Preparation method and application of streptomyces and its biocontrol agent
A biocontrol agent and streptomyces technology, applied in the field of microorganisms, can solve the problems affecting the quality of walnut products, the height of walnut tree crowns, and soil environmental pollution, etc., to achieve slow development of drug resistance, strong ability to adapt to acid and alkali, and not destroy ecological balance Effect
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example 1
[0040] Step 1: Activate the strain. Streptomyces sandfield YNF36 preserved on the SYP medium slant was streaked on the SYP medium plate, and cultured upside down at 28°C for 3 days.
[0041] The formula of the SYP medium is: starch 10g / L, yeast extract powder 4g / L, peptone 10g / L, balance water, and the pH is adjusted to 7.2.
[0042] Step 2: Cultivation of the seed liquid: use a sterile puncher to punch out the fungus cake on the plate of the above-mentioned strain YNF36, inoculate it in the seed liquid medium, and cultivate it at 28° C. for 100 h at 120 r / min to obtain the seed liquid.
[0043] The formula of the seed liquid culture medium is: starch 10g / L, yeast extract powder 5g / L, peptone 5g / L, K 2 HPO 4 5g / L, NaCl 1g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7H 2 0.05g / L, surplus water, adjust pH to be 7.2.
[0044] Step 3: Solid fermentation culture of biocontrol agent: inoculate the seed liquid into solid fermentation medium according to the inoculum volume mass ratio of ...
example 2
[0048] Step 1: Activate the strain. Streptomyces sandfield YNF36 preserved on the SYP medium slant was streaked on the SYP medium plate, and cultured upside down at 28°C for 4 days.
[0049] The formula of the SYP medium is: starch 10g / L, yeast extract powder 4g / L, peptone 10g / L, balance water, and the pH is adjusted to 7.3.
[0050] Step 2: Cultivation of the seed liquid: use a sterile puncher to punch out the fungus cake on the plate of the above-mentioned strain YNF36, inoculate it in the seed liquid medium, and cultivate it at 28° C. and 180 r / min for 80 hours to obtain the seed liquid.
[0051] The formula of the seed liquid culture medium is: starch 10g / L, yeast extract powder 5g / L, peptone 5g / L, K 2 HPO 4 5g / L, NaCl 1g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7H 2 0.05g / L, surplus water, adjust pH to be 7.3.
[0052] Step 3: Solid fermentation culture of the biocontrol agent: inoculate the seed liquid into the solid fermentation medium according to the inoculum volume ma...
example 3
[0056] Step 1: Activate the strain. Streptomyces sandfield YNF36 preserved on the SYP medium slant was streaked on the SYP medium plate, and cultured upside down at 28°C for 5 days.
[0057] The formula of the SYP medium is: starch 10g / L, yeast extract powder 4g / L, peptone 10g / L, balance water, and the pH is adjusted to 7.4.
[0058] Step 2: Cultivation of the seed solution: use a sterile puncher to punch out the fungus cake on the plate of the above-mentioned strain YNF36, inoculate it in the seed solution medium, and cultivate it at 28° C. and 220 r / min for 50 hours to obtain the seed solution.
[0059] The formula of the seed liquid culture medium is: starch 10g / L, yeast extract powder 5g / L, peptone 5g / L, K 2 HPO 4 5g / L, NaCl 1g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7H 2 0.05g / L, surplus water, adjust pH to be 7.4.
[0060] Step 3: Solid fermentation culture of the biocontrol agent: inoculate the seed solution into the solid fermentation medium according to the inoculum vol...
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