Application of pyracantha fortuneana polysaccharide, pharmaceutical composition containing pyracantha fortuneana polysaccharide and drug-loaded vesicle containing pharmaceutical composition
A technology of Pyracantha polysaccharide and its composition, which is applied in the field of anti-tumor drug preparations, combined medication of Pyracantha polysaccharide and chemotherapeutic drugs, and can solve the problems of slow effect, drug effect that needs to be improved, and mild action.
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[0044] In addition, the present invention provides a method for preparing the above-mentioned drug-loaded vesicles, comprising the following steps: inducing apoptosis of tumor cells in the logarithmic growth phase, collecting the cell vesicles released by the apoptotic tumor cells, and feeding the cell vesicles Add an effective amount of the pharmaceutical composition as described above, and incubate for 16-24 hours to allow the pharmaceutical composition to enter the cell vesicles, and separate and purify the drug-loaded vesicles. The pharmaceutical composition is added in an amount close to the maximum saturation of the cell vesicles used as the carrier, and then incubated at room temperature to realize the encapsulation of the pharmaceutical composition by the cell vesicles, and the prepared drug-loaded vesicles have a uniform particle size, and the drug The composition has high loading capacity, good dispersibility and obvious tumor killing effect.
[0045] The method for ...
Embodiment 1
[0053] The synergistic effect of embodiment 1 pyracantha polysaccharide to doxorubicin
[0054] Tumor cell culture: the purified tumor cells were inoculated in RPMI 1640 medium containing 10% fetal bovine serum (v / v), 100 U / mL penicillin, 100 mg / mL streptomycin, at a temperature of 37 ° C, gas The atmosphere is 5% CO 2 Cultured under certain conditions, regularly observed and subcultured, digested when the tumor cells grew to the logarithmic phase, and then seeded the dispersed tumor cells in a 96-well plate and incubated overnight for later use.
[0055] Synergism experiment: Divide the tumor cells in the 96-well plate into 6 groups, 5 of which were used as the experimental group, and added 0.5 μg / mL doxorubicin, 400 μg / mL pyracantha polysaccharide, 0.5 μg / mL doxorubicin and 400 μg / mL pyracantha polysaccharide, 0.5 μg / mL doxorubicin and 400 μg / mL aloe polysaccharide, 0.5 μg / mL doxorubicin and 400 μg / mL astragalus polysaccharide, another group without any drug was used as th...
Embodiment 2
[0061] Example 2 Screening the appropriate concentration of Pyracantha polysaccharide combined with Adriamycin
[0062] The method of Example 1 was used for tumor cell culture, and then the tumor cells in the 96-well plate were divided into different groups, and 0.5 μg / mL doxorubicin and different concentrations of pyracantha polysaccharide (0, 50, 100, 200, 300 , 400μg / mL), and then the tumor cells were placed at a temperature of 37°C and a gas atmosphere of 5% CO 2 The tumor cells were cultured for 24 hours under certain conditions, and the MTT method was used to detect the inhibition of tumor cells in each group and calculate the inhibition rate. The test results are shown in Table 2.
[0063] Table 2 The inhibitory rate (%) of different concentrations of pyracantha polysaccharide and doxorubicin in combination to tumor cells
[0064]
[0065] Note: Compared with the 0.5μg / mL doxorubicin group, * P** P<0.01; " / " means not added.
[0066] It can be seen from Table 2 that...
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