Preparation method of bioactive surface coating for bone implantation polyester material as well as product and application
A surface coating and bioactive technology, which is applied in coating, tissue regeneration, drug delivery, etc., can solve the problems of poor compatibility and reduce the mechanical properties of bone repair scaffolds, so as to improve compatibility, have long-term effectiveness and Stability, the effect of improving biological activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] A bioactive surface coating for bone implant polyester materials, introducing amino groups on the polyester surface by surface hydrolysis to obtain an aminated polyester surface; using EDC / NHS coupling reaction to immobilize and load nano-hydroxyapatite The silk fibroin coating is prepared according to the following steps:
[0023] (1) Soak the degradable polyester stent in 50% (v / v) ethanol aqueous solution for 2 hours, then rinse with a large amount of water, and place the cleaned stent in 1,6-hexanediamine and isopropanol reacted at 45°C for 5 min, washed with deionized water for 12 h, and dried in vacuum for 24 h to obtain a scaffold with surface amination;
[0024] (2) Prepare an aqueous solution of EDC / NHS / MES cross-linking agent at a concentration of 20mM / 10mM / 10mM, and then prepare a mixed solution of 0.1g / mL silk fibroin and 0.05g / mL hydroxyapatite in SF / nHA; cross-linking agent After the aqueous solution and the SF / nHA mixed solution were mixed at a volume ra...
Embodiment 2
[0027] A bioactive surface coating for bone implant polyester material, which is similar to the steps in Example 1, and is prepared according to the following steps:
[0028] (1) Soak the degradable polyester stent in 50% (v / v) ethanol aqueous solution for 2 hours, then rinse with a large amount of water, and place the cleaned stent in 1,6-hexanediamine and isopropanol reacted at 45°C for 5 min, washed with deionized water for 12 h, and dried in vacuum for 24 h to obtain a scaffold with surface amination;
[0029] (2) Prepare an aqueous solution of EDC / NHS / MES cross-linking agent at a concentration of 20mM / 10mM / 10mM, and then prepare a mixed solution of 0.1g / mL silk fibroin and 0.1g / mL hydroxyapatite in SF / nHA; cross-linking solution After mixing with the SF / nHA mixed solution at a volume ratio of 1:9, immediately soak the aminated polyester scaffold in it and react at 37°C for 4h;
[0030] (3) After the reaction, the surface of the stent is washed with water and freeze-dried...
Embodiment 3
[0032] A bioactive surface coating for bone implant polyester material, which is similar to the steps in Example 1, and is prepared according to the following steps:
[0033] (1) Soak the degradable polyester stent in 50% (v / v) ethanol aqueous solution for 2 hours, then rinse with a large amount of water, and place the cleaned stent in 1,6-hexanediamine and isopropanol reacted at 45°C for 5 min, washed with deionized water for 12 h, and dried in vacuum for 24 h to obtain a scaffold with surface amination;
[0034] (2) Prepare an aqueous solution of EDC / NHS / MES cross-linking agent at a concentration of 20mM / 10mM / 10mM, and then prepare a mixed solution of 0.2g / mL silk fibroin and 0.2g / mL hydroxyapatite in SF / nHA; cross-linking solution After mixing with the SF / nHA mixed solution at a volume ratio of 1:9, immediately soak the aminated polyester scaffold in it and react at 37°C for 4h;
[0035] (3) After the reaction, the surface of the stent is washed with water and freeze-dried...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More