 2 minutes to obtain algal raw material powder; mix the algal raw material powder with 2 mL of methanol solution and centrifuge, The algal raw material supernatant is obtained; the algal raw material supernatant is filtered through a 0.22 μm filter membrane to obtain a sample to be tested.

[0098] The samples to be tested were detected by ultra-high performance liquid chromatography-mass spectrometry. The test conditions of ultra-high performance liquid chromatography-mass spectrometry were as follows: Ultra-high performance liquid chromatography was detected by ultra-high performance liquid chromatograph ACQUITY Ultra Performance LC H-Class. The conditions of liquid chromatography detection are: the chromatographic column is HSS T3, wherein, the size of HSS T3 is 2.1mm*...

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Abstract

The present application relates to the technical field of analytical chemistry, and provides a method for detecting 7 carotenoids, simultaneously detecting fucoxanthin, fucoxanthin, diacoxanthin, diacoxanthin, neoxanthin, and zeaxanthin. 7 different carotenoids such as violaxanthin are detected. First, the standard curve of 7 carotenoids is provided, and then a small amount of samples are provided for detection. According to the retention time and quantitative ion pair of the mass spectrum of the sample to be tested The types of carotenoids can be determined according to the value of the qualitative ion pair, and the content of various carotenoids can be calculated according to the mass spectrum peak area of ​​the quantitative ion pair and the standard curve. The detection method requires a small amount of samples, has rapid detection, high efficiency, high sensitivity and good reproducibility, reduces experimental errors and improves the accuracy of data, and can realize fast and accurate large-throughput detection.

Description

technical field [0001] The application belongs to the technical field of analytical chemistry, and in particular relates to a method for detecting seven carotenoids. Background technique [0002] Carotenoids are a general term for a class of important natural pigments, which are commonly found in the yellow, orange-red or red pigments of animals, higher plants, fungi, and algae. Carotenoids are the main source of vitamin A in the body, and also have anti-oxidation, immune regulation, anti-cancer, anti-aging and other effects. [0003] At present, more than 700 kinds of natural carotenoids have been discovered, which can be divided into two categories according to their chemical structures. 2 Carotene and lycopene); the other is lutein (with hydroxyl, ketone, carboxyl, methoxy and other oxygen-containing functional groups, such as lutein and astaxanthin, etc.). Due to the variety of carotenoids and their similar structures, it is difficult to completely separate them during...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N30/8634
Inventor 陈峰刘宾
Owner SHENZHEN UNIV
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